Recombinant medaka (Oryzias melastigmus) pro-hepcidin: Multifunctional characterization

Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro

“…Antiviral activity against both enveloped and non-enveloped viruses has been reported for several fish hepcidins [1,7,9,16,17,27]. Therefore, enveloped virus SCRV and nonenveloped virus MsReV were selected for studies of the antiviral activity of synthesized SA-hepcidins.…”

“…The antimicrobial activities of hepcidins from many fish species have been demonstrated, includinghepcidins from tilapia [6e12], turbot [13,14], black porgy [15], medaka [16], orange-spotted grouper [17], rare minnow [18], gilthead seabream [19], trout [20], large yellow croaker [21], zebrafish [22], channel catfish [23], Japanese flounder [24], half-smooth tongue sole [25], and sea bass [26]. Hepcidins used in antimicrobial analyses have generally been synthetic or recombinant peptides, which have consistently demonstrated antibacterial activity against Gram-positive and Gram-negative bacteria.…”

“…The most well-studied fish hepcidin is synthetic hepcidin TH1-5 from tilapia, which has antiviral activity against infectious pancreatic necrosis virus [27], Japanese encephalitis virus [9], and nervous necrosis virus [1,7]. Recombinant Pro-Omhep1 and synthetic Omhep1 from medaka have been shown to inhibit replication of the white spot syndrome virus [16]. Two EC-hepcidins from orange-spotted grouper inhibited replication of Singapore grouper iridovirus in fish cell lines [17].…”

Molecular cloning and mRNA expression of a hepcidin gene from the spinyhead croaker, Collichthys lucidus

“…Based on the knowledge that the expression of PC-hepc (158358729 L. crocea) is high in the kidney and relatively low in the liver, we assume that CL-hepc and PC-hepc are not the same isoform. Although different, all these HEPCs play important roles in innate immune responses (Shike et al, 2004;Bao et al, 2005;Wang et al, 2009;Cai et al, 2012;Qu et al, 2013). In addition, peptides obtained from purified, recombinant, and artificial synthesis all exhibit effective activity against several pathogens (Park et al, 2001;Bao et al, 2005;Wang et al, 2009).…”

Cloning and Functional Assessment of the Recombinant Human Hepcidin-25 in the Baculovirus Expression System

“…A previous study has shown that mouse hepcidin-1 produced in BES is able to lower the serum iron levels (Yazdani et al 2011). Several reports have described the cloning and expression of hepcidins in various species (Gerardi et al 2005;Zhang et al 2005;Gagliardo et al 2008;Greenshields et al 2008;Koliaraki, et al 2008;Lee et al 2008;Srinivasulu et al 2008;Cai et al 2012). The bacterial expression system is cost-effective, and generally the yields are high.…”