2021
DOI: 10.1186/s12934-021-01642-y
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Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification

Abstract: Background The use of bovine-origin ribonucleases has been part of the standard protocol for plasmid DNA purification. As the field of gene therapy now enters the clinical stage, such enzymes need to be phased out or alternative purification protocols need to be developed to ensure product safety and regulatory compliance. The recombinant expression of bacterial RNase is fraught with toxicity problems making it a challenging enzyme to express. The current study describes a plasmid construct tha… Show more

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Cited by 4 publications
(1 citation statement)
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“…1186/ s12934-021-01642-y. *Correspondence: ram.shankar@plasmidfactory.com; martin.schleef@uni-bielefeld.de 1 PlasmidFactory GmbH & Co. KG, Meisenstrasse 96, 33607 Bielefeld, Germany Full list of author information is available at the end of the article…”
Section: Publisher's Notementioning
confidence: 99%
“…1186/ s12934-021-01642-y. *Correspondence: ram.shankar@plasmidfactory.com; martin.schleef@uni-bielefeld.de 1 PlasmidFactory GmbH & Co. KG, Meisenstrasse 96, 33607 Bielefeld, Germany Full list of author information is available at the end of the article…”
Section: Publisher's Notementioning
confidence: 99%