Recombinant human gelsolin promotes the migration of human articular cartilage chondrocytes by regulating gene expression in vitro

Feldt, Jessica; Welss, Jessica; Schropp, Verena; Gelse, Kolja; Tsokos, Michael; Paulsen, Friedrich

doi:10.1016/j.ocarto.2020.100124

Cited by 3 publications

(7 citation statements)

References 34 publications

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“…In RA studies, GSN injection has already been shown to have a positive anti-inflammatory and chondroprotective effect [40]. Our own recently published results showed similar positive results of GSN in an OA in vitro model, as well as a significant positive effect on human primary chondrocyte survival and migration [36]. Since GSN has also been detected in synovial fluid, it is reasonable to assume that GSN is produced by synoviocytes [41].…”

Section: Introductionmentioning

confidence: 71%

“…In addition to joints, GSN has been detected in lung, heart, skeletal muscle and a number of other tissues [42]. For chondrocytes of articular cartilage, GSN has been shown to have a protective effect in simulating regenerative processes and differentiation in cartilage [36,43]. Therefore, the presence of GSN in synovial fluid is important for maintaining the health of cartilage and joint.…”

Section: Discussionmentioning

confidence: 99%

“…We know from previous studies that GSN is expressed in chondrocytes of articular cartilage. However, the concentration of GSN produced by chondrocytes in the synovial fluid cannot be explained [36]. It was therefore reasonable to assume that synoviocytes, as the inner layer of the synovial membrane, are involved in the production and secretion of GSN into the synovial fluid.…”

Section: Discussionmentioning

confidence: 99%

“…GSN is an actin-binding protein involved in cell mobility, cell shape, metabolism and wound healing processes [34,35]. Three isoforms are known today, with the plasmatic form (pGSN) being the most common, involved in various immune system processes as well as wound healing [34,36]. The structural data that have been established form the basis of the functional understanding of gelsolin, which have recently been summarized in more detail [34].…”

Section: Introductionmentioning

confidence: 99%

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Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis

et al. 2022

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Gelsolin (GSN) is an actin-binding protein involved in cell formation, metabolism and wound closure processes. Since this protein is known to play a role in arthritis, here we investigate how the synovial membrane with its specific synoviocytes contributes to the expression of GSN and how the amount of GSN expressed is modulated by different types of arthritis. Synovial membranes from adult healthy subjects and patients with rheumatoid arthritis (RA) and osteoarthritis (OA) are analyzed by immunofluorescence, Western blot and ELISA. Macrophage-like synoviocytes (MLS) and fibroblast-like synoviocytes (FLS) were isolated, cultured and analyzed for their potential to produce and secrete GSN. In addition, the GSN concentrations in the synovial fluid of various forms of arthritis are determined by ELISA. GSN is produced by the healthy and arthritic synovial membranes. Both forms of synoviocytes (MLS and FLS) release GSN. The results show that there is a significant reduction in GSN in the synovial fluid in adult patients with OA. This reduction is also detectable in adult patients with RA but is not as evident. In juvenile arthritis, there is a slight increase in GSN concentration in the synovial fluid. This study shows that primary MLS and FLS express GSN and that these cells, in addition to articular chondrocytes, contribute to GSN levels in synovial fluid. Furthermore, GSN concentrations are modulated in different types of arthritis. Further studies are needed to fully understand how GSN is involved in joint homeostasis.

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Section: Introductionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis

et al. 2022

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“…32 Besides, the pGSN treatment also modulates the expression of cartilage ECM-related genes, providing an environment to support wound healing. 32 Nevertheless, pGSN-mediated cytokine production and release are currently far from being comprehensively understood. In particular, the biological function and physiological significance of interaction between pGSN and FN have never been elucidated.…”

Section: The Roles Of Pgsn In Inflammatory Responses and Wound Healingmentioning

confidence: 99%

Emerging roles of plasma gelsolin in tumorigenesis and modulating the tumor microenvironment

Hsieh

Wang

2022

The Kaohsiung J of Med Scie

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The protein expression of gelsolin, an actin scavenger controlling cytoskeletal remodeling, cell morphology, differentiation, movement, and apoptosis, has been found to be significantly decreased in several pathological conditions including neurodegenerative diseases, inflammatory disorders, and cancers. Its extracellular isoform, called plasma gelsolin (pGSN), is one of the most abundant plasma proteins in the circulation, and has emerged as a novel diagnostic biomarker for early disease detection. Current evidence reveals that gelsolin can function as either an oncoprotein or a tumor suppressor depending on the carcinoma type. Interestingly, recent studies have shown that pGSN is also involved in immunomodulation, revealing the multifunctional roles of pGSN in tumor progression. In this review, we discuss the current knowledge focusing on the roles of gelsolin in inflammation and wound healing, cancers, and tumor microenvironment. Future prospects of pGSN related studies and clinical application are also addressed.

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Unravelling the Basic Calcium Phosphate crystal-dependent chondrocyte protein secretome; a role for TGF-β signaling

Stassen¹,

Akker²,

Surtel³

et al. 2023

Osteoarthritis and Cartilage

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Recombinant human gelsolin promotes the migration of human articular cartilage chondrocytes by regulating gene expression in vitro

Cited by 3 publications

References 34 publications

Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis

Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis

Emerging roles of plasma gelsolin in tumorigenesis and modulating the tumor microenvironment

Unravelling the Basic Calcium Phosphate crystal-dependent chondrocyte protein secretome; a role for TGF-β signaling

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