Reclassification of Clostridium aurantibutyricum Hellinger 1944 and Clostridium roseum (ex McCoy and McClung 1935) Cato et al. 1988

Schoch, Teresa; Höfele, Franziska; Odeh, Hannah; Winter, Lisa-Maria; Stöferle, Sophia; Karl, Michael M.; Bengelsdorf, Frank R.; Daniel, Rolf; Dürre, Peter

doi:10.1099/ijsem.0.005589

Cited by 2 publications

(3 citation statements)

References 23 publications

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“…The reduction of acetone to isopropanol is also known in some natural isopropanolproducing Clostridia species, e.g., C. beijerinckii and C. aurantibutyricum; the latter was recently reclassified as C. felsineum [40,41]. Clostridium species capable of isopropanol production harbor a primary/secondary alcohol dehydrogenase (SadH) specific for acetone, which is strictly NADPH-dependent [17].…”

Section: Discussionmentioning

confidence: 99%

Heterologous Production of Isopropanol Using Metabolically Engineered Acetobacterium woodii Strains

Höfele,

Schoch,

Oberlies

et al. 2023

Bioengineering

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The depletion of fossil fuel resources and the CO2 emissions coupled with petroleum-based industrial processes present a relevant issue for the whole of society. An alternative to the fossil-based production of chemicals is microbial fermentation using acetogens. Acetogenic bacteria are able to metabolize CO or CO2 (+H2) via the Wood–Ljungdahl pathway. As isopropanol is widely used in a variety of industrial branches, it is advantageous to find a fossil-independent production process. In this study, Acetobacterium woodii was employed to produce isopropanol via plasmid-based expression of the enzymes thiolase A, CoA-transferase, acetoacetate decarboxylase and secondary alcohol dehydrogenase. An examination of the enzymes originating from different organisms led to a maximum isopropanol production of 5.64 ± 1.08 mM using CO2 + H2 as the carbon and energy source. To this end, the genes thlA (encoding thiolase A) and ctfA/ctfB (encoding CoA-transferase) of Clostridium scatologenes, adc (encoding acetoacetate decarboxylase) originating from C. acetobutylicum and sadH (encoding secondary alcohol dehydrogenase) of C. beijerinckii DSM 6423 were employed. Since bottlenecks in the isopropanol production pathway are known, optimization of the strain was investigated, resulting in a 2.5-fold increase in isopropanol concentration.

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Section: Discussionmentioning

confidence: 99%

Heterologous Production of Isopropanol Using Metabolically Engineered Acetobacterium woodii Strains

Höfele,

Schoch,

Oberlies

et al. 2023

Bioengineering

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“…DNA concentrations were determined using the Qubit dsDNA HS assay kit as recommended by the manufacturer (Life Technologies). Illumina sequencing was performed as described before [5]. Illumina read data was then filtered using fastp before further analysis [6].…”

Section: Full-textmentioning

confidence: 99%

“…Therefore, cells were grown in liquid artificial seawater supplemented with 50 mM galactose. The same growth conditions were used to prepare cells for Gram-staining, which was performed as described previously [5]. Oxidase and catalase activity were tested with standard protocols.…”

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confidence: 99%

Catenovulum adriaticum sp. nov., isolated from algae in the harbour of Susak, Croatia

Schoch,

Reich,

Flaiz

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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The use of algae as feedstock for industrial purposes, such as in bioethanol production, is desirable. During a search for new agarolytic marine bacteria, a novel Gram-stain-negative, strictly aerobic, and agarolytic bacterium, designated as TS8T, was isolated from algae in the harbour of the island of Susak, Croatia. The cells were rod-shaped and motile. The G+C content of the sequenced genome was 38.6 mol%. Growth was observed at 11–37 °C, with 0.5–13 % (w/v) NaCl, and at pH 6.0–9.0. The main fatty acids were summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), and C16 : 0. The main respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Analysis of 16S rRNA gene sequences indicated that the newly isolated strain belongs to the genus Catenovulum. Based on 16S rRNA gene sequence data, strain TS8T is closely related to Catenovulum sediminis D2T (95.7 %), Catenovulum agarivorans YM01T (95.0 %), and Catenovulum maritimum Q1T (93.2 %). Digital DNA–DNA hybridization values between TS8T and the other Catenovulum strains were below 25 %. Based on genotypic, phenotypic, and phylogenetic data, strain TS8T represents a new species of the genus Catenovulum, for which the name Catenovulum adriaticum sp. nov. is proposed. The type strain is TS8T (=DSM 114830T=NCIMB 15451T).

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Reclassification of Clostridium aurantibutyricum Hellinger 1944 and Clostridium roseum (ex McCoy and McClung 1935) Cato et al. 1988

Cited by 2 publications

References 23 publications

Heterologous Production of Isopropanol Using Metabolically Engineered Acetobacterium woodii Strains

Heterologous Production of Isopropanol Using Metabolically Engineered Acetobacterium woodii Strains

Catenovulum adriaticum sp. nov., isolated from algae in the harbour of Susak, Croatia

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