Recombinant osteoprotegerin effects during orthodontic movement in a rat model

Fernández-González, Felipe J.; Cañigral, Aránzazu; López-Caballo, José L; Brizuela-Velasco, Aritza; Cobo, Teresa; Carlos, Félix de; Galdames, Iván Suazo; Pérez-González, Yurena; Vega, J.A.

doi:10.1093/ejo/cjv056

Cited by 15 publications

(7 citation statements)

References 35 publications

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“…Group I (control) of 15 rabbits was injected with a 200 μl of phosphate-buffered saline (PBS) (MyBiosource, sunny Southern California, San Diego, USA), PBS widely used as a vehicle during injection of pharmacologic drug for orthodontic relapse control as described in previous studies. [ 16 17 ] Group II of 15 rabbits was injected with i-PRF 200 μl. Group III of 15 rabbits was injected with i-PRF 400 μl.…”

Section: Methodsmentioning

confidence: 99%

Effects of Injectable platelet rich fibrin (i-PRF) on reduction of relapse after orthodontic tooth movement

Al-Fakhry¹,

Al–Sayagh²

2022

Journal of Orthodontic Science

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OBJECTIVES: The objective of this study is to determine whether submucosal local injection of i-PRF may affect orthodontic relapse by increasing bone density, which further leads to reducing orthodontic relapse. MATERIALS AND METHODS: Forty-five adult male albino rabbits were randomly divided into three groups: group I (control) with 15 rabbits injected with 200 μl of phosphate-buffered saline (PBS), group II with 15 rabbits injected with 200 μl of i-PRF, and group III of 15 rabbits inject with 400 μl of i-PRF. The lower incisors of rabbits moved distally by a modified orthodontic appliance for 2 weeks; then, the appliance was maintained in position to retain the gaining space for 2 weeks. During the retention period, each group was injected with the specific drug every 7 days. After the retention period, teeth were allowed to relapse by removal of the orthodontic appliance. The results were evaluated by measuring the amount of orthodontic relapse and bone density. The statistical analysis performed by ANOVA and Duncan ( P < 0.05 was considered significant). RESULTS: I-PRF groups showed a significant reduction in the amount of relapse at 10, 13, 17, and 20 days compared to the control group, indicated by the highest percentage of relapse for the control group at the end of the study (20 days); it was (90.4%) in compared to lowest percentage of relapse for i-PRF groups—they were 61.2% and 59.9%, respectively. CONCLUSION: Results indicated that i-PRF has the potential to enhance the stability of teeth after orthodontic tooth movement and could have the ability to reduce relapse, probably by increasing the alveolar bone density.

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Section: Methodsmentioning

confidence: 99%

Effects of Injectable platelet rich fibrin (i-PRF) on reduction of relapse after orthodontic tooth movement

Al-Fakhry¹,

Al–Sayagh²

2022

Journal of Orthodontic Science

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“…The impression procedure was done using low viscosity Vinyl Polysiloxane wash material (Imprint II VPS Impression Material, 3 M ESPE, Irvine, USA) with mixing and intraoral tips (Fernández‐González et al, 2016; Miller et al, 2007). The light body impression material was injected around the maxillary molars with the aid of a cement spatula (Cement spatula, Sklar, West Chester, USA) that was used to reflect the cheeks and provide a clear and accurate impression.…”

Section: Methodsmentioning

confidence: 99%

The effects of calcitonin on post‐orthodontic relapse in rats

Alnajar

Groosh

2020

Clinical & Exp Dental Res

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Objectives To determine the effects of systematic calcitonin administration on post‐orthodontic relapse in rat model. Material and methods This experimental animal model involved 36 male Wister rats. The maxillary right first molars were moved mesially, using a modified orthodontic appliance delivered 50 gm, for 14 days, retained for 4 days and left to relapse for 10 days. The study group was divided into three subgroups in which a single injection of calcitonin (20 IU/Kg), three injections of calcitonin (20 IU/Kg), each every other day, and normal saline were administered subcutaneously after orthodontic tooth movement has finished. The relapse ratio, histomorphometric analysis including osteoblasts, osteoclasts numbers and bone area and immunohistochemical analysis including the expression of receptor activator of nuclear factor kappa Β (RANK), receptor activator of nuclear factor kappa Β ligand (RANKL) and osteoprotegerin (OPG) were measured and assessed. Results The relapse ratio was significantly reduced in the three‐dose calcitonin group (28%) compared to the single dose calcitonin group (34%) and the control group (46%). This was accompanied by a nonsignificant increase in osteoblasts number and bone area in three‐dose calcitonin group and a nonsignificant reduction in osteoclast number. However, the immune histochemical expression of RANK, RANKL and OPG did not show statistically significant difference at the end of relapse period. Conclusions Systemic administration of three doses of calcitonin may minimize the relapse ratio in experimentally moved rat molars.

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“…The controlled and sustained local release of RANKL from a NF-hydrogel carrier matrix was introduced and maximized its therapeutic benefit whilst minimizing systemic side effects, which maybe benefits further studies 186 . The effectiveness of recombinant OPG fusion protein was also assessed and it effectively inhibited osteoclastogenesis resulting in improved bone quantity and orthodontic anchorage 187 . The combination of bone homeostasis regulators and new drug delivery systems or methods is a promising direction for clinical application.…”

Section: Acceleration Of Otmmentioning

confidence: 99%

Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade

et al. 2021

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Nowadays, orthodontic treatment has become increasingly popular. However, the biological mechanisms of orthodontic tooth movement (OTM) have not been fully elucidated. We were aiming to summarize the evidences regarding the mechanisms of OTM. Firstly, we introduced the research models as a basis for further discussion of mechanisms. Secondly, we proposed a new hypothesis regarding the primary roles of periodontal ligament cells (PDLCs) and osteocytes involved in OTM mechanisms and summarized the biomechanical and biological responses of the periodontium in OTM through four steps, basically in OTM temporal sequences, as follows: (1) Extracellular mechanobiology of periodontium: biological, mechanical, and material changes of acellular components in periodontium under orthodontic forces were introduced. (2) Cell strain: the sensing, transduction, and regulation of mechanical stimuli in PDLCs and osteocytes. (3) Cell activation and differentiation: the activation and differentiation mechanisms of osteoblast and osteoclast, the force-induced sterile inflammation, and the communication networks consisting of sensors and effectors. (4) Tissue remodeling: the remodeling of bone and periodontal ligament (PDL) in the compression side and tension side responding to mechanical stimuli and root resorption. Lastly, we talked about the clinical implications of the updated OTM mechanisms, regarding optimal orthodontic force (OOF), acceleration of OTM, and prevention of root resorption.

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Recombinant osteoprotegerin effects during orthodontic movement in a rat model

Abstract: OPG-Fc effectively inhibits osteoclastogenesis resulting in improved bone quantity and orthodontic anchorage. Based on present results, OPG-Fc could have clinical utility in preventing undesired tooth movements.

Cited by 15 publications

References 35 publications

Effects of Injectable platelet rich fibrin (i-PRF) on reduction of relapse after orthodontic tooth movement

Effects of Injectable platelet rich fibrin (i-PRF) on reduction of relapse after orthodontic tooth movement

The effects of calcitonin on post‐orthodontic relapse in rats

Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade

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