Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection

Yaacob, Eamy Nursaliza; Geest, Bruno G. De; Goethals, Joke; Bajek, Anna; Dierckens, Kristof; Bossier, Peter; Vanrompay, Daisy

doi:10.1016/j.vetimm.2018.09.001

Cited by 6 publications

(5 citation statements)

References 59 publications

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“…Actually, only few other immune-related genes were upregulated following infection. The latter is in contradiction with our previous results using the same experimental infection model, the same V. anguillarum strain and the same infective dose (43). During the latter study, V. anguillarum highly upregulated the expression of all genes under study (the same gene panel as currently with the exception of hpc and dic) except for cxcr4 and ccr9.…”

Section: Figure 5 |contrasting

confidence: 99%

“…The expression of sensor (tlr3, tlr5m, cxcr4, ccr9), regulatory (il10, cas1, il1β, tnfα, mif1, cxcl8) and effector (hpc, dic) genes was studied early upon infection as the first time points of infection are critical for fish immunity (67). Previously, we demonstrated the expression of all sensor and regulatory genes under study in axenic sea bass larvae (43). The expression of hpc and dic has been demonstrated by others in axenic and non-axenic sea bass larvae, respectively (44,67).…”

Section: Figure 5 |mentioning

confidence: 99%

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Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

et al. 2020

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Vibrio anguillarum causes high mortality in European sea bass (Dicentrarchus labrax) larviculture and is a hindering factor for successful sustainable aquaculture of this commercially valuable species. Priming of the innate immune system through administration of immunostimulants has become an important approach to control disease outbreaks in marine fish larviculture. This study was conducted to evaluate immunostimulation by Escherichia coli HSP70 (DnaK) in axenic European sea bass larvae in order to protect the larvae against vibriosis. DnaK stimulates the immune response in crustaceans and juvenile fish against bacterial infections. The use of axenic fish larvae allows to study immunostimulation in the absence of an interfering microbial community. At 7 days post-hatching, larvae received a single dose of alginate encapsulated recombinant DnaK. Two non-treated control groups in which animals either received empty alginate microparticles (C1) or no alginante microparticles (C2 and C3) were included in the study. Eighteen hours later, all larvae, except the ones from group C3 (non-infected control) were challenged with V. anguillarum (10 5 CFU, bath infection). Mortality was daily recorded until 120 h post infection and at 18, 24, and 36 h post infection, larvae were sampled for expression of immune related genes. Results showed that V. anguillarum induced an immune response in axenic sea bass larvae but that the innate immune response was incapable to protect the larvae against deadly septicaemic disease. In addition, we showed that administration of alginate encapsulated DnaK to axenic European sea bass larvae at DAH7 resulted in a significant, DnaK dose dependent, upreglation of immune sensor, regulatory and effector genes. Significant upregulation of cxcr4, cas1 and especially of hep and dic was correlated with significant higher survival rates in V. anguillarum infected larvae. In the future recombinant DnaK might perhaps be used as a novel immunostimulant in sea bass larviculture.

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Section: Figure 5 |contrasting

confidence: 99%

Section: Figure 5 |mentioning

confidence: 99%

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

et al. 2020

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“…In the first one, after 400 ATUs, Atlantic salmon were vaccinated with IPath ® against the bacterium A. salmonicida. Unlike reported in sea bass larvae exposed to recombinant ferritin and subsequently infected with Vibrio anguillarum [22], IPath ® -vaccinated fish groups showed high activity of MCHII and IL-1β genes in response to A. salmonicida. Among the immune genes associated with fish response to C. rogercresseyi, MHCII and TLR22 genes have been associated with inflammatory responses [62].…”

Section: Discussioncontrasting

confidence: 82%

“…Moreover, the antiviral activity of two isoforms of hepcidin has been reported in turbot [21]. On the other hand, immunosuppression effects have been reported in European sea bass larvae exposed to recombinant ferritin during Vibrio anguillarum infection [22]. Moreover, for terrestrial organisms, recombinant ferritin has been used for ectoparasite control [23].…”

Section: Introductionmentioning

confidence: 99%

Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases

et al. 2021

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Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins’ expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish’s immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath®) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath® protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath® was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath® and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium Aeromonas salmonicida, and in a second trial, fish were exposed to the ectoparasite Caligus rogercresseyi and subsequently infected with the intracellular bacterium Piscirickettsia salmonis. Fish immunized with IPath® showed a significant delay in the mortality curve in response to A. salmonicida and P. salmonis infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high modulation of iron-homeostasis-related genes in fish vaccinated with IPath® compared to the control group during the infection. Moreover, increasing expression of Atlantic salmon IgT was associated with IPath® immunization. This study provides evidence that the IPath® protein could be used as an antigen or booster in commercial fish vaccines, improving the immune response against relevant pathogens for salmon aquaculture.

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“…Negative roles of ferritins in anti-disease were also observed in other fish species. For instance, in sea bass, recombinant ferritin-H function in the immune-suppression reaction of CXCR4, which is detrimental to innate immunity [ 18 ]. In addition to fishes, the ability of ferritin to enhance immune function is widely reported in other species [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Sequence, Expression, and Anti-GCRV Function of the Ferritin from the Grass Carp, Ctenopharyngodon idellus

Xiao

Tang

et al. 2022

IJMS

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Ferritin possesses an immune function to defend against pathogen infection. To elucidate the immunity-protecting roles of ferritin from Ctenopharyngodon idellus (Ciferritin) against virus infection, the cDNA and promoter sequences of Ciferritin were determined, and the correlations between Ciferrtin expressions and promoter methylation levels were analyzed. In addition, the functional role of Ciferrtin on GCRV (grass carp reovirus) infection was assessed. The full-length cDNA of Ciferritin is 1053 bp, consists of a 531 bp open-reading frame, and encodes 176 amino acids. Ciferritin showed the highest sequence identity with the ferritin middle subunit of Mylopharyngodon piceus (93.56%), followed by the subunits of Megalobrama amblycephala and Sinocyclocheilus rhinocerous. Ciferritin contains a conserved ferritin domain (interval: 10–94 aa), and the caspase recruitment domain (CARD) and Rubrerythrin domain were also predicted. In the spleen and kidney, significantly higher Ciferritin expressions were observed at 6, 12, 24, or 168 h post GCRV infection than those in the PBS injection group (p < 0.05). The Ciferrtin expression level in the progeny of maternal-immunized grass carp was significantly higher than that in the progeny of common grass carp (p < 0.05). Ciferritin promoter methylation level in the progeny from common grass carp was 1.27 ± 0.15, and in the progeny of the maternal-immunized group was 1.00 ± 0.14. In addition, methylation levels of “CpG9” and “CpG10” loci were significantly lower in the progeny of maternal-immunized fish than those in the common group. Except for the “CpG5”, methylation levels of all other detected “CpG” loci negatively correlated with Ciferritin expression levels. Furthermore, the total methylation level of “CpG1–10” negatively correlated with the Ciferritin expressions. The Ciferritin expression level was significantly up-regulated, and the VP7 protein levels were significantly reduced, at 24 h post GCRV infection in the Ciferritin over-expression cells (p < 0.05). The results from the present study provide sequence, epigenetic modification and expression, and anti-GCRV functional information of Ciferritin, which provide a basis for achieving resistance to GCRV in grass carp breeding.

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Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection

Cited by 6 publications

References 59 publications

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases

Sequence, Expression, and Anti-GCRV Function of the Ferritin from the Grass Carp, Ctenopharyngodon idellus

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