Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia

Nkando, Isabel; Perez-Casal, José; Mwirigi, Martin; Prysliak, Tracy; Townsend, H. G. G.; Berberov, Emil M.; Kuria, J. K. N.; Mugambi, J. M.; Soi, Reuben; Liljander, Anne; Jores, Joerg; Gerdts, Volker; Potter, Andrew; Naessens, Jan; Wesonga, Hezron

doi:10.1016/j.vetimm.2016.02.010

Cited by 22 publications

(23 citation statements)

References 42 publications

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“…Limited information is available about the type of immune response, key protective antigens, and optimal adjuvant formulations. Research on all possible antigens for their potential through reverse vaccinology ( Rappuoli, 2000 ) may be needed as this is more advanced method to identify recombinant proteins that provide protection against experimental challenge ( Perez-Casal et al., 2015 ; Nkando et al., 2016 ). Research demonstrated that MG vaccination in turkeys and chickens produced a high antibody titer including increased CD4 + and CD8 + expressing lymphocytes in trachea.…”

Section: Discussionmentioning

confidence: 99%

Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum

Ishfaq

Khan

et al. 2020

Poultry Science

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Mycoplasma gallisepticum ( MG ) is an important avian pathogen that causes significant economic losses in the poultry industry. Surprisingly, the limited protection and adverse reactions caused by the vaccines, including live vaccines, bacterin-based (killed) vaccines, and recombinant viral vaccines is still a major concern. Mycoplasma gallisepticum strains vary in infectivity and virulence and infection may sometimes unapparent and goes undetected. Although extensive research has been carried out on the biology of this pathogen, information is lacking about the type of immune response that confers protection and selection of appropriate protective antigens and adjuvants. Regardless of numerous efforts focused on the development of safe and effective vaccine for the control of MG, the use of modern DNA vaccine technology selected in silico approaches for the use of conserved recombinant proteins may be a better choice for the preparation of novel effective vaccines. More research is needed to characterize and elucidate MG products modulating MG-host interactions. These products could be used as a reference for the preparation and development of vaccines to control MG infections in poultry flocks.

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Section: Discussionmentioning

confidence: 99%

Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum

Ishfaq

Khan

et al. 2020

Poultry Science

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“…This involved location of trachea, after proper animal restraint, aseptic preparation of the inoculation site and introduction of the infectious agent into the trachea lumen. Earlier Mycoplasma infection studies in cattle employed the intra-tracheal intubation technique (Nkando et al, 2011(Nkando et al, , 2016. Advantages of the alternative method are: possible less stress exerted on the animal during the procedure, reduced execution time as well as zero use of Checking that the needle bevel is within the tracheal lumen by aspirating air using an empty syringe attached onto the introduced needle in (i) above and (iii) Administering of mycoplasma mycoides culture into the tracheal lumen.…”

Section: Discussionmentioning

confidence: 99%

“…pharmacological agents to calm the animal. In addition, the alternative method does not entail introduction of hot Mycoplasma agar down the trachea as shown in Nkando et al (2011) and Nkando et al, (2016). This is desirable when making a choice for an infection model, which is reproducible while mimicking the natural infection scenarios.…”

Section: Discussionmentioning

confidence: 99%

An alternative caprine infection model for Mycoplasma mycoides

Stolzmann¹,

Schieck²,

Liljander³

et al. 2019

Afr. J. Biotechnol.

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Ruminant infection models for Mycoplasma mycoides are invasive and laborious factors that may interfere with the experiment outcomes. This brief review presents an alternative method that may minimize on the mentioned challenges earlier mentioned. M. mycoides subsp. capri GM12 and M. mycoides subsp. capri YCP1.1 ∆68 strains were used; the former, causing a septicemia in goats. An intra-tracheal inoculation technique was employed after aseptic preparation as an alternative to the common intubation method. The former is cheaper, less laborious to handlers, with minimal stress to goats and more time-efficient. In conclusion, this may be used as an alternative to the intubation method. The described technique here will be useful in contagious bovine pleuropneumonia and contagious caprine pleuropneumonia research with modifications, diseases for cattle and small ruminants, respectively.

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“…Using this approach, we were able to identify recombinant proteins from Mycoplasma mycoides subsp. mycoides that conferred protection against an experimental challenge [67,68]. The host immune responses to M. bovis seem to be skewed towards a humoral (Th-2) response [10,11].…”

Section: Discussionmentioning

confidence: 99%