Recombinant production of antimicrobial peptides in plants

Nazarian-Firouzabadi, Farhad; Torres, Marcelo Der Torossian; de la Fuente-Nunez, Cesar

doi:10.1016/j.biotechadv.2023.108296

Cited by 6 publications

(2 citation statements)

References 159 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This action is facilitated by the amphipathic nature of AMPs, which possess a balance of cationic and non-polar residues [ 16 ]. Unlike traditional antibiotics, which often target specific cellular processes and risk inducing resistance, the broad and nonspecific mode of action of AMPs makes it difficult for pathogens to develop resistance [ 17 ]. Additionally, some AMPs can penetrate cell membranes to target intracellular processes, further diversifying their mechanisms and reducing the likelihood of resistance [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight

Ran,

Shehzadi,

Liang

et al. 2024

IJMS

View full text Add to dashboard Cite

Soybean phytophthora blight is a severe menace to global agriculture, causing annual losses surpassing USD 1 billion. Present crop loss mitigation strategies primarily rely on chemical pesticides and disease-resistant breeding, frequently surpassed by the pathogens’ quick adaptive evolution. In this urgent scenario, our research delves into innovative antimicrobial peptides characterized by low drug resistance and environmental friendliness. Inhibiting chitin synthase gene activity in Phytophthora sojae impairs vital functions such as growth and sporulation, presenting an effective method to reduce its pathogenic impact. In our study, we screened 16 previously tested peptides to evaluate their antimicrobial effects against Phytophthora using structure-guided drug design, which involves molecular docking, saturation mutagenesis, molecular dynamics, and toxicity prediction. The in silico analysis identified AMP_04 with potential inhibitory activity against Phytophthora sojae’s chitin synthase. Through three rounds of saturation mutagenesis, we pin-pointed the most effective triple mutant, TP (D10K, G11I, S14L). Molecular dynamic simulations revealed TP’s stability in the chitin synthase-TP complex and its transmembrane mechanism, employing an all-atom force field. Our findings demonstrate the efficacy of TP in occupying the substrate-binding pocket and translocation catalytic channel. Effective inhibition of the chitin synthase enzyme can be achieved. Specifically, the triple mutant demonstrates enhanced antimicrobial potency and decreased toxicity relative to the wild-type AMP_04, utilizing a mechanism akin to the barrel-stave model during membrane translocation. Collectively, our study provides a new strategy that could be used as a potent antimicrobial agent in combatting soybean blight, contributing to sustainable agricultural practices.

show abstract

Section: Introductionmentioning

confidence: 99%

Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight

Ran,

Shehzadi,

Liang

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…AMPs make up a diverse group of small molecular peptides that are extensively distributed in the natural world. A total of 3100 natural AMPs have been identified within a diverse range of organisms, including bacteria, plants, insects, fish, birds, and other animals. , They play a crucial role in the innate immune system and act as the primary defense against pathogen infection . While they may not possess highly conserved sequences, the majority of these molecules are characterized by their short length, amphiphilic properties, and high cationic charge .…”

Section: Introductionmentioning

confidence: 99%

Screening of Short-Chain Antimicrobial Peptide LKARI with Broad-Spectrum Bactericidal Properties and Its Application in Promoting Wound Healing

Wu,

Wang,

Ren

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Discovery, Characterization, and Application of Broad-Spectrum Antimicrobial Peptide AtR905 from Aspergillus terreus as a Biocontrol Agent

Zhao,

Liu,

Lan

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

This study investigates a novel antimicrobial peptide AtR905 derived from the endophytic fungus Aspergillus terreus, which was successfully expressed in Bacillus subtilis, purified, and characterized, and highlighted as a promising potential biocontrol agent against various plant pathogens. The results indicated AtR905 exhibited broad-spectrum antimicrobial activities against key pathogens such as Ralstonia solanacearum and Clavibacter michiganensis with very low minimal inhibitory concentrations (MICs). Stability tests confirmed that AtR905 retains its antimicrobial properties under varying thermal, pH, and UV conditions. The Oxford Cup test indicated that AtR905 showed obvious fungicidal activity against six plant pathogenic fungi, especially Rhizoctonia solani and Botrytis cinerea. Additionally, in vivo experimental demonstrated AtR905 could effectively control the B. cinerea on tobacco leaves and R. solanacearum on tomato plants. Scanning electron microscopy revealed significant membrane disruption in bacterial cells treated with AtR905. These findings suggest that AtR905 is a promising candidate for sustainable plant disease management, potentially reducing the reliance on chemical pesticides and mitigating the issue of antibiotic resistance in agricultural settings. Further research is needed to evaluate the long-term field applicability and ecological impacts of AtR905.

show abstract

Recombinant production of antimicrobial peptides in plants

Cited by 6 publications

References 159 publications

Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight

Rationally Designed Novel Antimicrobial Peptides Targeting Chitin Synthase for Combating Soybean Phytophthora Blight

Screening of Short-Chain Antimicrobial Peptide LKARI with Broad-Spectrum Bactericidal Properties and Its Application in Promoting Wound Healing

Discovery, Characterization, and Application of Broad-Spectrum Antimicrobial Peptide AtR905 from Aspergillus terreus as a Biocontrol Agent

Contact Info

Product

Resources

About