Recombinant infectious hematopoietic necrosis virus expressing infectious pancreatic necrosis virus VP2 protein induces immunity against both pathogens

Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐GN438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout

“…At the same time, glycosylation and deglycosylation of the G proteins on the virus surface directly impact the pathogenicity and the immunogenicity of the virus (Lorenzen et al, 2002;Ostrowski et al, 2002;Pletnev et al, 1993;Verjan et al, 2008). We have tested that the recombinant virus (rIHNV-N438A) could lead to a reduction in the lethal rate of rainbow trout (Guo et al, 2018).…”

“…CHSE‐214 cells infected with rIHNV‐N438A‐ΔNV‐VP2 for 48 hr, the cell culture medium was discarded and washed with pre‐chilled PBS, then collected by low centrifugation after scraping the cells. Western blot analysis was performed as described previously, and the immunoblots were developed using rabbit anti‐IPNV VP2 as the primary antibody and followed by HRP‐conjugated goat anti‐rabbit (Guo et al, ).…”

“…The plasmid of pIHNV‐N438A‐ΔNV‐VP2 or pIHNV‐N438A‐ΔNV‐EGFPP was transfected into CHSE‐214 cells with five kinds of helper plasmids, respectively. Transfection process as described by Guo et al () was used. The cell monolayer was observed for the development of virus‐induced cytopathic effect (CPE), and then, the supernatant was harvested and clarified for further processing of the recombinant viruses.…”

“…Western blot analysis was performed as described previously, and the immunoblots were developed using rabbit anti-IPNV VP2 as the primary antibody and followed by HRP-conjugated goat anti-rabbit (Guo et al, 2018).…”

“…These epitopes are capable of eliciting the production of viral neutralizing antibodies against IPNV (Frost et al, 1995;Munang'Andu & Evensen, 2015). Vaccines encoding the recombinant VP2 gene had been shown to be sufficient to induce a protective immune response (Ahmadivand, Soltani, Behdani et al, 2017;Ahmadivand, Soltani, Mardani et al, 2017;Ballesteros, Saint-Jean, Encinas, Perez-Prieto, & Coll, 2012;Guo et al, 2018). A previous study showed that the truncated VP2 protein (147-307 amino acids) is capable of inducing an anti-IPNV antibody response with antibody titres similar to those for whole IPNV (Labus et al, 2001).…”