Recombinant Fusion Proteins for the Industrial Production of Disulfide Bridge Containing Peptides: Purification, Oxidation without Concatamer Formation, and Selective Cleavage

Döbeli, Heinz; Andres, Herbert; Breyer, Nicola; Draeger, Nicholas; Sizmann, Dorothea; Zuber, Maria Tomás; Weinert, Brian T.; Wipf, Beat

doi:10.1006/prep.1997.0844

Cited by 21 publications

(8 citation statements)

References 11 publications

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“…The NANP 19 antigen (synthesized by Hoffmann la Roche, Switzerland) (Döbeli et al . 1998) was diluted in phosphate‐buffered saline (PBS, pH 7.2) from which 100 μl (5 μg/ml) were applied per well of a 96‐well microtitre plate (Dynatech Immunolon II).…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

Plasmodium falciparum: use of a NANP₁₉ antibody‐test for the detection of infection in non‐immune travellers

Knappik

Peyerl‐Hoffmann

Jelı́nek

2002

Tropical Med Int Health

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SummaryCircumsporozoite (CS) antibodies are a reliable serological marker for the infection of Plasmodium falciparum. The purpose of this investigation was to construct and evaluate an enzyme-linked immunosorbent assay test for the detection of CS antibodies. While the sensitivity of the newly developed test reached 78%, the specificity was 99%. In addition, the optimized kit was used to test for infection with P. falciparum in 1903 travellers that were recruited from a prospective study for malaria chemoprophylaxis. Sixty-six of the 1903 patients (3.5%) showed elevated CS antigen antibody titres. However, seroconversion could only be demonstrated in 18 (0.95%) patients. Among those seroconverting, there was a significantly higher percentage of male travellers (1.28%) than female travellers (0.56%). Positive reactions were more frequent among returnees from West and East Africa (1.49 and 1.14%, respectively) than among those from other endemic areas, e.g. South America (n ¼ 0). Despite its limited sensitivity, this newly developed kit for CS antibody testing may be a valuable tool for the estimation of the risk for travellers in malarious regions to acquire an infection with P. falciparum. It may also be useful for the determination of the efficacy of malaria chemoprophylaxis for inhibiting outbreak of disease.

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Section: Patients Materials and Methodsmentioning

confidence: 99%

Plasmodium falciparum: use of a NANP₁₉ antibody‐test for the detection of infection in non‐immune travellers

Knappik

Peyerl‐Hoffmann

Jelı́nek

2002

Tropical Med Int Health

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“…The primary obstacles are associated with the toxicity of these proteins to the host cells and susceptibility to the intracellular degradation by proteolytic enzymes [168][169][170][171]. Furthermore, 30-45 amino acid long defensins are at the very lower limit of protein size required for the stable expression [172]. Additional difficulties arise from the high content of basic and cysteine residues, resulting often in very low expression levels and incorrect folding due to the mismatches during formation of disulfides.…”

Section: Requirements Limitations Obstaclesmentioning

confidence: 99%

Human Defensins: Synthesis and Structural Properties

Pazgier

Li²,

Lu³

et al. 2007

CPD

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Defensins are small, beta-sheet-rich, cationic peptides found in many organisms. All defensins have amphiphilic properties, which are central for antimicrobial activities of the proteins. The human genome encodes many defensin-line molecules, of which 10 have been characterized. Molecules of all known human defensins are stabilized by three intramolecular disulfide bonds arranged in a conserved pattern. To date, studies of human defensins indicate that these proteins are involved in various biological processes associated primarily with defensive and regulatory responses to infections by pathological agents. A comprehensive understanding of the multiple roles played by defensins within the immune system is greatly increased by reviewing the results of detailed structural studies. Emerging structural data, derived by the X-ray crystallography and the NMR spectroscopy in solution combined with functional studies; allow a rational understanding of the different activities of defensins and the mechanisms controlling these activities. Due to their well-established antimicrobial properties, defensins are also being investigated for their potential as therapeutics agents. Recently, increased effort has been focused on studies of the immunoregulatory properties of defensins, associated with their ability to bind and activate the G(i)-protein-coupled seven-transmembrane receptors. Comprehensive studies of defensins require development of simple, efficient, and inexpensive methods to generate these proteins and their derivatives in correctly folded form. This review highlights the current status of the sample generation methods, structural studies, and the structure-function relationships for human defensins.

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“…[11][12][13] The structure of a protease-resistant fragment of gp41 from HIV-1, which forms the hexa-helix bundle, has been investigated 3,4,5,14 as well as the structure of gp41 from SIV. [15][16][17] Furthermore, a soluble five-helix construct has been designed in which the N and C-helices of gp41 are connected by artificial linkers.…”

Section: Introductionmentioning

confidence: 99%

Functional Solubilization of Aggregation-prone HIV Envelope Proteins by Covalent Fusion with Chaperone Modules

Schölz

Schaarschmidt

Engel

et al. 2005

Journal of Molecular Biology

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Recombinant Fusion Proteins for the Industrial Production of Disulfide Bridge Containing Peptides: Purification, Oxidation without Concatamer Formation, and Selective Cleavage

Cited by 21 publications

References 11 publications

Plasmodium falciparum: use of a NANP₁₉ antibody‐test for the detection of infection in non‐immune travellers

Plasmodium falciparum: use of a NANP₁₉ antibody‐test for the detection of infection in non‐immune travellers

Human Defensins: Synthesis and Structural Properties

Functional Solubilization of Aggregation-prone HIV Envelope Proteins by Covalent Fusion with Chaperone Modules

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Recombinant Fusion Proteins for the Industrial Production of Disulfide Bridge Containing Peptides: Purification, Oxidation without Concatamer Formation, and Selective Cleavage

Cited by 21 publications

References 11 publications

Plasmodium falciparum: use of a NANP19 antibody‐test for the detection of infection in non‐immune travellers

Plasmodium falciparum: use of a NANP19 antibody‐test for the detection of infection in non‐immune travellers

Human Defensins: Synthesis and Structural Properties

Functional Solubilization of Aggregation-prone HIV Envelope Proteins by Covalent Fusion with Chaperone Modules

Contact Info

Product

Resources

About

Plasmodium falciparum: use of a NANP₁₉ antibody‐test for the detection of infection in non‐immune travellers

Plasmodium falciparum: use of a NANP₁₉ antibody‐test for the detection of infection in non‐immune travellers