Recombinant Interferon Alpha-2b is a High-Affinity Antigen for Type 1 Diabetes Autoantibodies

Khan, Wahid Ali

doi:10.1016/j.jcjd.2016.10.001

Cited by 8 publications

(5 citation statements)

References 39 publications

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“…Remaining islet cells often express class II MHC molecules, an effect of local production of INF-γ by the T cells [33,73,79]. The onset of young age of this disease may be associated with upregulation of growth factors, especially GM-CSF and IL-7.…”

Section: Cytokines and Autoantibodiesmentioning

confidence: 99%

Cytokines and Interferons: Types and Functions

Ferreira¹,

Borba²,

Bonetti³

et al. 2019

Autoantibodies and Cytokines

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This chapter aims to describe and review the main important cytokines types (notably interferons), including their biological activities, functions and structures. As a high number of molecules are available, synthesis of the most important cytokines, including tumor factor necrosis, interferons and interleukins will be presented. Here we also describe the relationships between those cytokines with some autoimmune diseases that are promoted by them.

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Section: Cytokines and Autoantibodiesmentioning

confidence: 99%

Cytokines and Interferons: Types and Functions

Ferreira¹,

Borba²,

Bonetti³

et al. 2019

Autoantibodies and Cytokines

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“…These metabolites lead to the generation of ROS that can damaged DNA and make DNA adducts [49]. Once DNA get damaged it alters its antigenicity leading to the generation of autoanti- bodies in autoimmune diseases [15][16][17][18][19][20][21][22][23][24][25][26]44]. P450-mediated hydroxylation also produced 16α-hydroxyestrone metabolites that exert its effect through binding to its receptor [51].…”

Section: Discussionmentioning

confidence: 99%

“…Recent study showed that 16α-hydroxyestrone is responsible for causing breast cancer [15]. Infect, these estrogen metabolites play an important role in cancer as well as in autoimmune diseases [15][16][17][18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

16α-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients

Khan¹,

Khan²

2021

JOMH

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Background and objectives: Elevated levels of 16α-hydroxyestrone (16α-OHE1) have been linked to increased risk of prostate cancer (PC) and estrogen receptor (ER) had been expressed in prostate tissue but the combined effect of 16α-OHE1 and ER (α) is lacking. We investigated the binding specificity of antibodies from PC with 16α-OHE1-ER complex in the sera from PC patients. Materials and methods: The antibodies in the serum from 60 PC patients and 40 control subjects were evaluated from ELISA (direct binding and competition) and quantitative precipitin titration. Competition ELISA was also used to estimates 16α-OHE1 concentration and 2-hydroxyestrone (2-OHE1)/16α-OHE1 ratio in PC patients. Results: Antibodies from PC patients demonstrate high binding to 16α-OHE1-ER in comparison to ER (p < 0.05) or 16α-OHE1 (p < 0.001). The relative affinity of PC IgG was found to be high for 16α-OHE1-ER (1.19 × 10−7 M) as compared to ER (1.45 × 10−6 M) or 16α-OHE1 (1.13 × 10−6 M). Conclusion: High affinity of PC IgG with 16α-OHE1-ER might explain the possible antigenic role and 16α-OHE1-ER acted as high affinity antigen for antibodies from PC. The interaction between 16α-OHE1 and ER makes a complex in the prostate tissues and this may generate antibodies against this complex in the cancer patients.

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“…The immune complexes between EC IgG and antigens (16α‐OHE 1 ‐ERα, ERα and 16α‐OHE 1 ) were prepared as mentioned previously 21 . Briefly, a fixed amount of IgG (100 μg) was incubated with different amounts of antigens (0–100 μg) in a total volume of 400 μL of the reaction mixture.…”

Section: Methodsmentioning

confidence: 99%

Endometrial cancer patients have high affinity antibodies for estrogen metabolite–receptor aggregate: A potential biomarker for EC

Khan

Alsamghan

Khan

2020

J of Obstet and Gynaecol

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Aim: Elevated levels of 16α-hydroxyestrone (16α-OHE 1) have been described in endometrial cancer (EC) and estrogen receptors (ER) expressed in endometrial tissue, but research on their combined role is lacking. We aimed to investigate the affinity and binding specificity of EC antibodies against the 16α-OHE 1-ERα aggregate in the serum of EC patients. Specificities of EC antibodies were also evaluated according to various clinical characteristics found in these cancer patients. Methods: The binding specificity and affinity of EC antibodies against 16α-OHE 1-ERα in the serum of 120 EC patients were evaluated by direct binding and competition ELISA and quantitative precipitation titration. Binding of EC antibodies was also determined according to various clinical characteristics in EC patients through competition ELISA. Results: Antibodies from EC patients demonstrated high recognition of 16α-OHE 1-ERα compared to ERα (P < 0.05) or 16α-OHE 1 (P < 0.001). The relative affinity of EC IgG was 1.49 × 10 −7 M, 1.34 × 10 −6 M and 1.13 × 10 −6 M for 16α-OHE 1-ERα, ERα and 16α-OHE 1 , respectively. Several factors, such as obesity, postmenopausal status, use of hormonal therapy, ER and progesterone receptor (PR) status, low 2-OHE 1 /16α-OHE 1 ratio, chemotherapy and hypertension, augment the production of antibodies against 16α-OHE 1-ERα in EC patients. Conclusion: 16α-OHE 1-ERα is a high-affinity antigen for EC antibodies in the serum of EC patients and might function as a biomarker for this disease. Furthermore, several factors enhanced the production of antibodies against 16α-OHE 1-ERα in the sera of these EC patients.

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Recombinant Interferon Alpha-2b is a High-Affinity Antigen for Type 1 Diabetes Autoantibodies

Cited by 8 publications

References 39 publications

Cytokines and Interferons: Types and Functions

Cytokines and Interferons: Types and Functions

16α-hydroxyestrone and its receptor complex: high affinity antigen for antibodies from prostate cancer patients

Endometrial cancer patients have high affinity antibodies for estrogen metabolite–receptor aggregate: A potential biomarker for EC

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