Reclassification of Sabulilitoribacter multivorans and Sabulilitoribacter arenilitoris as Flaviramulus multivorans comb. nov. and Wocania arenilitoris comb. nov., respectively, based on genome sequence analysis

Abstract

Rapid advancements in DNA sequencing technologies are providing new approaches for bacterial taxonomy. The genus Sabulilitoribacter is a member of the family Flavobacteriaceae , which consists of more than 150 genera. In this study, genome sequence analysis was conducted to revisit the taxonomic status of Sabulilitoribacter arenilitoris and Sabulilitoribacter multivorans , the only two species of this genus. Genome sequence based phylogeny analysis showed that the genus Sabulilitoribacter was non-monophyletic: S. multivorans , the type species of genus Sabulilitoribacter , was clustered with the type species of the genus Flaviramulus , whereas S. arenilitoris formed a robust cluster with the only two species of the genus Wocania . The values of average amino acid identity, genome-wide average nucleotide identity, alignment fractions and some phenotypic features showed that S. multivorans was more closely related with the type species of the genus Flaviramulus than with S. arenilitoris , and S. arenilitoris was more closely related with the only two species of the genus Wocania than with S. multivorans . Based on these results, we consequently propose that S. multivorans and S. arenilitoris should be reclassified as Flaviramulus multivorans comb. nov. and Wocania arenilitoris comb. nov. respectively.

Parasedimentitalea denitrificans sp. nov., a novel denitrifying bacteria isolated from the Yellow Sea and transfer of Zongyanglinia huanghaiensis and Zongyanglinia marina to the genus Parasedimentitalea as Parasedimentitalea huanghaiensis comb. nov. and Parasedimentitalea maritima nom. nov.

“…In order to comprehensively analyse the taxonomic status of CY04 T , genome-based phylogenetic analysis was conducted based on two set of sequences: the amino acid sequence of single-copy orthologous clusters (OCs) consisting of 1176 proteins and the amino acid sequence of 120 bacterial single-copy conserved marker proteins. Reconstruction of the phylogenomic trees based on sequences of OCs were performed as described by Liu et al [18]. Reconstruction of the phylogenomic tree based on 120 bacterial single-copy conserved marker proteins sequence was performed with GTDB-Tk 1.3.0 [19].…”