Reclassification of Candida guilliermondii FTI 20037 as Candida tropicalis based on molecular phylogenetic analysis

Lima, Luanne Helena Augusto; Felipe, Maria das Graças de Almeida; Torres, Fernando Araripe Gonçalves

doi:10.1590/s1517-83822003000500033

Cited by 8 publications

(1 citation statement)

References 4 publications

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“…The yeast Candida tropicalis CCT 1516 was used in the experiments, and was donated by the Bioengineering Laboratory/UFPB from the Fundation Andre Tosello [ 47 ]. The pre-inoculum was made based on a mixture of 0.02 kg of rice bran in 1 L of water, which was autoclaved and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Simultaneous Production of Xylitol and Ethanol from Sisal Fiber

et al. 2018

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Recent years have seen an increase in the use of lignocellulosic materials in the development of bioproducts. Because sisal fiber is a low cost raw material and is readily available, this work aimed to evaluate its hemicellulose fraction for the simultaneous production of xylitol and ethanol. The sisal fiber presented a higher hemicellulose content than other frequently-employed biomasses, such as sugarcane bagasse. A pretreatment with dilute acid and low temperatures was conducted in order to obtain the hemicellulose fraction. The highest xylose contents (0.132 g·g−1 of sisal fiber) were obtained at 120 °C with 2.5% (v/v) of sulfuric acid. The yeast Candida tropicalis CCT 1516 was used in the fermentation. In the sisal fiber hemicellulose hydrolysate, the maximum production of xylitol (0.32 g·g−1) and of ethanol (0.27 g·g−1) was achieved in 60 h. Thus, sisal fiber presents as a potential biomass for the production of ethanol and xylitol, creating value with the use of hemicellulosic liquor without detoxification and without the additional steps of alkaline pretreatment.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the Simultaneous Production of Xylitol and Ethanol from Sisal Fiber

et al. 2018

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Xylitol dehydrogenase from Candida tropicalis: molecular cloning of the gene and structural analysis of the protein

Lima

Pinheiro

Moraes

et al. 2006

Appl Microbiol Biotechnol

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Yeasts can metabolize xylose by the action of two key enzymes: xylose reductase and xylitol dehydrogenase. In this work, we present data concerning the cloning of the XYL2 gene encoding xylitol dehydrogenase from the yeast Candida tropicalis. The gene is present as a single copy in the genome and is controlled at the transcriptional level by the presence of the inducer xylose. XYL2 was functionally tested by heterologous expression in Saccharomyces cerevisiae to develop a yeast strain capable of producing ethanol from xylose. Structural analysis of C. tropicalis xylitol dehydrogenase, Xyl2, suggests that it is a member of the medium-chain dehydrogenase (MDR) family. This is supported by the presence of the amino acid signature [GHE]xx[G]xxxxx[G]xx[V] in its primary sequence and a typical alcohol dehydrogenase Rossmann fold pattern composed by NAD(+) and zinc ion binding domains.

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Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition

Queiroz

Oliva

Silva

et al. 2022

Appl Microbiol Biotechnol

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Reclassification of Candida guilliermondii FTI 20037 as Candida tropicalis based on molecular phylogenetic analysis

Cited by 8 publications

References 4 publications

Evaluation of the Simultaneous Production of Xylitol and Ethanol from Sisal Fiber

Evaluation of the Simultaneous Production of Xylitol and Ethanol from Sisal Fiber

Xylitol dehydrogenase from Candida tropicalis: molecular cloning of the gene and structural analysis of the protein

Integrated bioinformatics, modelling, and gene expression analysis of the putative pentose transporter from Candida tropicalis during xylose fermentation with and without glucose addition

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