Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals

Denison, Michael S.; Zhao, Bin; Baston, David S.; Clark, George C.; Murata, Hiroshi; Han, Dalho

doi:10.1016/j.talanta.2004.05.032

Cited by 91 publications

(98 citation statements)

References 95 publications

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“…Instrumental analysis does not account for interactions among the chemicals in complex mixtures and provides little information on their biological effects (Brack 2003;Rijk et al 2009). Therefore, the instrumental results are not well suited to predict the toxicity of unknown chemicals in the system, nor the complex interactions of known chemicals that could lead to additivity, antagonism, and synergism on biota (Villeneuve et al 2000;Denison et al 2004). Furthermore, chemicals that are biologically active at concentrations below the analytical detection limits are missed by instrumental analysis.…”

Section: Introductionmentioning

confidence: 99%

Aryl hydrocarbon receptor (AhR) inducers and estrogen receptor (ER) activities in surface sediments of Three Gorges Reservoir, China evaluated with in vitro cell bioassays

Wang

Bovee²,

et al. 2013

Environ Sci Pollut Res

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Two types of biological tests were employed for monitoring the toxicological profile of sediment cores in the Three Gorges Reservoir (TGR), China. In the present study, sediments collected in June 2010 from TGR were analyzed for estrogen receptor (ER)-and aryl hydrocarbon receptor (AhR)-mediated activities. The estrogenic activity was assessed using a rapid yeast estrogen bioassay, based on the expression of a green fluorescent reporter protein. Weak anti-estrogenic activity was detected in sediments from an area close to the dam of the reservoir, and weak estrogenic activities ranging from 0.3 to 1 ng 17β-estradiol (E2) equivalents (EQ) g −1 dry weight sediment (dw) were detected in sediments from the Wanzhou to Guojiaba areas. In the upstream areas Wanzhou and Wushan, sediments demonstrated additive effects in coadministration of 1 nM E2 in the yeast test system, while sediments from the downstream Badong and Guojiaba areas showed estrogenic activities which seemed to be more than additive (synergistic activity). There was an increasing tendency in estrogenic activity from upstream of TGR to downstream, while this tendency terminated and converted into anti-estrogenic activity in the area close to the dam. The AhR activity was detected employing rat hepatoma cell line (H4IIE). EROD activities were found homogenously distributed in sediments in TGR ranging from 200 to 311 pg 2,3,7,8-tetrachlorodibenzo-p -dioxin (TCDD) EQ g −1 dw for total AhR agonists and from 45 to 76 pg TCDD EQ g −1 dw for more persistent AhR agonists. The known AhR agonists polycyclic aromatic hydrocarbon, polychlorinated biphenyl, and PCDD/F only explained up to 8 % of the more persistent AhR agonist activity in the samples, which suggests that unidentified AhR-active compounds represented a great proportion of the TCDD EQ in sediments from TGR. These findings of estrogenic potential and dioxin-like activity in TGR sediments provide possible weight-of-evidence of potential ecotoxicological causes for the declines in fish populations which have been observed during the past decades in TGR.

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Section: Introductionmentioning

confidence: 99%

Aryl hydrocarbon receptor (AhR) inducers and estrogen receptor (ER) activities in surface sediments of Three Gorges Reservoir, China evaluated with in vitro cell bioassays

Wang

Bovee²,

et al. 2013

Environ Sci Pollut Res

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“…More recently, the use of in vitro bioassays based on AHR-regulated genes has increased for estimating HAH concentrations in environmental media, for example, US EPA Method 4425 (Hu et al, 1995;Giesy et al, 1997;Pauwels et al, 2000;US EPA, 2000;Koppen et al, 2001;Covaci et al, 2002;Denison et al, 2004). A commonly used commercially available cell bioassay is the chemical-activated luciferase gene expression (CALUX) bioassay that measures the ability of a chemical mixture to activate AHR-dependent gene expression of the firefly luciferase gene in genetically modified cell lines relative to activation by TCDD (Aarts et al, 1995;Garrison et al, 1996;Murk et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…A commonly used commercially available cell bioassay is the chemical-activated luciferase gene expression (CALUX) bioassay that measures the ability of a chemical mixture to activate AHR-dependent gene expression of the firefly luciferase gene in genetically modified cell lines relative to activation by TCDD (Aarts et al, 1995;Garrison et al, 1996;Murk et al, 1996). The CALUX bioassay typically utilizes an immortalized hepatomaderived cell line either from rats or mice (Murk et al, 1996;Denison et al, 2004). The results from the CALUX bioassay are often reported in TCDD equivalencies (TEQs) and compared against analytical TEQs determined for the mixture using GC-MS mass data and HAH congener-specific toxicity equivalency factors (TEFs; van den Berg et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

AH receptor agonist activity in human blood measured with a cell-based bioassay: Evidence for naturally occurring AH receptor ligands in vivo

Connor¹,

Harris²,

Edwards

et al. 2007

J Expo Sci Environ Epidemiol

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In the present study, an aryl hydrocarbon receptor (AHR)-driven reporter gene bioassay was used to measure the activity, measured as an induction equivalent (IEQ) as compared to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or IEQ concentration in human blood samples from 10 volunteers under different dietary regimens. Blood concentrations of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and polychlorinated biphenyls (PCBs), as determined by analytical chemistry (HR-GC/MS), and expressed as toxic equivalents (TEQs) with the use of TCDD equivalency factors (TEFs), were within a range that has been reported in the general US population, ranging from 0.022 to 0.119 ppt (whole blood basis). However, the human blood IEQ measured directly via bioassay ranged from 13.4 to 218 ppt (whole blood basis). These order of magnitude greater IEQs compared to the TEQs for dioxins, furans, and certain PCBs suggests that human blood contains a relatively high level of AHR agonists able to activate the CYP1A1 dioxin response element (DRE)-linked reporter gene bioassay and that this AHR activity is not accounted for by PCDDs/Fs and dioxin-like PCBs based on standard HR-GC/MS and TEF analysis. When study participants switched from a ''baseline'' to a high-vegetable diet, increases in bioassay IEQ were observed that were statistically significant (Po0.05). In addition, IEQ activity was elevated above levels observed following dietary intervention in two subjects given indole-3-carbinol (I3C) supplements. We conclude that a substantial portion of the IEQ activity occurred as a result of the increased intake of natural AHR agonists (NAHRAs) present in many fruits, vegetables. and herbs. Our findings also suggest that dietary NAHRAs constitute a substantial daily dietary intake of AHR-active compounds, and these NAHRAs could influence AHR status in humans and play a role in a basal level of AHR activation.

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“…The CALUX method has been explained by various authors (Denison et al, 2004(Denison et al, , 2002Murk, 1996;). It uses genetically modified cells (hepatoma cells stably transfected with a luciferase reporter gene) which respond to chemicals that activate the cytosolar AhR by induction of luciferase (Denison et al, 2004).…”

mentioning

confidence: 99%

“…It uses genetically modified cells (hepatoma cells stably transfected with a luciferase reporter gene) which respond to chemicals that activate the cytosolar AhR by induction of luciferase (Denison et al, 2004). By this method, the toxicity of pollutants such as PCDD/Fs and dioxin like-PCBs is produced either as a change in gene expression mediated through the AhR or by interference with other pathways (Hurst, Balaam, Chan-Man, Thain, & Thomas, 2004).…”

mentioning

confidence: 99%

PCDD/F and Dioxin-Like PCB Determinations in Mtoni Estuarine Sediments (Tanzania) Using the Chemically Activated Luciferase Gene Expression (CALUX) Bioassay

Mihale

Croes

Tungaraza

et al. 2013

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Sediments from Mtoni estuary and 2 tributaries, Tanzania, were screened for polychlorinated-p-dibenzodioxins, -dibenzofurans (PCDD/Fs) and dioxin-like PCBs (dl-PCBs) using the chemically activated luciferase gene expression (CALUX) bioassay approach. PCDD/Fs expressed as bio analytical equivalence (BEQ) values ranged from 5.7 ± 1.4 to 39.9 ± 5.8 pg BEQ/g sediment in the wet season and from 14.1 ± 2.0 to 32.8 ± 4.7 pg BEQ/g sediment in the dry season, with higher levels observed in Kizinga River and stations close to the mouth of that river. Dioxin-like PCB levels ranged from 0.21 ± 0.03 to 0.53 ± 0.03 pg BEQ/g sediment in the wet season and from 0.22 ± 0.03 to 0.59 ± 0.04 pg BEQ/g sediment in the dry season. Higher PCDD/F and dl-PCB levels in sediments are probably related to open burning of plastic scraps, household burning of wood or charcoal and traffic related emissions, all of which occur in the Dar es Salaam region. The denser population and the more intense industrial activities in the Kizinga River basin may explain the enhanced PCDD/F and dl-PCB levels observed in the sediments of that river compared to the levels in the Mzinga River basin. A third sampling campaign, including also stations in the downstream estuary, confirmed the enhanced levels in the Kizinga River (maximum of 400 pg-BEQ/g) and also showed that a clear decreasing concentration gradient in the downstream direction exists. It cannot be excluded that the levels of these pollutants in the sediments of the Mtoni estuary pose a threat to the local biological community.

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Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals

Cited by 91 publications

References 95 publications

Aryl hydrocarbon receptor (AhR) inducers and estrogen receptor (ER) activities in surface sediments of Three Gorges Reservoir, China evaluated with in vitro cell bioassays

Aryl hydrocarbon receptor (AhR) inducers and estrogen receptor (ER) activities in surface sediments of Three Gorges Reservoir, China evaluated with in vitro cell bioassays

AH receptor agonist activity in human blood measured with a cell-based bioassay: Evidence for naturally occurring AH receptor ligands in vivo

PCDD/F and Dioxin-Like PCB Determinations in Mtoni Estuarine Sediments (Tanzania) Using the Chemically Activated Luciferase Gene Expression (CALUX) Bioassay

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