2007
DOI: 10.1128/aem.02091-06
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RecombinantSaccharomyces cerevisiaeStrain Expressing a Model Cytochrome P450 in the Rat Digestive Environment: Viability and Bioconversion Activity

Abstract: An innovative "biodrug" concept, based on the oral administration of living recombinant microorganisms, has recently emerged for the prevention or treatment of various diseases. An engineered Saccharomyces cerevisiae strain expressing plant P450 73A1 (cinnamate-4-hydroxylase [CA4H] activity) was used, and its survival and ability to convert trans-cinnamic acid (CIN) into p-coumaric acid (COU) were investigated in vivo. In rats, the recombinant yeast was resistant to gastric and small intestinal secretions but … Show more

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Cited by 14 publications
(8 citation statements)
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“…Gas production is an indicator of an active fermentation (Pell and Schofi eld, 1993) and stability in gas production from day 2 to day 10 might suggest a stability in the fermentation and the adaptation of the microbiota within the Cositec. At the steady state, the yeast survival rate was 1% (results not shown), which is in accordance with previous studies (rat model) showing that the major loss of S. cerevisiae happened in the large intestine rather than in the stomach and small intestine (Garrait et al, 2007). There was an Table 1.…”
Section: Resultssupporting
confidence: 91%
“…Gas production is an indicator of an active fermentation (Pell and Schofi eld, 1993) and stability in gas production from day 2 to day 10 might suggest a stability in the fermentation and the adaptation of the microbiota within the Cositec. At the steady state, the yeast survival rate was 1% (results not shown), which is in accordance with previous studies (rat model) showing that the major loss of S. cerevisiae happened in the large intestine rather than in the stomach and small intestine (Garrait et al, 2007). There was an Table 1.…”
Section: Resultssupporting
confidence: 91%
“…The synthesis of VLPs using the yeast expression system has several advantages. S. cerevisiae is a GRAS (generally recognized as safe) microorganism, free of toxins hazardous to human health [ 52 , 53 ]. The preparation of products produced in yeast for human use is well established.…”
Section: Discussionmentioning
confidence: 99%
“…With the ultimate goal of substrate conversions, coexpression of P450 and CPR can be beneficial, as deprivation of a redoxpartner is unlikely. Garrait et al [ 65 ] engineered a S. cerevisiae strain, for coexpression of plant P450 73A1 and CPR, which allowed conversion of cinnamic acid to coumaric acid. Nazir et al [ 66 ] constructed a library of 121 isoforms of cytochrome P450 monooxygenases from Aspergillus oryzae , and coexpressed them together with NADPH-cytochrome reductase in S. cerevisiae to find new catalytic functions.…”
Section: Strategies For P450 Production In Microbialsmentioning
confidence: 99%