Reclassification of Lactobacillus catenaformis (Eggerth 1935) Moore and Holdeman 1970 and Lactobacillus vitulinus Sharpe et al. 1973 as Eggerthia catenaformis gen. nov., comb. nov. and Kandleria vitulina gen. nov., comb. nov., respectively

Abstract

The development of molecular tools and in particular the use of 16S rRNA gene sequencing has had a profound effect on the taxonomy of many bacterial groups. Gram-positive organisms that encompass the genera Lactobacillus and Clostridium within the Firmicutes are examples of taxa that have undergone major revisions based on phylogenetic information. A consequence of these reorganizations is that a number of organisms are now recognized as being misclassified. Previous studies have demonstrated that Lactobacillus catenaformis and Lactobacillus vitulinus are phylogenetically unrelated to Lactobacillus sensu stricto, being placed within the Clostridia rRNA cluster XVII. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, it is proposed that L. catenaformis and L. vitulinus be reclassified in two new genera, named respectively Eggerthia gen. nov., with the type species Eggerthia catenaformis gen. nov., comb. nov. (type strain DSM 20559T = ATCC 25536T = CCUG 48174T = CIP 104817T = JCM 1121T) and Kandleria gen. nov., with the type species Kandleria vitulina gen. nov., comb. nov. (type strain LMG 18931T = ATCC 27783T = CCUG 32236T = DSM 20405T = JCM 1143T).

Two Different Bacterial Community Types Are Linked with the Low-Methane Emission Trait in Sheep

“…Quin’s oval appears to ferment sugars to equimolar acetate and propionate [21], which is associated with lower H 2 formation than other fermentation pathways [22]. LM2-associated ruminotype S harbored significant proportions of species characterized as lactate producers such as Sharpea azabuensis [23], Kandleria vitulina [24], [25], and Olsenella spp. ( O. uli ; [26]) and succinate producers such as Fibrobacter succinogenes , F. intestinalis [27] and Prevotella bryantii [28].…”

Evaluation of MALDI-ToF as a method for the identification of bacteria in the veterinary diagnostic laboratory

“…g -Phenotypic / biochemical methods and MALDI-ToF agreed that 21/22 of these Escherichia isolates were E. coli, although as for 16S rDNA sequence, MALDI-ToF can not distinguish between Escherichia and Shigella.. For the remaining isolate the identity was Escherichia fergusonii by MALDI-ToF but Mannheimia haemolytica by phenotypic / biochemical tests.h -This isolate matched had 16S rDNA sequence < 99% homology to the type strain of Gallibacterium anatis although identical to some field isolates described as this species. Both MALDI-ToF and phenotypic / biochemically identifications were Gallibacterium anatis, although for MALDI-ToF the confidence level was to genera only.i -MALDI-ToF identified this isolate as Lactobacillis vitulinus which has been renamed as Kandleria vitulina(Salvetti et al, 2011).j -These isolates mostly showed best 16S rDNA sequence homology to the type strain of Klebsiella pneumoniae subsp. Pneumoniae, but strong matches to other species/subspecies such as Klebsiella pneumoniae subsp.…”

DFAST and DAGA: web-based integrated genome annotation tools and resources

“…The genus Sharpea was not included even though it is classified in the family Lactobacillaceae . Sharpea azabuensis , the only member of this genus, was initially described as a species related to Lactobacillus catenaformis , but L. catenaformis was later reclassified as Eggerthia catenaformis , and it is no longer a member of Lactobacillaceae [34, 35]. As the number of available genomes is increasing rapidly, we plan to update the database regularly and to expand the scope of the database to other taxonomic groups.…”