RECOMBINANT ANTIBODY PRODUCTION BY CLONING OF Pepper yellow leaf curl Indonesia virus (PepYLCIV) COAT PROTEIN GENE

Paradisa, Yashanti Berlinda; Sulandari, Sri; Hartono, Slamet; Somowiyarjo, Susamto; Windarningsih, Mery; Sari, Dini Wahyu Kartika; Handayani, Christina Retna

doi:10.23960/jhptt.1221-13

Cited by 3 publications

(3 citation statements)

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“…The observed PepYLCIV group is similar to MN738463 PepYLCIV Chili pepper Indonesia, MN094866 PepYLCIV Capsicum annum Indonesia, AB267838 PepYLCIV Ageratum Indonesia, DQ083765 PepYLCIV Thai Pepper, and KX900491 PepYLCIV Thai Pepper. Paradisa et al (2022) explained that the PepYLCIV group also has over 93% genetic similarity (homology) with PepYLCIV in Congkrang, Muntilan, Central Java, in North Sumatra-Chile LC051112, in East Java Chile MN738463, in Bali-Pseuderanthemum MN094868, and Bogor-Chile DQ083764. It was further explained that Thai Pepper DQ083765 PepYLCIV has 100% genetic similarity with PepYLCIV AB267834, PepYLCIV NC008283, and PepYLCIV DQ083764 (Sinha et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

WIDODO

TAUFIK²,

KHAERUNI³

et al. 2023

Biodiversitas

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Abstract. Widodo CJ, Taufik M, Khaeruni A, Mallaranggeng R. 2023. Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia. Biodiversitas 24: 741-751. Begomovirus disease is one of the main factors inhibiting chili cultivation which could harm plants and result in crop failure. This study aimed to determine the symptoms of begomovirus disease in chili plants in Buton and Muna, Southeast Sulawesi. The methods used are disease incidence survey, insect vector observation, molecular identification using the polymerase chain reaction (PCR) technique, and Sanger sequencing (Applied Biosystem 3500). The survey was conducted in Southeast Sulawesi province, covering Bau-Bau City, Buton Regency, Central Buton, North Buton, Muna, and West Muna. The results showed that the incidence and severity of Begomovirus disease were observed highest in Buton islands, 73% and 49.5% (Buton City), followed by North Buton, Central Buton, and Buton, with the incidence and severity of Begomovirus, respectively, of 71.74% and 59.5%; 69.32% and 51.5%; and 68% and 61.5%. While in Muna Regency, 63.16% and 38.5%, West Muna, 73.79% and 53.50%. On the other hand, the highest percentage of the whitefly population was in Bau-Bau City, 24%, while the lowest was in West Muna, 15.5% supporting this observation. Furthermore, PCR analysis successfully amplified symptomatic isolates from Buton and Muna islands at a band length of 580 bp. On the other hand, Sanger sequencing analysis revealed the existence of the Indonesian Pepper Yellow Leaf Curl Virus (PepYLCIV) with more than 96% genetic homology to the Gene Bank data of MN094866 PepYLCIV Capsicum annuum Indonesia and MN738463 PepYLCIV Chili pepper Indonesian. This information is the first report of the presence of Begomovirus on the two islands.

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Section: Discussionmentioning

confidence: 99%

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

WIDODO

TAUFIK²,

KHAERUNI³

et al. 2023

Biodiversitas

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“…Nucleic acid-based molecular detection methods are frequently employed in laboratories because of their precision and sensitivity [3]. PCR is the most widely used method for detecting PepYLCV [1,[5][6][7]. However, Malathi [8] states that detecting and characterizing begomoviruses is difficult due to very low virus concentrations and difficulties to obtaining high-quality DNA suitable for PCR from plants grown in the field, particularly plants with high tannin and mucilage concentrations.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of three different DNA extraction methods for the detection of Pepper yellow leaf curl virus (PepYLCV) by Polymerase Chain Reaction

Paradisa,

Hidayat,

Saputra

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Pepper yellow leaf curl virus (PepYLCV) from the genus Begomovirus infecting Chili pepper plant can cause significant yield losses. PepYLCV infection can lead the development of mosaic yellow, mottled pattern and yellowing leaves, which can be challenging in disease management and control. Developing effective detection methods for PepYLCV is a critical disease management and crop loss mitigation. The Begomovirus viruses have extremely low viral concentrations and are limited to the phloem and vascular system. DNA extraction is an important step in PepYLCV detection. This study aimed to evaluate the efficiency of three DNA extraction methods: phenol-based extraction, CTAB-based extraction, and the GeneJET Plant Genomic DNA Purification Kit (Thermo Scientific™). These methods were evaluated for their performance to identified the presence of PepYLCV DNA and provide accurate results for further analyses. DNA samples were extracted from two varieties including Red Habanero and F8 012328-6-2-1-1-3-1, and were subsequently amplified using Krusty Homer’s primers. The results showed that all three extraction methods possess the capability to identify PepYLCV. Based on the results, DNA extraction employing the CTAB-based method yields DNA with higher concentration and purity levels. Additionally, this method proves to be cost-effective and proficient in generating higher DNA concentrations.

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“…In Indonesia, several Begomovirus species have also been reported, including ToLCJaV, AYVV, TYLCKaV, PepYLCIV, Mungbean yellow mosaic India virus (MYMIV) Tomato leaf curl New Delhi virus (ToLCNDV), and Squash leaf curl China virus (SLCCV) (Kon et al, 2007;Nurulita et al, 2015;Paradisa et al, 2022;Selangga & Listihani, 2021;Subiastuti et al, 2019;Sutrawati et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Begomoviruses on two chili types in Southeast Sulawesi Indonesia: variation of symptom severity assessment and DNA-betasatellite identification

Taufik,

Firihu,

Hasan

et al. 2023

J Trop Plant Pests Dis

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The association of viral satellite DNA with Begomoviruses influences symptom expression in infected plants. Normalized Difference Vegetation Index (NDVI) is an image processing method used to assess plant health based on the plant’s ability to absorb sunlight for photosynthesis. Therefore, this study aims to assess symptom severity based on symptom variation and NDVI, as well as to detect and identify the presence of beta-satellite DNA associated with chili plants. The study was conducted in North Kolaka Regency, Southeast Sulawesi Province, Indonesia. It involved observations and image recording of symptom variations in the sampled cayenne and big red chili plants, followed by the detection of beta-satellite DNA in the samples using the polymerase chain reaction (PCR). The results confirmed the presence of non-coding satellite DNA associated with the Pepper yellow leaf curl Indonesia virus (PepYLCIV) in all severe, mild, or asymptomatic plant samples. In the phylogenetic tree, the non-coding satellite DNA isolates from Southeast Sulawesi are included in the betasatellite group and exhibit different genetic characteristics from other isolates available in the GenBank database. The cayenne chili with severe symptoms had lower NDVI values than the mild and asymptomatic categories, indicating that this type of chili with severe symptoms had a lower ability to absorb light. Further studies are needed to characterize the alpha-satellite type in the Southeast Sulawesi region for potential use in cross-protection mechanisms against plant virus infections.

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RECOMBINANT ANTIBODY PRODUCTION BY CLONING OF Pepper yellow leaf curl Indonesia virus (PepYLCIV) COAT PROTEIN GENE

Cited by 3 publications

References 0 publications

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

Evaluation of three different DNA extraction methods for the detection of Pepper yellow leaf curl virus (PepYLCV) by Polymerase Chain Reaction

Begomoviruses on two chili types in Southeast Sulawesi Indonesia: variation of symptom severity assessment and DNA-betasatellite identification

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