Recombinant Human Growth Hormone Promotes Burn Wound Repair by Regulating Vascular Endothelial Growth Factor and Micro Vessel Density

Abstract

To explore the effect of recombinant human growth hormone on burn wound healing by regulating vascular endothelial growth factor and micro vessel density. Sixty rats were randomly divided into control group (n=20) (group A), low dose recombinant human growth hormone intervention group (n=20) (group B) and high dose recombinant human growth hormone intervention group (n=20) (group C). After the deep II burn wounds were formed in all rats, 1.0 ml saline was injected into the back burn wounds in the group A, 1.0 ml of 0.2 IU/ ml recombinant human growth hormone saline solution was injected into the back burn wounds in the low dose recombinant human growth hormone intervention group, and 0.4 IU/ml recombinant human growth hormone saline solution was injected into the back burn wounds in the group C every day. The time of scab removal and hair growth, vascular endothelial growth factor expression, micro vessel density, oxidative stress index and inflammation index were compared in each group. Rats in group B showed shorter decrustation and hair growth times than those in group A, while rats in group C showed shorter decrustation and hair growth times. In group A, vascular endothelial growth factor expression was higher than in group B, and in group C, vascular endothelial growth factor expression was higher than in group B. In group B, the level of micro vessel density was higher than in group A, and in group C, it was higher than in group A. Superoxide dismutase levels in the group B were higher than those in the group A, and malondialdehyde levels in the group C were higher than those in the group B, while malondialdehyde levels in the group C were lower than those in the group A. As compared to group A, the levels of interleukin-6, tumor necrosis factor-alpha, and interleukin-1 alpha were reduced in group B, while in group C they were decreased. Recombinant human growth hormone can promote burn wound repair by regulating vascular endothelial growth factor and micro vessel density.