Recombinant goose-type lysozyme in channel catfish: Lysozyme activity and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

Pridgeon, Julia W.; Klesius, Phillip H.; Dominowski, Paul J.; Yancey, Robert J.; Kievit, Michele S.

doi:10.1016/j.fsi.2013.08.015

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…High expression of AMGs in the spleen, skin, muscle, liver and kidney of a variety of fish, as well as in certain other tissues, is encouraging and indicative of potential disease resistance in the current investigation. Pridgeon et al (2013a) found that chicken-type lysozyme showed higher expression levels in the spleen and posterior kidney after A. hydrophila infection, but a lower level was represented in blood, skin and liver. Besides, the mRNA of GRN1 was abundantly expressed in the spleen, liver, muscle and kidney of GRN-41 transgenic zebrafish, followed by the skin, gill and head (Wu et al, 2018).…”

Section: Discussionmentioning

confidence: 91%

“…The current meta-analysis extracted 74 figures from eight studies, including line charts and histograms (Supplementary S3). The 540 effect sizes (k = 14 for CFU, k = 3 for LYA, k = 50 for SCR, k = 42 for TGE, and k = 431 for IRGE) from 18 studies spanning 8 fish species and 12 diseases were included in the recruited dataset (Dunham et al, 2002; Sarmasik et al, 2002; Zhong et al, 2002; Mao et al, 2004; Yazawa et al, 2006; Lin et al, 2009; Hsieh et al, 2010; Peng et al, 2010; Pan et al, 2011; Chiou et al, 2014; Pridgeon et al, 2013ab; Lee et al, 2013; Lin et al, 2016; Wu et al, 2018; Su et al, 2018; Elaswad et al, 2019; Simora et al, 2020). In total, 15 AMGs were integrated into the fish genome in an attempt to improve disease resistance via genetic engineering (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Although the most intuitive advantage of AMG-integrated fish is a significantly higher survival rate when they are infected by pathogens. An increasing number of studies indicated that underlying factors like decreased colony-forming unit (CFU) of bacteria, enhanced enzyme activity, increased expressions of AMGs and immune-related genes contributed to improving the survival rate in individuals harboring foreign AMGs (Pridgeon et al, 2013ab; Lo et al, 2014; Simora et al, 2020). For example, tail tissue of hepcidin-transgenic zebrafish ( Danio rerio ) and convict cichlid ( Archocentrus nigrofasciatus ) exhibited significant reduction in CFU against Vibrio vulnificus compared to wild-type fish (Hsieh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Harnessing antimicrobial peptide genes to expedite disease-resistant enhancement in aquaculture: Transgenesis and genome editing

Wang

Cheng

2023

Preprint

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Numerous studies have demonstrated that genome editing and transgenesis by integrating vector-engineered antimicrobial peptide genes (AMGs) is effective to modulate the fish's innate immune system. To generalize the knowledge of AMG application in aquaculture, here we recruited 540 data entries from a pool of empirical studies, which included 18 peer-reviewed publications and spanning 12 diseases. We systematically re-processed and re-analyzed these data by harnessing a cross-disease meta-analysis. On aggregate, AMG-genetic engineering aimed at enhancing disease resistance was shown to decrease the number of colony-forming units of bacteria, improve lysozyme activity, increase the post-infection survival rates, and alter the expression of AMGs and immune-related genes in aquatic animals. Furthermore, the AMG-pathogen combating activity was triggered within two hours after infection and lasted 48 hours, and the overexpression of AMGs was dominant in the spleen and skin, followed by the kidney and liver during this period. Typically, regardless of the type of AMGs, the synergistic expression of AMGs with IL, IKβ, TGFβ, C3b and TLR in AMG-integrated fish contributed to activating inflammatory/immune responses against pathogens. In addition, innovative CRISPR/Cas9-mediated systems enabling the site-directed knock-in of foreign genes at multiple loci were presented and prospected for disease-resistant enhancement in combination with other favorable fish-producing traits, including fast-growing, sterility, and enriched fatty acid. Altogether, our findings indicated that AMGs as transgenes have substantial potential to modulate the fish's innate immune system and accelerate disease-resistant enhancement combined with genetic engineering.

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Section: Discussionmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Harnessing antimicrobial peptide genes to expedite disease-resistant enhancement in aquaculture: Transgenesis and genome editing

Wang

Cheng

2023

Preprint

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“…In addition to BLF and cecropin, a recombinant hepcidin peptide was added to grass carp feed formula, and protection against F. columnare was achieved via regulation of the iron distribution and immune gene expression 157 . Pridgeon et al 158,159 developed two recombinant plasmids containing chicken‐type (pcDNA‐Lys‐c) and goose‐type (pcDNA‐Lys‐g) lysozyme, which can be used as novel immunostimulants to protect channel catfish against A. hydrophila infection. For these plasmid DNA immunostimulants, Pridgeon's team delivered these plasmid DNA immunostimulants to the fish via intraperitoneal injection and the challenge experiment revealed that these novel immunostimulants offered 100% protection against A. hydrophila at 2 days post injection 158,159 …”

Section: Application Of Exogenous Amps/amgs In Fishmentioning

confidence: 99%

Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

Wang

Dunham

2022

Reviews in Aquaculture

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Antimicrobial peptides (AMPs), also known as host defence peptides, are evolutionarily ancient defensive weapons that can combat infections caused by pathogens. Fish‐derived AMPs have shown microbicidal properties, host immunomodulatory responses, and are induced by a variety of factors, especially pathogenic infection and abiotic stress, which may activate downstream signalling pathways to initiate the expression of antimicrobial peptide genes. Large‐scale genome sequencing has been applied in many aquatic animals, providing databases for the systematic identification of putative AMPs in these species. Recently, the whole genome and the proteome of channel catfish (Ictalurus punctatus) were released; by taking advantage of the antimicrobial peptide database and combining the proteome of catfish, we established a bioinformatic analysis pipeline to identify catfish AMP repertoire. This review introduces an effective method for the identification and development of putative AMPs based on protein datasets, and summarizes the structural properties, immunomodulation and molecular responses in vivo of catfish AMPs. In addition, the evaluation of the antibacterial activity of synthetic or isolated AMPs in vitro, and the applications of AMPs in fish via genetic engineering and clustered regularly interspaced short palindromic repeats associated proteins system (CRISPR/Cas9) were presented, which supports antimicrobial research and aquaculture therapy. The summary and comparison of these AMPs will enhance our understanding and their cross‐species applications. Combined with current genetic engineering and genome editing techniques, it is possible to promote the development of immune processes to protect valuable aquatic animals from infectious diseases.

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“…The infection of vAh significantly induced transcription of apolipoprotein A1 [ 54 ], chicken-type lysozyme [ 55 ], G-protein coupled receptor 18 [ 56 ], and goose-type lysozyme [ 57 ] in kidney, liver, and other tissues of channel catfish. The lysozymes were expressed in the E. coli expression system and exhibited high lytic activity against the pathogen.…”

Section: Bacterial Pathogens Commonly Observed In Catfish Culturementioning

confidence: 99%

A Review of Bacterial Co-Infections in Farmed Catfish: Components, Diagnostics, and Treatment Directions

Wise

LaFrentz

Kelly

et al. 2021

Animals

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Catfish production is a major aquaculture industry in the United States and is the largest sector of food fish production. As producers aim to optimize production yields, diseases caused by bacterial pathogens are responsible for high pond mortality rates and economic losses. The major bacterial pathogens responsible are Edwardsiella ictaluri, Aeromonas spp., and Flavobacterium columnare. Given the outdoor pond culture environments and ubiquitous nature of these aquatic pathogens, there have been many reports of co-infective bacterial infections within this aquaculture sector. Co-infections may be responsible for altering disease infection mechanics, increasing mortality rates, and creating difficulties for disease management plans. Furthermore, proper diagnoses of primary and secondary pathogens are essential in ensuring the correct treatment approaches for antimicrobials and chemical applications. A thorough understanding of the interactions and infectivity dynamics for these warm water bacterial pathogens will allow for the adoption of new prevention and control methods, particularly in vaccine development. This review aims to provide an overview of co-infective pathogens in catfish culture and present diagnostic case data from Mississippi and Alabama to define prevalence for these multiple-species infections better.

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Recombinant goose-type lysozyme in channel catfish: Lysozyme activity and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

Cited by 11 publications

References 33 publications

Harnessing antimicrobial peptide genes to expedite disease-resistant enhancement in aquaculture: Transgenesis and genome editing

Harnessing antimicrobial peptide genes to expedite disease-resistant enhancement in aquaculture: Transgenesis and genome editing

Genome‐wide identification of catfish antimicrobial peptides: A new perspective to enhance fish disease resistance

A Review of Bacterial Co-Infections in Farmed Catfish: Components, Diagnostics, and Treatment Directions

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