Recognition mechanisms in the synthesis of animal virus DNA

Hay, Ronald T.; Russell, W. C.

doi:10.1042/bj2580003

Cited by 56 publications

(31 citation statements)

References 182 publications

(152 reference statements)

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“…Located within the ITRs are the cis-acting DNA sequences which define ori, the origin of DNA replication (1). Covalently attached to each 5'-end is a terminal protein (TP) which is likely to be an additional cis-acting component of ori (2).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the adenovirus preterminal protein and its interaction with the POU homeodomain of NFIII (Oct-1)

Botting

Hay

1999

Nucleic Acids Research

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Section: Introductionmentioning

confidence: 99%

Characterisation of the adenovirus preterminal protein and its interaction with the POU homeodomain of NFIII (Oct-1)

Botting

Hay

1999

Nucleic Acids Research

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“…Ad DNA replication can be studied in vitro (10)(11)(12)(13)(14)(15), enabling us to study the mechanism of inhibition of (S)-HPMPApp in more detail. Moreover, since we obtained evidence that the Ad DNA polymerase is the prime target of the drug, we investigated the effect of (S)-HPMPApp on highly purified pTP-pol using artificial templates.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to the pTP the initiation reaction requires the Ad-encoded DNA polymerase (pol) of 140 kDa and the cellular proteins nuclear factor I and Im (NFI and NFIII), while the 72 kDa Ad DNA binding protein (DBP) stimulates the reaction considerably. Elongation to full length Ad DNA chains requires pol, DBP, and another cellular protein, nuclear factor II with topoisomerase I activity (for reviews see [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Mechanism of inhibition of adenovirus DNA replication by the acyclic nucleoside triphosphate analogue (S)-HPMPApp: influence of the adenovirus DNA binding protein

Mul

Miltenburg²,

Clercq

et al. 1989

Nucl Acids Res

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The acyclic adenosine analogue (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine t(S)-HPMPAj is a potent and selective inhibitor of adenovirus (Ad) replication in cell culture. We studied the mechanism of inhibition using a reconstituted in vitro DNA replication system. The diphosphoryl derivative (S)-HPMPApp, but not (S)-HPMPA, inhibited the DNA replication of origin containing fragments strongly. The inhibitory effect was exerted at the level of elongation, while initiation was resistant to the drug. Remarkably, the elongation of short strands was only slightly impaired, while inhibition was maximal upon synthesis of long DNA fragments. (S)-HPMPApp appeared to be competitive with dATP, suggesting that the Ad DNA polymerase is the prime target for the drug. We purified the Ad DNA polymerase in complex to the precursor terminal protein to homogeneity from cells infected with overproducing recombinant vaccinia viruses. Employing gapped DNA or poly(dT).oligo(dA) templates, only a weak inhibition was observed. However, inhibition was strongly enhanced in the presence of the adenovirus DNA binding protein (DBP). We interpret this to mean that the increased processivity of the polymerization reaction in the presence of DBP leads to increased drug sensitivity.

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“…The dissection and reconstitution of the cell-free DNA replication system using the Ad DNA terminal protein complex (Ad DNAprot) have revealed the detailed mechanism of Ad genome replication (1)(2)(3). The Ad genome is a linear DNA of '36 kbp that contains 55-kDa terminal proteins covalently attached to its 5' ends.…”

mentioning

confidence: 99%

Replication factor encoded by a putative oncogene, set, associated with myeloid leukemogenesis.

Nagata

Kawase

Handa

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

170

180

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DNA replication and transcription of adenovirus (Ad) have been studied extensively as a model eukaryotic system. The dissection and reconstitution of the cell-free DNA replication system using the Ad DNA terminal protein complex (Ad DNAprot) have revealed the detailed mechanism of Ad genome replication (1-3). The Ad genome is a linear DNA of '36 kbp that contains 55-kDa terminal proteins covalently attached to its 5' ends. Replication of the Ad DNA-prot initiates by a protein-priming mechanism in which the 5' terminal nucleotide of the nascent DNA, dCMP, is linked to the 80-kDa

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Recognition mechanisms in the synthesis of animal virus DNA

Cited by 56 publications

References 182 publications

Characterisation of the adenovirus preterminal protein and its interaction with the POU homeodomain of NFIII (Oct-1)

Characterisation of the adenovirus preterminal protein and its interaction with the POU homeodomain of NFIII (Oct-1)

Mechanism of inhibition of adenovirus DNA replication by the acyclic nucleoside triphosphate analogue (S)-HPMPApp: influence of the adenovirus DNA binding protein

Replication factor encoded by a putative oncogene, set, associated with myeloid leukemogenesis.

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