Recombinant Fibronectin Fragment III8-10/polylactic Acid Hybrid Nanofibers Enhance the Bioactivity of Titanium Surface

Guillem-Marti, Jordi; Boix‐Lemonche, Gerard; Gugutkov, Dencho; Ginebra, Maria‐Pau; Altankov, George; Manero, José María

doi:10.2217/nnm-2017-0342

Cited by 5 publications

(7 citation statements)

References 57 publications

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“…Noteworthy, the mineralization at 21 days was also higher when the Ti scaffolds were functionalized with CAS compared to the nonfunctionalized scaffolds (Figure 8c). These results are in agreement with previous studies, notwithstanding that it was found that the CAS fragments did not stimulate cell differentiation nor mineralization per se [35,60], when the CAS fragments were present in a three-dimensional matrix synergistic effects on cell differentiation were found between the CAS fragments, the increased roughness and the three-dimensional architecture [46]. Similarly, in this case, we hypothesize that the three-dimensional architecture of the scaffolds along with the filament roughness and the presence of CAS synergistically enhanced the differentiation and mineralization of SaOS-2 cells [54,61].…”

Section: In Vitro Cell Responsesupporting

confidence: 93%

“…The CAS fragment has been described to promote cell proliferation in smooth Ti surfaces [31]. Moreover, Guillem-Marti et al have studied the adhesion and proliferation of SaOS-2 cells on smooth Ti, obtaining similar results on adhesion and proliferation than the Ti scaffolds used as a control in this work, which demonstrates the biocompatibility of uncoated Ti [46,57]. In this study, however, the Ti surface presents pores that have been shown to affect protein adsorption processes through capillary forces and increased specific surface area [58].…”

Section: In Vitro Cell Responsesupporting

confidence: 64%

“…However, a significant reduction in scaffold pore size was observed when the sintering temperature raised from 1200 • C to 1300 • C (Table 2), due to the sample shrinkage during sintering. In all cases, the scaffold pore size was within the acceptable range to allow bone ingrowth, vascularization and transport of metabolic products, according to the literature [8,9,[44][45][46].…”

Section: Scaffolds Characterizationmentioning

confidence: 85%

“…Moreover, the cells attached to CAS-functionalized scaffolds were more spread than those attached to pristine Ti scaffolds, where the cells were less flattened and some of them even presented a rounded shape morphology (Figure 7). The improved adhesion and spreading should be attributed to the adhesive capacities of the CAS fragment, which contains the RGD sequence along with the synergistic PHSRN sequence, both mediating the integrin interaction and the creation of focal adhesions [46,56]. (Figure 8b).…”

Section: In Vitro Cell Responsementioning

confidence: 99%

See 3 more Smart Citations

Titanium Scaffolds by Direct Ink Writing: Fabrication and Functionalization to Guide Osteoblast Behavior

Vidal

Torres

Guillem-Marti

et al. 2020

Metals

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Titanium (Ti) and Ti alloys have been used for decades for bone prostheses due to its mechanical reliability and good biocompatibility. However, the high stiffness of Ti implants and the lack of bioactivity are pending issues that should be improved to minimize implant failure. The stress shielding effect, a result of the stiffness mismatch between titanium and bone, can be reduced by introducing a tailored structural porosity in the implant. In this work, porous titanium structures were produced by direct ink writing (DIW), using a new Ti ink formulation containing a thermosensitive hydrogel. A thermal treatment was optimized to ensure the complete elimination of the binder before the sintering process, in order to avoid contamination of the titanium structures. The samples were sintered in argon atmosphere at 1200 °C, 1300 °C or 1400 °C, resulting in total porosities ranging between 72.3% and 77.7%. A correlation was found between the total porosity and the elastic modulus of the scaffolds. The stiffness and yield strength were similar to those of cancellous bone. The functionalization of the scaffold surface with a cell adhesion fibronectin recombinant fragment resulted in enhanced adhesion and spreading of osteoblastic-like cells, together with increased alkaline phosphatase expression and mineralization.

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Section: In Vitro Cell Responsesupporting

confidence: 93%

Section: In Vitro Cell Responsesupporting

confidence: 64%

Section: Scaffolds Characterizationmentioning

confidence: 85%

Section: In Vitro Cell Responsementioning

confidence: 99%

See 2 more Smart Citations

Titanium Scaffolds by Direct Ink Writing: Fabrication and Functionalization to Guide Osteoblast Behavior

Vidal

Torres

Guillem-Marti

et al. 2020

Metals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to its diverse properties in regulating both osteogenic and supporting cell types, along with orchestrating the assembly of various other ECM proteins and GFs, incorporating FN into regenerative medicine applications is under intensive research (Xing et al, 2017;Cheng et al, 2018;Escoda-Francolí et al, 2018;Guillem-Marti et al, 2018;Parisi et al, 2019a;Sánchez-Garcés et al, 2020;Toffoli et al, 2020;Trujillo et al, 2020;Zhang et al, 2020). Lee et al (2019) recently developed an FN fusion protein, containing FNIII 9-10 domain and elastin like peptides (FN-ELP) to more reliably recapitulate mechanical properties of native ECM.…”

Section: Recent Advancements In Fibronectin-based Biomaterialsmentioning

confidence: 99%

Fibronectin in Fracture Healing: Biological Mechanisms and Regenerative Avenues

Klavert

Eerden

2021

Front. Bioeng. Biotechnol.

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The importance of extracellular matrix (ECM) proteins in mediating bone fracture repair is evident, and fibronectin (FN) has emerged as a pivotal regulator of this process. FN is an evolutionarily conserved glycoprotein found in all tissues of the body, and functions in several stages of fracture healing. FN acts as a three-dimensional scaffold immediately following trauma, guiding the assembly of additional ECM components. Furthermore, FN regulates cellular behavior via integrin-binding and growth factor-binding domains, promoting downstream responses including cell recruitment, proliferation and differentiation. Due to its diverse functions, the development of FN-based strategies to promote fracture healing is under intense research. In this review, we discuss the recent advancements in utilizing FN-based biomaterials, showing promise in tissue engineering and regenerative medicine applications.

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Guiding Fibroblast Activation Using an RGD‐Mutated Heparin Binding II Fragment of Fibronectin for Gingival Titanium Integration

Heras-Parets

Ginebra

Manero

et al. 2023

Adv Healthcare Materials

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The formation of a biological seal around the neck of titanium (Ti) implants is critical for ensuring integration at the gingival site and for preventing bacterial colonization that may lead to periimplantitis. This process is guided by activated fibroblasts, named myofibroblasts, which secrete extracellular matrix (ECM) proteins and ECM‐degrading enzymes resolving the wound. However, in some cases, Ti is not able to attract and activate fibroblasts to a sufficient extent, which may compromise the success of the implant. Fibronectin (FN) is an ECM component found in wounds that is able to guide soft tissue healing through the adhesion of cells and attraction of growth factors (GFs). However, clinical use of FN functionalized Ti implants is problematic because FN is difficult to obtain, and is sensitive to degradation. Herein, functionalizing Ti with a modified recombinant heparin binding II (HBII) domain of FN, mutated to include an Arg‐Gly‐Asp (RGD) sequence for promoting both fibroblast adhesion and GF attraction, is aimed at. The HBII‐RGD domain is able to stimulate fibroblast adhesion, spreading, proliferation, migration, and activation to a greater extent than the native HBII, reaching values closer to those of full‐length FN suggesting that it might induce the formation of a biological sealing.

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Recombinant Fibronectin Fragment III8-10/polylactic Acid Hybrid Nanofibers Enhance the Bioactivity of Titanium Surface

Abstract: Coating of Ti implants with hybrid CAS/PLA NFs may improve significantly their osseointegration potential.

Cited by 5 publications

References 57 publications

Titanium Scaffolds by Direct Ink Writing: Fabrication and Functionalization to Guide Osteoblast Behavior

Titanium Scaffolds by Direct Ink Writing: Fabrication and Functionalization to Guide Osteoblast Behavior

Fibronectin in Fracture Healing: Biological Mechanisms and Regenerative Avenues

Guiding Fibroblast Activation Using an RGD‐Mutated Heparin Binding II Fragment of Fibronectin for Gingival Titanium Integration

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