Recognition of HIV-derived B and T cell epitopes displayed on filamentous phages

Berardinis, Piergiuseppe De; D’Apice, Luciana; Prisco, Antonella; Ombra, Maria Neve; Barba, Pasquale; Pozzo, Giovanna Del; Petukhov, S. A.; Malik, Pratap; Perham, Richard N.; Guardiola, John

doi:10.1016/s0264-410x(98)00377-6

Cited by 40 publications

(40 citation statements)

References 19 publications

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“…We have described the ability of fd virions, engineered to display multiple copies of foreign peptide-surface epitopes, to evoke both B cell-and T cell-mediated immune responses (24). Antigenic peptides can be expressed on the capsid of the filamentous bacteriophage fd, in the exposed Nterminal region of the 2700 copies of the major coat protein pVIII, and elicit the production of high titers of Abs and/or the generation of Th cell responses (24 -28).…”

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confidence: 99%

The Use of Filamentous Bacteriophagefdto Deliver MAGE-A10 or MAGE-A3 HLA-A2-Restricted Peptides and to Induce Strong Antitumor CTL Responses

Sartorius

Pisu

D’Apice

et al. 2008

The Journal of Immunology

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Delivery of tumor-associated Ag-derived peptides in a high immunogenic form represents one of the key issues for effective peptide-based cancer vaccine development. We report herein the ability of nonpathogenic filamentous bacteriophage fd virions to deliver HLA-A2-restricted MAGE-A10254–262- or MAGE-A3271–279-derived peptides and to elicit potent specific CTL responses in vitro and in vivo. Interestingly, human anti-MAGE-A3271–279-specific CTLs were able to kill human MAGE-A3+ tumor cells, even if these cells naturally express a low amount of MAGE-A3271–279 peptide-HLA epitope surface complexes and are usually not recognized by CTLs generated by conventional stimulation procedures. MAGE-A3271–279-specific/CD8+ CTL clones were isolated from in vitro cultures, and their high avidity for Ag recognition was assessed. Moreover, in vivo tumor protection assay showed that vaccination of humanized HHD (HLA-A2.1+/H2-Db+) transgenic mice with phage particles expressing MAGE-A3271–279-derived peptides hampered tumor growth. Overall, these data indicate that engineered filamentous bacteriophage virions increase substantially the immunogenicity of delivered tumor-associated Ag-derived peptides, thus representing a novel powerful system for the development of effective peptide-based cancer vaccines.

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confidence: 99%

The Use of Filamentous Bacteriophagefdto Deliver MAGE-A10 or MAGE-A3 HLA-A2-Restricted Peptides and to Induce Strong Antitumor CTL Responses

Sartorius

Pisu

D’Apice

et al. 2008

The Journal of Immunology

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“…8,9 Filamentous phage particles are an excellent antigen delivery system for production of antibodies 10 and mice immunized with recombinant phage elicit cellular immunity. 11 Recently we reported that the injection of hybrid phage particles into BALB/c mice efficiently primed MHC class I-restricted hepatitis B virus specific CTL responses. 9 Studies examining the protective potential of recombinant filamentous phage displayed CTL epitopes have not been reported.…”

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confidence: 99%

Phage display particles expressing tumor‐specific antigens induce preventive and therapeutic anti‐tumor immunity in murine p815 model

Wan

Bian

et al. 2002

Intl Journal of Cancer

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The efficacy of phage display particles expressing tumor antigen P1A35-43 in inducing protective and therapeutic anti-tumor immune responses were studied. A protective immune response against a lethal progressive P815 mastocytoma tumor cell challenge was established after subcutaneous injection of phage display particles. Furthermore, the vaccine suppressed growth of pre-existing tumors. Immunization with the hybrid phage particles elicited P1A 35-43 specific CTL responses and a Th1-dominated immune response with phage particle-specific secretion of IFN-␥ but not IL-4. Our results indicate that phage display particles might be a useful vaccine form for tumor-associated antigen epitopes in tumor immunotherapy.

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“…A disadvantage of using phage is the relatively weak T-cell help they provide. This issue may be addressed by modifying phage to include immunodominant foreign TCEs with known properties [39,40,59], either by direct fusion to the phage coat proteins or chemical conjugation to pVIII (our unpublished data).…”

Section: Modification Of Phage Carriers For Optimizing Immune Responsesmentioning

confidence: 99%

“…Phage have also been shown to elicit CD4 T-cell help, form immunological memory [24] and are well-documented as immunogenic carriers for recombinant peptides [25][26][27][28][29][30][31][32][33][34][35] (for reviews see [36][37][38]). Furthermore, phage can be engineered to enhance the immune response, for example, by the addition of foreign CD4 T-cell epitopes to pIII or pVIII [39,40].…”

Section: Introductionmentioning

confidence: 99%

Filamentous phage as an immunogenic carrier to elicit focused antibody responses against a synthetic peptide

Houten

Zwick

Menéndez

et al. 2006

Vaccine

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Filamentous bacteriophage are widely used as immunogenic carriers for "phage-displayed" recombinant peptides. Here we report that they are an effective immunogenic carrier for synthetic peptides. The f1.K phage was engineered to have an additional Lys residue near the N-terminus of the major coat protein, pVIII, so as to enhance access to chemical cross-linking agents. The dimeric synthetic peptide, B2

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Recognition of HIV-derived B and T cell epitopes displayed on filamentous phages

Cited by 40 publications

References 19 publications

The Use of Filamentous Bacteriophagefdto Deliver MAGE-A10 or MAGE-A3 HLA-A2-Restricted Peptides and to Induce Strong Antitumor CTL Responses

The Use of Filamentous Bacteriophagefdto Deliver MAGE-A10 or MAGE-A3 HLA-A2-Restricted Peptides and to Induce Strong Antitumor CTL Responses

Phage display particles expressing tumor‐specific antigens induce preventive and therapeutic anti‐tumor immunity in murine p815 model

Filamentous phage as an immunogenic carrier to elicit focused antibody responses against a synthetic peptide

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