Recombinant Interleukin-1 Triggers the Increase of Circulating Fibronectin Level in Rats

Hagiwara, Toshiyuki; Kono, Ichiro; Nemoto, Koji; Kashiwagi, Hirokazu; Onozaki, Kikuo

doi:10.1159/000234978

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…We have observed that during the process of monocyte transmigration into the subendothelial space of the coculture the cells acquire the typical appearance of spread monocytes suggesting the activation of these cells (unpublished observations, and reference 61). Injection of IL-1 into rats has been reported to produce elevated serum levels offibronectin (62). Leukocyte interleukins have also been reported to induce cultured EC to produce a highly organized pericellular matrix (63).…”

Section: Discussionmentioning

confidence: 89%

Interaction of monocytes with cocultures of human aortic wall cells involves interleukins 1 and 6 with marked increases in connexin43 message.

Navab¹,

Liao²,

Hough³

et al. 1991

J. Clin. Invest.

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Medium from cocultures of human aortic endothelial cells (HAEC) and smooth muscle cells (HASMC) taken from the same donor contained approximately two-to fourfold more macrophage colony-stimulating factor, granulocyte/macrophage colony-stimulating factor, and up to 5.1-fold more transforming growth factor fi than could be accounted for by the sum of the activities of media from equivalent numbers of HAEC and HASMC cultured separately. After pulse labeling, immunoprecipitated I35Slfibronectin and I'4Cicollagen were also found to be substantially increased in the coculture compared to the sum of HAEC and HASMC cultured separately. The cocultivation of HAEC and HASMC resulted in a 2.7-fold increase in connexin43 messenger RNA. When direct physical contact between HAEC and HASMC was prevented by a membrane that was permeable to medium, the levels of ['Sfibronectin and 114Cjcollagen in the coculture were significantly reduced. Monocytes cultured alone contained low levels of PI5Slfibronectin and ['4Cjcollagen but when added to the coculture there was up to a 22-fold increase in I3Slfibronectin and a 1.9-fold increase in [14Cjcollagen compared to the coculture alone. The increase in fibronectin was prevented in the presence of neutralizing antibody to interleukin 1 and antibody to interleukin 6 by 45% and 67%, respectively. Addition of monocytes to cocultures also induced the levels of mRNA for connexin43 by 2.8-fold. We conclude that the interaction of HAEC, HASMC, and monocytes in coculture can result in marked increases in the levels of several biologically important molecules and that increased gap junction formation between the cells and interleukins 1 and 6 may be partially responsible for these changes. (J. Clin. Invest.

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Section: Discussionmentioning

confidence: 89%

Interaction of monocytes with cocultures of human aortic wall cells involves interleukins 1 and 6 with marked increases in connexin43 message.

Navab¹,

Liao²,

Hough³

et al. 1991

J. Clin. Invest.

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“…30 Fibronectin has been shown to be involved in inflammation. In animals, it is a positive acute-phase protein, with higher blood levels during inflammation, 31 but in humans, plasma FN is regarded as a negative acute-phase protein. 32 Fibronectin promotes chemotaxis and influences leukocyte function.…”

Section: The Involvement Of Fibronectin In Inflammatory Diseases and mentioning

confidence: 99%

Fibronectin and its soluble EDA-FN isoform as biomarkers for inflammation and sepsis

Lemańska-Perek¹,

Adamik²

2019

Adv Clin Exp Med

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“…More recently, IL-1p has been implicated in fever regulation through a direct action of the hypothalamus thermoregulation center and the stimulation of acute-phase protein synthesis by the liver (di Giovine and Duff, 1990). In addition, several recent studies have provided evidence that this cytokine may be an important regulator of connective tissue synthesis (Bitterman et al, 1986;Dedhar, 1989b;Duncan and Berman, 1989;Goldring and Krane, 1987;Hagiwara et al, 1989Hagiwara et al, , 1990. IL-1p stimulates proliferation of fibroblasts (Bitterman et al, 1986), affects collagen and fibronectin synthesis (Duncan and Berman, 1989;Goldring and Krane, 19871, modulates bone metabolism (Felix b;let al, 1989;Garrett and Mundy, 1989;Lorenzo et al, 1988), and induces chondrolysis (Saklatvala et al, 1985).…”

mentioning

confidence: 99%

Il‐1β and prostaglandins regulate integrin mRNA expression

Milam

Magnuson

Steffensen

et al. 1991

Journal Cellular Physiology

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The purpose of this study was to examine the effects of IL-1 beta on integrin expression in MG-63 human osteosarcoma cells. Human recombinant IL-1 beta (rIL-1 beta) produced significant increases in both alpha 2- and alpha 5-subunit mRNA levels, as well as a smaller increase in alpha v-subunit mRNA. In contrast, IL-1 beta decreased alpha 4-subunit mRNA levels by approximately 30% relative to untreated controls. These findings suggest that human IL-1 beta differentially regulates expression of integrins. When cultures were treated with both IL-1 beta and the cyclooxygenase inhibitor, indomethacin, the expression of alpha 2-, alpha 5-, and alpha v-subunit mRNA levels were dramatically increased relative to untreated controls; co-treatment with 0.5 mM prostaglandin E2 (PGE2) partially reversed this effect. Indomethacin alone did not affect integrin mRNA levels. Treatment with IL-1 beta or IL-1 beta + indomethacin also induced significant changes in MG-63 morphology (i.e., increased cell elongation) and increased the ability of cells to contract collagen gels. PGE2 reversed the above effects on cell morphology and gel contraction. These findings indicate that (a) IL-1 beta differentially regulates the expression of integrins and (b) that PGE2, which is induced by IL-1 beta, may provide a negative feedback loop which counteracts the stimulatory effect of IL-1 beta on integrin gene expression. It is suggested that products of inflammation may affect cell behavior by differentially regulating the expression of various integrins.

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Recombinant Interleukin-1 Triggers the Increase of Circulating Fibronectin Level in Rats

Cited by 5 publications

References 23 publications

Interaction of monocytes with cocultures of human aortic wall cells involves interleukins 1 and 6 with marked increases in connexin43 message.

Interaction of monocytes with cocultures of human aortic wall cells involves interleukins 1 and 6 with marked increases in connexin43 message.

Fibronectin and its soluble EDA-FN isoform as biomarkers for inflammation and sepsis

Il‐1β and prostaglandins regulate integrin mRNA expression

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