Recognition and receptor-mediated uptake of a lysosomal enzyme, α-l-iduronidase, by cultured human fibroblasts

Sando, Gloria N.; Neufeld, Elizabeth F.

doi:10.1016/0092-8674(77)90262-8

Cited by 348 publications

(155 citation statements)

References 17 publications

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“…2). Third, incubation of cells with HRP in the presence of saccharides known to inhibit receptor-mediated pinocytosis (8,16,20) had no effect on the uptake of HRP, a glycoprotein (Table 1) . Fourth, no cell surface binding of HRP was detected by electron microscopic cytochemistry after exposure of cells to peroxidase at either 4°C or 37°C (Fig.…”

Section: Mode Of Hrp Internalizationmentioning

confidence: 99%

Exocytosis of pinocytic contents by Chinese hamster ovary cells.

Adams¹,

Maurey²,

Storrie³

1982

The Journal of Cell Biology

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The extent of exocytosis of pinocytic vesicle contents was studied in suspensioncultured Chinese hamster ovary (CHO) cells using horseradish peroxidase (HRP) as a pinocytic content marker. HRP was shown to be internalized via fluid-phase pinocytosis in CHO cells.After an HRP pulse of 2 .5-10 min a rapid decrease of 30-50% in cell-associated HRP activity was observed within 10-20 min at 37°C. During this time the loss of cell-associated HRP was accompanied by an equivalent increase in extracellular HRP. After this rapid exocytosis of HRP, the remaining peroxidase activity decreased with a t1/2 of 6-8 h, the known lysosomal half-life of HRP. In pulse-chase experiments HRP was chased into a nonexocytic compartment. Based on cell fractionation and electron microscopic experiments, this nonexocytic compartment was identified as a lysosome and the compartment from which exocytosis occurs as a pinosome . The occurrence of pinocytic content exocytosis in cultured fibroblasts suggests that exocytosis of pinocytic vesicle contents is a general phenomenon .

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Section: Mode Of Hrp Internalizationmentioning

confidence: 99%

Exocytosis of pinocytic contents by Chinese hamster ovary cells.

Adams¹,

Maurey²,

Storrie³

1982

The Journal of Cell Biology

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“…It was first found by Neufeld and her associates (90,191) that strains of genetically deficient fibroblasts (I cells) from patients with mucolipidosis II secrete lysosomal hydrolases into the medium rather than incorporating them into lysosomes . These enzymes, although enzymatically active, were found to lack a recognition marker for uptake by normal fibroblasts, whereas lysosomal hydrolases from normal cells could be taken up by a specific and saturatable process by both normal and I cell fibroblasts .…”

Section: Sorting-out Processes Must Follow Cotranslational Insertion mentioning

confidence: 99%

Mechanisms for the incorporation of proteins in membranes and organelles.

Sabatini¹,

Kreibich²,

Morimoto³

et al. 1982

The Journal of Cell Biology

869

281

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“…Human diploid fibroblasts were obtained from young healthy volunteers by skin biopsy and grown in Dulbecco's minimal essential medium (DMEM, Gibco Wiesbaden, FRG) supplemented with 10% fetal calf serum (Gibco), 2% pyruvate, 2mM glutamine, and non-essential amino acids [20].…”

Section: Cell Culturementioning

confidence: 99%

Induction of surface IgG receptors in cytomegalovirus‐infected human fibroblasts

Frey

Einsfelder

1984

European Journal of Biochemistry

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Binding studies on diploid human fibroblasts with human immunoglobulin G (IgG) demonstrate the existence of a specific receptor for this class of immunoglobulin. The receptor preferentially binds aggregated human IgG and recognizes these complexes via the Fc portion of the molecule. Cytomegalovirus infection of diploid human fibroblasts results in a more than 100-fold increase in the number of IgG-receptors present on the cell surface. We have previously demonstrated that fibroblasts express a receptor with high specificity for human IgG (J. Frey and E. G. Afting unpublished results). It has recently been shown that peripheral tissues, e. g. skin and muscle, account for the highest proportion of IgG catabolism [19]. These tissues contain a high proportion of fibroblasts. The following study was performed in order to investigate IgG receptors on human fibroblasts after virus infection. Here we describe the induction of an Fc-receptor on human fibroblasts following infection with cytomegalovirus (CMV). The induced receptor shows a high affinity for the aggregated Fc domain of human IgG. MATERIALS AND METHODS Cell cultureHuman diploid fibroblasts were obtained from young healthy volunteers by skin biopsy and grown in Dulbecco's minimal essential medium (DMEM, Gibco Wiesbaden, FRG) supplemented with 10% fetal calf serum (Gibco), 2% pyruvate, 2mM glutamine, and non-essential amino acids [20].The cells were maintained at 37 "C in 10 % COz. CMV were propagated in human fibroblasts [21]. For binding studies using infected cells, subconfluent or confluent cells growing in 6 cm petri-dishes were washed twice with serum-free DMEM and subsequently infected with 4 -5 TCIDSO of CMV per cell in 3 ml serum free mcdium for 2 h. After two washing steps the cells were further kept in complete medium. The cell viability was determined by trypan blue exclusion [22].

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Recognition and receptor-mediated uptake of a lysosomal enzyme, α-l-iduronidase, by cultured human fibroblasts

Cited by 348 publications

References 17 publications

Exocytosis of pinocytic contents by Chinese hamster ovary cells.

Exocytosis of pinocytic contents by Chinese hamster ovary cells.

Mechanisms for the incorporation of proteins in membranes and organelles.

Induction of surface IgG receptors in cytomegalovirus‐infected human fibroblasts

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