Recombinant and genetic code expanded collagen-like protein as a tailorable biomaterial

Ilamaran, Meganathan; Pachaiyappan, Mohandass; Aarthy, Mayilvahanan; Radhakrishnan, Janani; Mukherjee, Smriti; Shanmugam, Ganesh; You, Jungmok; Ayyadurai, Niraikulam

doi:10.1039/d2mh00652a

Cited by 17 publications

(12 citation statements)

References 175 publications

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“…The E. coli expression system is the most widely used protein expression system, with a low fermentation cost, short production cycle, and high efficiency. However, yeast recombinant human collagen is more similar to natural human collagen [ 28 ]. Yeast expression systems are more stable and do not produce endotoxins during purification and sterilization.…”

Section: Collagenmentioning

confidence: 99%

Application of Collagen-Based Hydrogel in Skin Wound Healing

Zhang

Wang

Liu

et al. 2023

Gels

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The repair of skin injury has always been a concern in the medical field. As a kind of biopolymer material with a special network structure and function, collagen-based hydrogel has been widely used in the field of skin injury repair. In this paper, the current research and application status of primal hydrogels in the field of skin repair in recent years are comprehensively reviewed. Starting from the structure and properties of collagen, the preparation, structural properties, and application of collagen-based hydrogels in skin injury repair are emphatically described. Meanwhile, the influences of collagen types, preparation methods, and crosslinking methods on the structural properties of hydrogels are emphatically discussed. The future and development of collagen-based hydrogels are prospected, which is expected to provide reference for the research and application of collagen-based hydrogels for skin repair in the future.

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Section: Collagenmentioning

confidence: 99%

Application of Collagen-Based Hydrogel in Skin Wound Healing

Zhang

Wang

Liu

et al. 2023

Gels

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“…[15][16][17][18][19][20] Specifically, hydrogels derived from natural polymers, such as collagen, alginate and chitosan, have been widely applied for defect-filling treatment due to their 3D aqueous network architectures, excellent biocompatibility and biodegradability. [21][22][23][24] Moreover, stimuli-responsive hydrogels, including temperature-, pH-and enzyme-responsive hydrogels, have been developed for better tissue regeneration. [25][26][27][28][29] Madry et al 30…”

Section: Introductionmentioning

confidence: 99%

“…[21][22][23][24] Moreover, stimuli-responsive hydrogels, including temperature-, pH-and enzyme-responsive hydrogels, have been developed for better tissue regeneration. [25][26][27][28][29] Madry et al 30…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, hydrogels derived from natural polymers, such as collagen, alginate and chitosan, have been widely applied for defect-filling treatment due to their 3D aqueous network architectures, excellent biocompatibility and biodegradability. 21–24 Moreover, stimuli-responsive hydrogels, including temperature-, pH- and enzyme-responsive hydrogels, have been developed for better tissue regeneration. 25–29 Madry et al 30 prepared a thermosensitive hydrogel based on poly(ethylene oxide) (PEO)–poly(propylene oxide) (PPO)–PEO poloxamers, which realized controlled release of the therapeutic recombinant adeno-associated virus (rAAV) vector and further improved full-thickness cartilage repair by overexpressing the chondrogenic Sox9 transcription factor.…”

Section: Introductionmentioning

confidence: 99%

“…21–24 Moreover, stimuli-responsive hydrogels, including temperature-, pH- and enzyme-responsive hydrogels, have been developed for better tissue regeneration. 25–29 Madry et al 30 prepared a thermosensitive hydrogel based on poly(ethylene oxide) (PEO)–poly(propylene oxide) (PPO)–PEO poloxamers, which realized controlled release of the therapeutic recombinant adeno-associated virus (rAAV) vector and further improved full-thickness cartilage repair by overexpressing the chondrogenic Sox9 transcription factor. Hu et al 31 fabricated a GelMA/nanoclay hydrogel with UV irradiation to achieve sustained release of extracellular vesicles for cartilage regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-triggered in situ gelation with ROS-controlled drug release for cartilage repair

Wu,

Zhang,

Wang

et al. 2023

Mater. Horiz.

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Thiolation‐Based Protein–Protein Hydrogels for Improved Wound Healing

Liu,

Guo,

Wang

et al. 2024

Adv Healthcare Materials

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The limitations of protein‐based hydrogels, including their insufficient mechanical properties and restricted biological functions, arise from the highly specific functions of proteins as natural building blocks. A potential solution to overcome these shortcomings is the development of protein‐protein hydrogels, which integrate structural and functional proteins. In this study, we introduce a protein‐protein hydrogel formed by crosslinking bovine serum albumin (BSA) and a genetically engineered intrinsically disordered collagen‐like protein (CLP) through Ag‐S bonding. Our approach involves thiolating lysine residues of BSA and crosslinking CLP with Ag+ ions, utilizing thiolation of BSA and the free‐cysteines of CLP. The resulting protein‐protein hydrogels exhibit exceptional properties, including notable plasticity, inherent self‐healing capabilities, and gel‐sol transition in response to redox conditions. In comparison to standalone BSA hydrogels, these protein‐protein hydrogels demonstrate enhanced cellular viability, and improved cellular migration. In vivo experiments provide conclusive evidence of accelerated wound healing, observed not only in murine models with streptozotocin (Step)‐induced diabetes but also in zebrafish models subjected to UV‐burn injuries. Detailed mechanistic insights, combined with assessments of pro‐inflammatory cytokines and the expression of epidermal differentiation‐related proteins, robustly validate the protein‐protein hydrogel's effectiveness in promoting wound repair.This article is protected by copyright. All rights reserved

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Recombinant and genetic code expanded collagen-like protein as a tailorable biomaterial

Abstract: Collagen occurs in nature with a dedicated triple helix structure and is the most preferred biomaterial in commercialized medical products. Recombinant collagen emerge as sustainable alternate source that overcomes existing demerits.

Cited by 17 publications

References 175 publications

Application of Collagen-Based Hydrogel in Skin Wound Healing

Application of Collagen-Based Hydrogel in Skin Wound Healing

Ultrasound-triggered in situ gelation with ROS-controlled drug release for cartilage repair

Thiolation‐Based Protein–Protein Hydrogels for Improved Wound Healing

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