Recombinant raccoon pox vaccine protects mice against lethal plague

Osorio, Jorge E.; Powell, Tim D.; Frank, Rexann S; Moss, Kelley J.; Haanes, Elizabeth J.; Smith, Susan R.; Rocke, Tonie E.; Stinchcomb, Dan T.

doi:10.1016/s0264-410x(02)00557-1

Cited by 39 publications

(52 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All prairie dogs were inoculated on the same day with dilutions of the same inocula. To prepare the inocula, a stock aliquot of the bacteria (the CO92 wild-type isolate of Y. pestis provided by the U.S. Army Medical Research Institute of Infectious Diseases), prepared and quantified as previously described (Osorio et al 2003), was diluted in phosphate-buffered saline to provide 50, 5000, and 50,000 cfu, which corresponds to 2.5, 250, and 2500 mouse LD 50 , respectively. A volume of 0.2 mL of this solution was administered to each prairie dog by subcutaneous injection in the right hip region.…”

Section: Y Pestis Challengementioning

confidence: 99%

Resistance to Plague Among Black-Tailed Prairie Dog Populations

Rocke

Williamson

Cobble

et al. 2012

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

In some rodent species frequently exposed to plague outbreaks caused by Yersinia pestis, resistance to the disease has evolved as a population trait. As a first step in determining if plague resistance has developed in black-tailed prairie dogs (Cynomys ludovicianus), animals captured from colonies in a plague-free region (South Dakota) and two plague-endemic regions (Colorado and Texas) were challenged with Y. pestis at one of three doses (2.5, 250, or 2500 mouse LD50s). South Dakota prairie dogs were far more susceptible to plague than Colorado and Texas prairie dogs ( p < 0.001), with a mortality rate of nearly 100% over all doses. Colorado and Texas prairie dogs were quite similar in their response, with overall survival rates of 50% and 60%, respectively. Prairie dogs from these states were heterogenous in their response, with some animals dying at the lowest dose (37% and 20%, respectively) and some surviving even at the highest dose (29% and 40%, respectively). Microsatellite analysis revealed that all three groups were distinct genetically, but further studies are needed to establish a genetic basis for the observed differences in plague resistance.

show abstract

Section: Y Pestis Challengementioning

confidence: 99%

Resistance to Plague Among Black-Tailed Prairie Dog Populations

Rocke

Williamson

Cobble

et al. 2012

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

show abstract

“…Prophylactic control of the disease through immunization could be more effective. Recently, Osorio et al (16) described a recombinant raccoon poxvirus (RCN) that expresses the F1 antigen of Y. pestis (herein designated RCN-F1) and protects mice from virulent plague challenge. In preliminary experiments with black-tailed prairie dogs (Cynomys ludovicianus), all animals vaccinated with RCN-F1 via intramuscular (i.m.)…”

mentioning

confidence: 99%

“…The raccoon poxvirus-vectored recombinant plague vaccine RCN-IRES-tPA-YpF1 (designated RCN-F1 in this paper) was produced as previously described (16) and stored at Ϫ70°C in 2-ml aliquots until bait production. Virus stocks were thawed and diluted to 5 ϫ 10 7 50% tissue culture infective doses (TCID 50 )/ml in Hanks' medium (Gibco BRL, Carlsbad, Calif.) supplemented with 5% glycerin (Sigma, St. Louis, Mo.)…”

mentioning

confidence: 99%

Protection of Black-Tailed Prairie Dogs (Cynomys ludovicianus) against Plague after Voluntary Consumption of Baits Containing Recombinant Raccoon Poxvirus Vaccine

et al. 2004

View full text Add to dashboard Cite

Prairie dogs (Cynomys spp.) are highly susceptible to Yersinia pestis and significant reservoirs of plague for humans in the western United States. A recombinant raccoon poxvirus, expressing the F1 antigen of Y. pestis, was incorporated into a palatable bait and offered to 18 black-tailed prairie dogs (Cynomys ludovicianus) for voluntary consumption; 18 negative control animals received placebo baits. Antibody titers against Y. pestis F1 antigen increased significantly (P < 0.01) in vaccinees, and their survival was significantly higher upon challenge with Y. pestis than that of negative controls (P < 0.01).

show abstract

“…3 In addition, previously published papers have suggested that conventional doses and techniques of radiotherapy are not sufficient to adequately control these particular and rare cases. [19][20][21] Functioning PGLs are very rare in the head and neck, although they are known to display neuroendocrine activity through secretion of catecholamines. 10,22 The sensitivities reported in patients with catecholaminesecreting PGLs are 74% for urine total metanephrines, 84% for norepinephrine, 18% for dopamine and 14% for epinephrine, but unfortunately no sensitivity has as yet been reported for methoxytyramine.…”

Section: Surgical Intervention and Initial Surveillancementioning

confidence: 99%

Clinical and biochemical responses after Gamma Knife surgery for a dopamine-secreting paraganglioma: case report

Tuleasca

Jaquet

Schweizer

et al. 2015

View full text Add to dashboard Cite

IntRoductIon: the efficacy of Gamma Knife surgery (GKs) in local tumor control of non-secreting paragangliomas (PGLs) has been fully described by previous studies. However, with regard to secreting PGL, only one previous case report exists advocating its efficacy at a biological level. cAse RePoRt: the aims of this study were: 1) to evaluate the safety/efficacy of GKs in a dopamine-secreting PGL; 2) to investigate whether the biological concentrations of free methoxytyramine could be used as a marker of treatment efficacy during the follow-up. we describe the case of a 62-year-old man diagnosed with left PGL. He initially underwent complete surgical excision. thirty months after, he developed recurrent biological and neuroradiological disease; the most sensitive biomarker for monitoring the disease, concentration of plasma free methoxytyramine, started to increase. GKs was performed at a maximal marginal dose of 16 Gy. during the following 30 months, concentration of free methoxytyramine gradually decreased from 0.14 nmol/l (2*uRL) before GKs to 0.09 nmol/l, 6 months after GKs and 0.07 nmol/l at the last follow-up after GKs (1.1*uRL), confirming the efficacy of the treatment. Additionally, at 30 months there was approximately 36.6% shrinkage from the initial target volume. concLusIon: the GKs treatment was safe and effective, this being confirmed clinically, neuroradiologically and biologically. the case illustrates the importance of laboratory tests taking into account methoxytyramine when analyzing biological samples to assess the biochemical activity of a PGL. In addition, the identification of methoxytyramine as a unique positive biomarker could designate it for the monitoring of tumor relapse after treatments, including Gamma Knife surgery.

show abstract

Recombinant raccoon pox vaccine protects mice against lethal plague

Cited by 39 publications

References 20 publications

Resistance to Plague Among Black-Tailed Prairie Dog Populations

Resistance to Plague Among Black-Tailed Prairie Dog Populations

Protection of Black-Tailed Prairie Dogs (Cynomys ludovicianus) against Plague after Voluntary Consumption of Baits Containing Recombinant Raccoon Poxvirus Vaccine

Clinical and biochemical responses after Gamma Knife surgery for a dopamine-secreting paraganglioma: case report

Contact Info

Product

Resources

About