Recognition System for Dietary Fatty Acids in the Rat Small Intestine

Shintani, Takafumi; Takahashi, Nobuyuki; Fushiki, Tohru; Kotera, Jun; Sugimoto, Etsuro

doi:10.1271/bbb.59.1428

Cited by 22 publications

(11 citation statements)

References 5 publications

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“…These results suggest that the carboxyl group also plays a role in fatty acid uptake. However, compared to our previous study on calcium mobilization by lipids (15,19), carboxyl group had a weak effect on fatty acid uptake. The fatty acid recognition mechanism by signal transduction pathways and absorption may not be the same.…”

Section: Discussioncontrasting

confidence: 97%

“…We have utilized IEC-6 cells as an adequate in vitro system of intestinal epithelial cells. Previously, we found that fatty acids activate the mobilization of 45 Ca 2+ , which might be concerned with signaling pathway by dietary fat in IEC-6 cells (19) as well as dispersed rat intestinal cells (15). This effect was inhibited by the presence of a fatty acid methyl ester and glutamic acid.…”

Section: Discussionmentioning

confidence: 88%

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Influence of fatty alcohol and other fatty acid derivatives on fatty acid uptake into rat intestinal epithelial cells

Murota¹,

Matsui²,

Kawada³

et al. 2001

Lipids

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We investigated the influence of various substrates on the uptake of long-chain fatty acid into IEC-6, rat intestinal epithelial cell line. The uptake of [3H]oleic acid into IEC-6 cells was a saturable function of the oleic acid concentration. Long-chain fatty acids significantly inhibited the oleic acid uptake into IEC-6 cells and shorter-chain fatty acids had little or no effect. Various fatty acid esters suppressed the oleic acid uptake into IEC-6. Fatty alcohols also inhibited oleic acid uptake into IEC-6 and the length of the carbon chain played an important role. These results suggest that long-chain fatty acid uptake was inhibited by the substrates which had a structure similar to long-chain fatty acids, especially those with a long carbon chain. At least two molecules, fatty acid translocase and fatty acid transport protein type 4, which are considered to be involved in the long-chain fatty acid transport into the cell, were expressed on IEC-6 cells, supporting the existence of the carrier-mediated system of long-chain fatty acid transport on IEC-6 cells.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussionmentioning

confidence: 88%

Influence of fatty alcohol and other fatty acid derivatives on fatty acid uptake into rat intestinal epithelial cells

Murota¹,

Matsui²,

Kawada³

et al. 2001

Lipids

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“…Shintani et al 19) reported that there are receptors for food components such as fatty acids and amino acids on the surfaces of intestinal cells. The receptors trigger gastrointestinal hormone release after Ca influx into the cells when they recognize any signals from food components; and thereby, the secretion of digestive juices such as bile juice is accelerated.…”

Section: Discussionmentioning

confidence: 99%

Promotive Effects of the Dietary Organic Germanium Poly-trans-[(2-carboxyethyl) germasesquioxane] (Ge-132) on the Secretion and Antioxidative Activity of Bile in Rodents

Nakamura

Nagura

Akiba

et al. 2010

JOURNAL OF HEALTH SCIENCE

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Poly-trans-[(2-carboxyethyl) germasesquioxane] (Ge-132) is the most common organic germanium compound. This compound has many physiological effects, which are mediated via the modulation of immune-system activation. The intake of dietary Ge-132 causes fecal color changes in humans, and we studied the mechanism of this in a rodent model. Male Wistar rats were given a diet containing 0.05% Ge-132 for two weeks and were compared with rats given a germanium free diet. The color of their feces and cecal contents changed from grayish-green to yellow in rats fed with Ge-132. The concentrations of stercobilin, a major fecal pigment, and total bile acids in cecal contents, were significantly increased by dietary Ge-132. Stercobilin is a metabolite of the bile pigment bilirubin. These results were produced by increases in bile components, such as bilirubin and bile acids, and showed that dietary Ge-132 promotes bile secretion into the intestine. Next, we administered Ge-132 (per os) to male rats at 50 mg/kg body weight per day for four days to reveal its effect on bile (and particularly on bilirubin). Bile juice samples were collected, and their bilirubin content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were analyzed. The bilirubin level in the bile was significantly increased by the administration of Ge-132, and the DPPH radical scavenging activity of bile was also significantly increased. As the increases in these two factors were correlated, we supposed that the anti-oxidative properties of the bile of rats fed with Ge-132 were due to bilirubin glucuronides. Oral intake of Ge-132 also increased the mRNA expression of uridine diphosphate glucuronosyltransferase 1a1 (Ugt1a1) and bile acid coenzyme A: amino acid N-acyltransferase (Baat), which encode bile component conjugating enzymes for secretion. The acceleration of bile pigment secretion into the intestine as well as increases in the anti-oxidant activity of bilirubin was induced by oral intake of dietary Ge-132. It is suggested that the antioxidative effect of bile against oxidative stress occurs through radical trapping.

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“…We considered that the mechanism of the inhibition effect of oleyl alcohol on long-chain fatty acid absorption in our "in vivo" experiments might involve, at least in part, the competitive inhibition of the uptake into the enterocyte by a carrier-mediated system. We have pre viously demonstrated that molecule(s) that recognize long-chain fatty acid specifically exist in the cellular membrane of rat isolated enterocytes (6) and IEC-6, rat intestinal epithelial cell line (7), and this recognition was inhibited by fatty acid derivatives. These results suggest that oleyl alcohol inhibits the gas trointestinal absorption of long-chain fatty acids in vivo.…”

Section: Discussionmentioning

confidence: 99%