Recognition of methylated DNA by TAL effectors

Deng, Dong; Yin, Ping; Yan, Chuangye; Pan, Xiaojing; Gong, Xinqi; Qi, Shiqian; Xie, Tian; Mahfouz, Magdy M.; Zhu, Jian‐Kang; Yan, Nieng; Shi, Yigong

doi:10.1038/cr.2012.127

Cited by 106 publications

(86 citation statements)

References 11 publications

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“…Conventional TALENs cannot cleave DNA containing 5-methylcytosine but methylated cytosine is indistinguishable from thymidine in the major groove. Therefore, the repeat that recognizes cytosine can be replaced with a repeat which recognizes thymidine, generating TALENs that can cleave methylated DNA albeit at the expense of target specificity (Deng et al , 2012, Valton et al , 2012.…”

Section: Advantages Of the Crispr/cas9 Systemmentioning

confidence: 99%

The CRISPR/Cas9 system for plant genome editing and beyond

Bortesi

Fischer

2015

Biotechnology Advances

1,020

593

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Targeted genome editing using artificial nucleases has the potential to accelerate basic research as well as plant breeding by providing the means to modify genomes rapidly in a precise and predictable manner. Here we describe the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a recently developed tool for the introduction of site-specific double-stranded DNA breaks. We highlight the strengths and weaknesses of this technology compared with two well-established genome editing platforms: zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). We summarize recent results obtained in plants using CRISPR/Cas9 technology, discuss possible applications in plant breeding and consider potential future developments.

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Section: Advantages Of the Crispr/cas9 Systemmentioning

confidence: 99%

The CRISPR/Cas9 system for plant genome editing and beyond

Bortesi

Fischer

2015

Biotechnology Advances

1,020

593

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“…However, the use of TALEs in particular can be advantageous when a particular target area lacks a PAM, since TALEs can be programmed to target any sequence. TALEs are able to distinguish between methylcytosine (mC) and cytosine (C), which may be advantageous or disadvantageous, depending on the situation (52,53,63). The methylation status of the targeted site must be taken into account when designing TALEs, which is not the case for Cas9 targeting.…”

Section: Crispra Versus Previous Activation Methodsmentioning

confidence: 99%

The New State of the Art: Cas9 for Gene Activation and Repression

Russa

2015

Molecular and Cellular Biology

206

150

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CRISPR-Cas9 technology has rapidly changed the landscape for how biologists and bioengineers study and manipulate the genome. Derived from the bacterial adaptive immune system, CRISPR-Cas9 has been coopted and repurposed for a variety of new functions, including the activation or repression of gene expression (termed CRISPRa or CRISPRi, respectively). This represents an exciting alternative to previously used repression or activation technologies such as RNA interference (RNAi) or the use of gene overexpression vectors. We have only just begun exploring the possibilities that CRISPR technology offers for gene regulation and the control of cell identity and behavior. In this review, we describe the recent advances of CRISPR-Cas9 technology for gene regulation and outline advantages and disadvantages of CRISPRa and CRISPRi (CRISPRa/i) relative to alternative technologies.

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“…In addition, 5-mC is available in CpG islands in regulatory regions of genes such as promoter (Maunakea et al 2010). Valton et al (2012) and Deng et al (2012b) independently found that NG and N* RVDs (an asterisk indicates a deletion at residue 13 in the repeat unit) recognize 5-mC with a high efficiency.…”

Section: New Approaches For Reducing Limitations In Using Of Talens Tmentioning

confidence: 97%

Technology developments in biological tools for targeted genome surgery

Teimourian

Abdollahzadeh

2014

Biotechnol Lett

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Different biological tools for targeted genome engineering have recently appeared and these include tools like meganucleases, zinc-finger nucleases and newer technologies including TALENs and CRISPR/Cas systems. transcription activator-like effector nucleases (TALENs) have greatly improved genome editing efficiency by making site-specific DNA double-strand breaks. Several studies have shown the prominence of TALENs in comparison to the meganucleases and zinc-finger nucleases. The most important feature of TALENs that makes them suitable tools for targeted genome editing is the modularity of central repeat domains, meaning that they can be designed to recognize any desirable DNA sequence. In this review, we present a comprehensive and concise description of TALENs technology developments for targeted genome surgery with to the point description and comparison of other tools.

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Recognition of methylated DNA by TAL effectors

Cited by 106 publications

References 11 publications

The CRISPR/Cas9 system for plant genome editing and beyond

The CRISPR/Cas9 system for plant genome editing and beyond

The New State of the Art: Cas9 for Gene Activation and Repression

Technology developments in biological tools for targeted genome surgery

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