Recombinant fibromodulin and decorin effects on NF-κB and TGFβ1 in the 4T1 breast cancer cell line

Nejad, Ladan Dawoody; Biglari, Alireza; Annese, Tiziana; Ribatti, Doménico

doi:10.3892/ol.2017.5960

Cited by 21 publications

(18 citation statements)

References 51 publications

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“…We previously reported that serum decorin levels are positively correlated with skeletal muscle mass in patients with HCC [16]. Decorin is also reported to interact with transforming growth factor-β and receptors of tyrosine kinase such as epidermal and insulin-like growth factors [17], leading to suppression of proliferation of various tumor cell lines, including HCC cell lines [18][19][20]. In addition, decorin is known to be expressed in various tissues including intestinal tissue, cardiac tissue, and adipose tissue and is known to regulate autophagy, inflammation, and glucose homeostasis [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Impact of Decorin on the Physical Function and Prognosis of Patients with Hepatocellular Carcinoma

Kawaguchi

Yoshio

Sakamoto

et al. 2020

JCM

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The outcome of patients with hepatocellular carcinoma (HCC) is still poor. Decorin is a small leucine-rich proteoglycan, which exerts antiproliferative and antiangiogenic properties in vitro. We aimed to investigate the associations of decorin with physical function and prognosis in patients with HCC. We enrolled 65 patients with HCC treated with transcatheter arterial chemoembolization (median age, 75 years; female/male, 25/40). Serum decorin levels were measured using enzyme-linked immunosorbent assays; patients were classified into the High or Low decorin groups by median levels. Associations of decorin with physical function and prognosis were evaluated by multivariate correlation and Cox regression analyses, respectively. Age and skeletal muscle indices were not significantly different between the High and Low decorin groups. In the High decorin group, the 6-min walking distance was significantly longer than the Low decorin group and was significantly correlated with serum decorin levels (r = 0.2927, p = 0.0353). In multivariate analysis, the High decorin group was independently associated with overall survival (hazard ratio 2.808, 95% confidence interval 1.016–8.018, p = 0.0498). In the High decorin group, overall survival rate was significantly higher than in the Low decorin group (median 732 days vs. 463 days, p = 0.010). In conclusion, decorin may be associated with physical function and prognosis in patients with HCC.

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Section: Introductionmentioning

confidence: 99%

Impact of Decorin on the Physical Function and Prognosis of Patients with Hepatocellular Carcinoma

Kawaguchi

Yoshio

Sakamoto

et al. 2020

JCM

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“…It has been proven that TGF-β is involved in cell development, angiogenesis, immune response, and inflammation [28][29][30]; signaling disorders involving TGF-β lead to multiple pathological processes, including cancer [31]. Studies have shown that in the case of Fmod−/−, deficient migration can be relieved by adding exogenous TGF-β1, which indicates that the absence of Fmod alters the normal temporospatial pattern [32]. Zheng et al showed that Fmod interacts with TGF-β during cutaneous repair and scar formation; wound closure can be delayed in Fmod-deficient mice, which is accompanied by an increase of TGF-β3 signaling [7].…”

Section: Discussionmentioning

confidence: 99%

Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway

Yin

Cui

Han

et al. 2020

Animals

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Fibromodulin (Fmod), which is an extracellular matrix protein, belongs to the extracellular matrix small-leucine-rich proteoglycan family. Fmod is abundantly expressed in muscles and connective tissues and is involved in biological regulation processes, including cell apoptosis, cell adhesion, and modulation of cytokine activity. Fmod is the main regulator of myostatin, which controls the development of muscle cells, but its regulatory path is unknown. Chicken models are ideal for studying embryonic skeletal muscle development; therefore, to investigate the mechanism of Fmod in muscle development, Fmod-silenced and Fmod-overexpressed chicken myoblasts were constructed. The results showed that Fmod plays a positive role in differentiation by detecting the expression of myogenic differentiation markers, immunofluorescence of MyHC protein, and myotube formation in myoblasts. Fmod regulates expression of atrophy-related genes to alleviate muscle atrophy, which was confirmed by histological analysis of breast muscles in Fmod-modulated chicks in vivo. Additionally, genes differentially expressed between Fmod knockdown and normal myoblasts were enriched in the signaling pathway of transforming growth factor β (TGF-β). Both Fmod-silenced and Fmod-overexpressed myoblasts regulated the expression of TGFBR1 and p-Smad3. Thus, Fmod can promote differentiation but not proliferation of myoblasts by regulating the TGF-β signaling pathway, which may serve a function in muscular atrophy.

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“…Fmod has been shown to successfully inhibit the nuclear factor-kB (NF-kB) signaling and induce fibroblast apoptosis (Lee and Schiemann, 2011). Dawoody Nejad et al, 2017 demonstrated that recombinant Fmod was able to suppress TGFb1 and NF-kB activity in vitro in a highly metastatic breast cancer cell line (Dawoody Nejad et al, 2017).…”

Section: Fibromodulinmentioning

confidence: 99%

Proteoglycans in Biomedicine: Resurgence of an Underexploited Class of ECM Molecules

Walimbe

Panitch

2020

Front. Pharmacol.

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Proteoglycans have emerged as biomacromolecules with important roles in matrix remodeling, homeostasis, and signaling in the past two decades. Due to their negatively charged glycosaminoglycan chains as well as distinct core protein structures, they interact with a variety of molecules, including matrix proteins, growth factors, cytokines and chemokines, pathogens, and enzymes. This led to the dawn of glycan therapies in the 20 th century, but this research was quickly overshadowed by readily available DNA and protein-based therapies. The recent development of recombinant technology and advances in our understanding of proteoglycan function have led to a resurgence of these molecules as potential therapeutics. This review focuses on the recent preclinical efforts that are bringing proteoglycan research and therapies back to the forefront. Examples of studies using proteoglycan cores and mimetics have also been included to give the readers a perspective on the wide-ranging and extensive applications of these versatile molecules. Collectively, these advances are opening new avenues for targeting diseases at a molecular level, and providing avenues for the development of new and exciting treatments in regenerative medicine.

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Recombinant fibromodulin and decorin effects on NF-κB and TGFβ1 in the 4T1 breast cancer cell line

Cited by 21 publications

References 51 publications

Impact of Decorin on the Physical Function and Prognosis of Patients with Hepatocellular Carcinoma

Impact of Decorin on the Physical Function and Prognosis of Patients with Hepatocellular Carcinoma

Fibromodulin Modulates Chicken Skeletal Muscle Development via the Transforming Growth Factor-β Signaling Pathway

Proteoglycans in Biomedicine: Resurgence of an Underexploited Class of ECM Molecules

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