Recombinant Allergens for Diagnosis of Cockroach Allergy

Arruda, L. Karla; Barbosa, Michelle; Santos, Antônio Cardozo dos; Moreno, Adriana S.; Chapman, Martin D.; Pomés, Anna

doi:10.1007/s11882-014-0428-6

Cited by 35 publications

(29 citation statements)

References 99 publications

(101 reference statements)

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“…However, other allergen sources such as cockroach do not have a dominant allergen. Early studies revealed that Bla g 1, Bla g 2, Bla g 4, and Bla g 5 identify ~95 % of cockroach-allergic patients in the USA [103]. As more allergens have been identified around the world, different patterns of IgE sensitization to cockroach have been found in other populations.…”

Section: Introductionmentioning

confidence: 99%

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Indoor Allergens and Allergic Respiratory Disease

Pomés

Chapman

Wünschmann

2016

Curr Allergy Asthma Rep

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Purpose of review The purpose of this review is to evaluate the most recent findings on indoor allergens and their impact on allergic diseases. Recent findings Indoor allergens are present inside buildings (home, work environment, school), and given the chronic nature of the exposures, indoor allergies tend to be associated with the development of asthma. The most common indoor allergens are derived from dust mites, cockroaches, mammals (including wild rodents and pets), and fungi. The advent of molecular biology and proteomics has led to the identification, cloning, and expression of new indoor allergens, which have facilitated research to elucidate their role in allergic diseases. This review is an update on new allergens and their molecular features, together with the most recent reports on their avoidance for allergy prevention and their use for diagnosis and treatment. Summary Research progress on indoor allergens will result in the development of new diagnostic tools and design of coherent strategies for immunotherapy.

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Section: Introductionmentioning

confidence: 99%

“…IgE reactivity to cockroach tropomyosin was found to be dominant in Brazil [27]. Overall, a cocktail of five cockroach allergens from groups 1, 2, 4, 5, and 7 would allow to diagnose 50–65 % of patients worldwide [103]. …”

Section: Introductionmentioning

confidence: 99%

Indoor Allergens and Allergic Respiratory Disease

Pomés

Chapman

Wünschmann

2016

Curr Allergy Asthma Rep

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“…These recombinant allergens can not only serve as accurate diagnostic agents, 32, 33 but also can facilitate safe and effective immunotherapy. 15, 16 In this study recombinant Aed a 3 was proved to be an excellent candidate for use in in vivo and in vitro tests with 100% specificity in mosquito bite-negative individuals for the diagnosis of mosquito allergy.…”

Section: Discussionmentioning

confidence: 99%

Mosquito salivary allergen Aed a 3: cloning, comprehensive molecular analysis, and clinical evaluation

Peng

Sham

Lam

et al. 2016

Allergy

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Allergic reactions to mosquito bites are an increasing clinical concern. Due to lack of availability of mosquito salivary allergens, they are under-diagnosed. Here, we reported a newly cloned mosquito Aedes (Ae.) aegypti salivary allergen and its clinical relevance. A cDNA encoding a 30-kDa Ae. aegypti salivary protein, designated Aed a 3, was isolated from an expression library. The full-length cDNA was cloned into baculovirus expression vector and recombinant Aed a 3 (rAed a 3) was expressed, purified and characterized. Skin prick tests with purified rAed a 3, and Ae. aegypti mosquito bite tests were performed in 43 volunteers, some of whom had serum rAed a 3-specific IgE levels measured. The primary nucleotide sequence, deduced amino acid sequence, and IgE binding sites of Aed a 3 were identified. rAed a 3-selected antibodies recognized a 30 kDa Ae. aegypti saliva protein. rAed a 3 bound IgE in mosquito-allergic volunteers and the binding could be inhibited by addition of natural mosquito extract dose-dependently. Immediate skin test reactions to rAed a 3 correlated significantly with reactions produced by mosquito bites. Of the bite-test positive volunteers, 32% had a positive rAed a 3 skin test and 42% had specific IgE. No bite-test negative volunteers reacted to rAed a 3 in either the skin tests or the IgE assays, confirming the specificity of the assay. Aed a 3 is a major mosquito salivary allergen. Its recombinant form has biological activity and is suitable for use in skin tests and serum IgE assays in mosquito-allergic individuals.

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“…Amerikan hamamböceğine yönelik immünoterapi uygulamalarına, major allerjen ve tüm vücut ekstraktını içeren lipozom-adjuvanlı intranazal kullanılan aşılar eklenmiştir (49). Yine tüm bu zorluklardan dolayı, teşhis ve tedavide kullanılacak rekombinan allerjenler geliştirilmeye çalışılmaktadır (50 Hamamböceğine duyarlı ve evlerinde maruz kalmaya (Bla g 1 düzeyi: ≥2 U/g) devam eden bir grup hastanın Omalizumab'tan çok faydalandığı görülmüştür. Omalizumab verilenlerde, alevlenmeler %71.2 oranında azalmış, semptomlarda büyük oranda azalma ve steroid dozunda büyük düşme saptanmıştır.…”

Section: Literatürdeki Yeni Tedavi Yaklaşımlarıunclassified

Cockroach Allergy and Its Immunotherapy

Özdemir

Aydın

2016

Asthma Allergy Immunol

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ÖZHamamböceği allerjisi 30 yılı aşkın süredir, solunum yolu allerjik hastalıklarının önemli bir nedeni olarak düşünülmektedir. En sık evcil hamamböceği türleri Alman (Blattella germanica) ve Amerikan (Periplaneta americana) hamamböcekleridir. Alman hamamböceği küçük, 16 mm boyunda, tüm dünyada evlerde bulunan bir böcektir. Amerikan hamamböceği daha büyük, 35-53 mm boyutunda, ev, hastane, okul ve diğer büyük binalarda da bulunabilen bir böcektir. Türkiye' de 20' den fazla hamamböceği türü olmasına rağmen, en sık Alman hamamböceğine rastlanır. ABD' de çocuk ve erişkinleri içeren değişik araştırmalarda hamamböceği allerjisi %17-41 arasında bulunmuştur. Avrupa' da allerji kliniğine başvuran çocuk ve erişkinlerde yapılan çalışmalarda ise, %4-26 oranında saptanmıştır. Türkiye' de hamamböceği allerjisi prevalansı çocuk ve erişkinleri içeren değişik araştırmalarda %2.2-51.4 arasında bildirilmiştir. Tropomyosin, Bla g7 ve Per a7 denilen, hamamböceklerindeki major allerjenlerden biridir. Ayrıca tropomyosin, böceklerle omurgasızlar (akar, kabuklular, yumuşakca ve parazitler) arasındaki çapraz reaktivitede rol oynayan bir allerjendir. Hamamböceğine duyarlı kişilerde, diğer omurgasızların allerjenlerine kosensitizasyon yaygın bir özelliktir. Böylece, parazit enfestasyonları çapraz reaktif antikorlar aracılığıyla allerjik reaktiviteyi regüle ederler. Hamamböceği allerjen düzeyini tamamen başarılı bir temizlikten sonrası bile sürekli düşük tutmak zor olmasına ilaveten, hamamböceği allerjisinin teşhis ve tedavisinde kullanılan ticari ekstraktlar standardize değildirler. Bazı çalışmalar hamamböceğine karşı immünoterapinin etkili olduğunu düşündürmesine rağmen; kaliteli ekstraktlar, daha çok sayıda ve daha uzun süre takip edilen hastalar ile yapılacak randomize kontrollü çalışmalar bu tedavinin etkinlik ve güvenilirliğini göstermek için gereklidir. ABSTRACTCockroach allergy has been considered as an important cause of respiratory allergic diseases for over three decades. The most common domiciliary cockroach species are Blattella germanica (German cockroach) and Periplaneta americana (American cockroach). Blattella germanica is a small cockroach, approximately 16 mm in length, which commonly infests houses worldwide. Periplaneta americana is a large cockroach, approximately 35-53 mm in length, that infests houses, schools, hospitals, and other large buildings. There are more than 20 different cockroach species in Turkey, and Blattella germanica is known as the most common. In the United States, the prevalence of cockroach allergy ranges from 17 to 41%'in various studies involving both children and adults. European studies on children and adults attending an allergy clinic show a high variability in cockroach sensitisation varying from %4-26. In Turkey, cockroach allergy prevalence rate varies from %2.2-51.4 in different studies consisting of both children and adults. Tropomyosin, designated as Bla g7 and Per a7, has been identified as a major allergen in cockroaches. Tropomyosin is also an important allergen involved in cross-rea...

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Recombinant Allergens for Diagnosis of Cockroach Allergy

Cited by 35 publications

References 99 publications

Indoor Allergens and Allergic Respiratory Disease

Indoor Allergens and Allergic Respiratory Disease

Mosquito salivary allergen Aed a 3: cloning, comprehensive molecular analysis, and clinical evaluation

Cockroach Allergy and Its Immunotherapy

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