2016
DOI: 10.1134/s1607672916010038
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Recombinant 1F9 spidroin microgels for murine full-thickness wound repairing

Abstract: The study of the stimulating effect of the microgels (MGs) based on recombinant 1F9 spidroin on the regeneration of the deep skin wound in mice was carried out. The use of spidroin MGs was shown to increase significantly the quality of healing compared to the control. The introduction of the MG in the wound edges led to recovery of all the structural elements of the skin: the epidermis, the dermis, including vascular and nervous network, in the periphery of the wound underlying muscles, and skin appendages (se… Show more

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Cited by 7 publications
(8 citation statements)
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“…However, whereas in the latter host 70–80% of MaSp2 was in the water-insoluble cellular fraction, in P. pastoris 60% was in the soluble fraction. The proteins are being developed into matrices for tissue engineering and drug delivery (Agapov et al, 2009; Moisenovich et al, 2016; Moisenovich et al, 2011; Nosenko et al, 2018).…”
Section: Protein Polymers Produced In P Pastorismentioning
confidence: 99%
“…However, whereas in the latter host 70–80% of MaSp2 was in the water-insoluble cellular fraction, in P. pastoris 60% was in the soluble fraction. The proteins are being developed into matrices for tissue engineering and drug delivery (Agapov et al, 2009; Moisenovich et al, 2016; Moisenovich et al, 2011; Nosenko et al, 2018).…”
Section: Protein Polymers Produced In P Pastorismentioning
confidence: 99%
“…The skin of A. caliginosa earthworms that received the spidroin gel did not show any considerable differences from that of control group (Figure e,f & a,b, respectively). Wound healing involves growth of capillaries, fibroblasts and collagen, forming granulation tissue (Desmoulière, Chaponnier, & Gabbiani, ; Moisenovich et al, ). In the present study, we observed more fibroblasts and capillaries in skin of earthworms that received the spidroin gel compared with those that received the plain gel at the third day.…”
Section: Resultsmentioning
confidence: 99%
“…Spidroin is very large protein (>300 kDa), typically classified into spidroin‐I and spidroin‐II due to differences in proline and tyrosine amino acids content; these two are responsible for silk strength and elasticity, respectively (Butler, ). Recombinant spidroin was reported to promote cell proliferation and angiogenesis in wounds (Moisenovich et al, ; Moisenovich et al, ; Veith, Henderson, Spencer, Sligar, & Baker, ). Additionally, spidroin has a potential medical application due to a fascinating combination of wide temperature‐ and pH‐stability, biodegradability, biocompatibility, nontoxicity, and nonimmunogenicity (Brown, Whaite, MacLeod, Macdonald, & Rosei, ; Butler, ).…”
Section: Introductionmentioning
confidence: 99%
“…It was shown [3] that the implantation of a scaffold made from decellularized submucosa stimulates angiogenesis, the growth of epithelial and connective tissue and smooth muscle fibers, and the formation of all layers of the intestinal wall 12 weeks after implantation. A promising materials for the creation of bioartificial tissues is silk [4,5]. Bombyx mori silk fibroin has a number of unique properties, such as mechanical strength, biocompatibility, bioresorption, and availability.…”
mentioning
confidence: 99%