Recognition of the three deduced probable HLA haplotypes that are associated with HLA-C∗16:04:01 (A∗33:03-B∗44:02-C∗16:04:01-DRB1∗11:04:01 and A∗24-B∗44:02-C∗16:04:01-DRB1∗11:04) and HLA-B∗15:109 (A∗11-B∗15:109-DRB1∗04) in Taiwanese unrelated hematopoietic stem cell donors

Abstract

a b s t r a c tObjective: HLA-C*16:04:01 and HLA-B*15:109 are two uncommon alleles at the HLA-C locus and HLA-B locus, respectively. The objective of this study is to report the deduced probable human leukocyte antigen (HLA) haplotypes associated with HLA-C*16:04:01 and HLA-B*15:109 among Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A sequence-based typing method was employed to confirm the two low incidence alleles observed. Polymerase chain reaction was performed to amplify exons 2 and 3 of the HLA-A, and HLA-B loci with group-specific primer sets. Amplicons were sequenced using a BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocols. Results: The DNA sequence of C*16:04:01 is identical to that of C*16:01:01 in exons 2 and 3, except for two nucleotide substitutions at residues 538 (C->T) and 539 (A->G), which results in a single amino acid replacement at position 156 (glutamine->tryptophan). We deduced two probable HLA haplotypes that are found in association with C