Recognition behavior of chiral nanocomposites toward biomolecules and its application in electrochemical immunoassay

Abstract

The novel nanocomposites were obtained by covalently linking arginine enantiomers with the sidewall of multi-walled carbon nanotubes, and utilized as sensing materials for biomolecular recognition in electrochemical immunoassay. They were characterized by X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and circular dichroism spectroscopy (CD). Nano-gold and Prussian Blue were employed to amplify the analytical signal responses. Prostate specific antibody/antigen and carcinoembryonic antibody/antigen were used as model systems. The results showed that the different configurations of nanocomposites could readily facilitate chiral recognition of antibodies, and D-nanocomposites displayed a larger signal.arginine, multi-walled carbon nanotubes, chiral nanocomposites, recognition, electrochemical immunoassay

Chiral Nanoparticles Force Neural Stem Cell Differentiation to Alleviate Alzheimer's Disease

“…[21,22] Chiral NPs with specific optical signal responses are widely used as biological probes for highly sensitive detection of biomolecules, catalysis, and biosensors. [23][24][25][26][27][28][29][30] In addition, chiral NPs have better biocompatibility, can effectively reduce the toxic effect on cells, and have higher safety when interacting with biomolecules. [24,[31][32][33][34] They can also enhance the uptake capacity of cells, have stronger sustained release ability, and specific enantiomer selectivity, which can improve the therapeutic effect in tumors, neurodegenerative diseases, and other diseases.…”

Chiral glutamic acid functionalized graphene: preparation and application

“…While still early in its evolution and application, chiral materials are at the point of moving from understanding chirality in the nanoscale, such as functional self-assembly, 1 enantioselective catalysis 2 and optical devices. [5][6][7] Graphene, emerging as the monolayer of a honeycomb lattice packed with carbon atoms, has received signicant attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass production). 4 Synthesis of such chiral inorganic-organic composite materials has been widely reported due to their unique properties and promising potential for a wide range of applications.…”

“…4 Synthesis of such chiral inorganic-organic composite materials has been widely reported due to their unique properties and promising potential for a wide range of applications. [5][6][7] Graphene, emerging as the monolayer of a honeycomb lattice packed with carbon atoms, has received signicant attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass production). 8 Recently, much effort has been made to prepare functionalized graphene nanocomposites by incorporating organic moieties onto oxygen-containing groups (e.g., hydroxyl, carboxyl, and epoxy) of graphene oxide.…”

Study on Chiral Nanocomposite for Recognition of DOPA Enantiomers via Square Wave Voltammetry

“…2 Among various achiral nanomaterials, multi-walled carbon nanotubes (MWCNTs) are widely applied to prepare chiral nanocomposites due to their narrow size distribution, large specific surface area, good chemical stability and excellent electrochemical properties. [3][4][5][6][7][8][9][10][11] Unfortunately, the application of pristine MWCNTs have been restricted for the easy agglomeration and poor dispersibility. In order to obtain better dispersity and introduce more binding sites, a series of methods have been adopted for the surface functionalization of MWCNTs, such as aggressive oxidation treatment with HNO 3 or a HNO 3 -H 2 SO 4 mixture, 12,13 wrapping with polymer, 14,15 grafting of ionic liquid, 16,17 modification by π-stacking, 18 etc.…”