Recombinant HMG1 Protein Produced in
            <i>Pichia pastoris</i>
            : A Nonviral Gene Delivery Agent

Mistry, A; Falciola, Luca; Monaco, Lucía; Tagliabue, Roberta; Acerbis, Giulia; Knight, Andrew M.; Harbottle, Richard P.; Soria, Marco R.; Bianchi, Marina; Coutelle, Charles; Hart, Stephen L.

doi:10.2144/97224rr01

Cited by 45 publications

(25 citation statements)

References 24 publications

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“…Endogenous proteins such as members of the high mobility group proteins (HMG) and histones have also been used as pDNA delivery systems as such [166,167] or in combination with conventional lipids and polymers [168] to increase transfection efficiency [169]. These proteins were able to enhance pDNA nuclear translocation and transfection efficiency to some extent, most likely due to their innate DNA binding and condensing properties, as well as their intrinsic NLSs.…”

Section: Nonviral Chromatin Tethering Factorsmentioning

confidence: 99%

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Symens

Soenen

Rejman

et al. 2012

Advanced Drug Delivery Reviews

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Section: Nonviral Chromatin Tethering Factorsmentioning

confidence: 99%

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Symens

Soenen

Rejman

et al. 2012

Advanced Drug Delivery Reviews

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“…Tissue-specific gene transfer could be achieved naturally and effectively through the cell specificity of virus receptors. 46 However, there is a risk of vector toxicity through viral infection of the host cells. Also, the limited size of transgenes often presents a serious obstacle.…”

Section: Il-4 Antagonistic Mutant Dna Inhibits Allergic Airway Inflammentioning

confidence: 99%

A single administration of interleukin-4 antagonistic mutant DNA inhibits allergic airway inflammation in a mouse model of asthma

et al. 2003

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Interleukin 4 (IL-4) is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. These mechanisms are thought to play a crucial role in the pathogenesis of the allergic airway inflammation observed in asthma. In the present study, we examined the anti-inflammatory effects of DNA administration of murine IL-4 mutant Q116D/Y119D (IL-4 double mutant, IL-4DM), which binds to the IL-4 receptor a and is an antagonist for IL-4. Immunization of BALB/c mice with alum-adsorbed ovalbumin (OVA) followed by aspiration with aerosolized OVA resulted in the development of allergic airway inflammation. A single administration of IL-4DM DNA before the aerosolized OVA challenge protected the mice from the subsequent induction of allergic airway inflammation. Serum IgE level and extent of eosinophil infiltration in bronchoalveolar lavage (BAL) from IL-4DM DNA-administered mice were significantly lower than those in BAL from control plasmid-immunized mice. In our study, IL-4 or IL-4 mutants were not detected in sera from mice that had received a single administration of IL-4DM DNA. The results of this study provide evidence for the potential utility of IL-4 mutant antagonist DNA inoculation as an approach to gene therapy for asthma.

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“…Also, attachment to cellular DNA binding proteins including histones 14 and high-mobility group (HMG) proteins 15 , as well as DNA containing the Simian Virus 40 (SV40) promoter and origin of replication 16 Interestingly, several groups have observed that the expression efficiencies of cationic lipid-, polymerand peptide-based transfection reagents were two-to several hundred-fold higher in dividing as compared to non-dividing cells , have been investigated. These attempts at improving the transport of DNA to the nucleus have however met with limited success.…”

mentioning

confidence: 99%

Nuclear Inclusion of Nontargeted and Chromatin-Targeted Polystyrene Beads and Plasmid DNA Containing Nanoparticles

et al. 2011

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ABSTRACT. The nuclear membrane is one of the major cellular barriers in the delivery of plasmid DNA (pDNA). Cell division has a positive influence on the expression efficiency since at the end of mitosis, pDNA or pDNA containing complexes near the chromatin are probably included by a random process in the nuclei of the daughter cells. However, very little is known about the nuclear inclusion of nanoparticles during cell division. Using the Xenopus nuclear envelope reassembly (XNER) assay, we found that the nuclear enclosure of nanoparticles was dependent on size (with 100 nm and 200 nm particles being better included than the 500 nm ones) and charge (with positively charged particles being better included than negatively charged or poly-ethyleneglycolated (PEG-ylated) ones) of the 2 beads. Also, coupling chromatin-targeting peptides to the polystyrene beads or pDNA complexes improved their inclusion by 2-to 3-fold. Upon microinjection in living HeLa cells, however, nanoparticles were never observed in the nuclei of cells post-division but accumulated in a specific perinuclear region, which was identified as the lysosomal compartment. This indicates that nanoparticles can end up in the lysosomes even when they were not delivered through endocytosis. To elucidate if the chromatin binding peptides also have potential in living cells, this additional barrier first has to be tackled, since it prevents free particles to be present near the chromatin at the moment of cell division.

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Recombinant HMG1 Protein Produced in Pichia pastoris : A Nonviral Gene Delivery Agent

Abstract: ABSTRACT

Cited by 45 publications

References 24 publications

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

A single administration of interleukin-4 antagonistic mutant DNA inhibits allergic airway inflammation in a mouse model of asthma

Nuclear Inclusion of Nontargeted and Chromatin-Targeted Polystyrene Beads and Plasmid DNA Containing Nanoparticles

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