Recombinant Sendai virus vectors for activated T lymphocytes

Okano, Shinji; Yonemitsu, Yoshikazu; Nagata, Shinji; Sata, Shihoko; Onimaru, Manabu; Nakagawa, Kei; Tomita, Yoshimi; Kishihara, Kenji; Hashimoto, Shuichi; Nakashima, Yutaka; Sugimachi, Keizō; Hasegawa, Mamoru; Sueishi, Katsuo

doi:10.1038/sj.gt.3301998

Cited by 27 publications

(20 citation statements)

References 44 publications

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“…min at 37˚C led to maximal expression of EGFP on day 2 (data not shown), as well as activated T cells (25). Taken together, SeVs can rapidly and efficiently infect human MoDC in a simple procedure, and they can provide maturation stimuli to immature MoDC.…”

Section: Bymentioning

confidence: 84%

“…In brief, 5 3 10 5 MoDC were incubated at an MOI of 300, if not specifically indicated, in 100 ml X-vivo 15 medium without serum in a 2-ml tube for 4 h before the addition of 1000 ml X-vivo 15 medium supplemented with 2% autologous serum for 48 h in a 24-well flat-bottom plate. To assess the infection/transduction efficiency and expression of costimulatory molecules, flow cytometry analysis was performed as previously described in detail (25).…”

Section: Infection To Modc and Flow Cytometry Analysismentioning

confidence: 99%

“…We have developed a viral gene transfer vector, using rSendai virus vectors (SeVs), and found a markedly superior gene transfer efficiency compared with findings with currently available vectors for use in various organs (23)(24)(25). We report in this study that SeV is an useful stimulant that can provide human DC with a continuous maturation signal via cytosolic RNA synthesis and reveal that both RIG-I-mediated signaling and CD4 T cell responses to helper Ags from SeV can overcome Treg suppression of effective priming of tumor-associated Ags (TAA)-specific CD8 T cells in the regulatory T cell-abundant setting.…”

mentioning

confidence: 99%

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Provision of Continuous Maturation Signaling to Dendritic Cells by RIG-I–Stimulating Cytosolic RNA Synthesis of Sendai Virus

Okano

Yonemitsu

Shirabe

et al. 2011

The Journal of Immunology

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Section: Bymentioning

confidence: 84%

Section: Infection To Modc and Flow Cytometry Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Provision of Continuous Maturation Signaling to Dendritic Cells by RIG-I–Stimulating Cytosolic RNA Synthesis of Sendai Virus

Okano

Yonemitsu

Shirabe

et al. 2011

The Journal of Immunology

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“…When no ESClike colony is obtained in the culture dish at an optimal time after SeV infection, the following should be considered. First, the confluency of the mononuclear cells before T cell activation may not be appropriate because optimal activation of T cells is critical for SeV infection 25,26 . Too high a density of mononuclear cells leads to cell death, interfering with the proper activation of T cells.…”

Section: Discussionmentioning

confidence: 99%

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Kishino¹,

Seki

Yuasa³

et al. 2015

JoVE

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Recently, iPSCs have attracted attention as a new source of cells for regenerative therapies. Although the initial method for generating iPSCs relied on dermal fibroblasts obtained by invasive biopsy and retroviral genomic insertion of transgenes, there have been many efforts to avoid these disadvantages. Human peripheral T cells are a unique cell source for generating iPSCs. iPSCs derived from T cells contain rearrangements of the T cell receptor (TCR) genes and are a source of antigen-specific T cells. Additionally, T cell receptor rearrangement in the genome has the potential to label individual cell lines and distinguish between transplanted and donor cells. For safe clinical application of iPSCs, it is important to minimize the risk of exposing newly generated iPSCs to harmful agents. Although fetal bovine serum and feeder cells have been essential for pluripotent stem cell culture, it is preferable to remove them from the culture system to reduce the risk of unpredictable pathogenicity. To address this, we have established a protocol for generating iPSCs from human peripheral T cells using Sendai virus to reduce the risk of exposing iPSCs to undefined pathogens. Although handling Sendai virus requires equipment with the appropriate biosafety level, Sendai virus infects activated T cells without genome insertion, yet with high efficiency. In this protocol, we demonstrate the generation of iPSCs from human peripheral T cells in feeder-free conditions using a combination of activated T cell culture and Sendai virus.

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“…Sendai virus is considered to be non-pathogenic to humans [17]. Efficient gene transduction into various primary cultured cells and tissues by recombinant virus vector has been reported [18][19][20][21][22]. Furthermore, it replicates in the cytoplasma and poses no risk of integration into the genomic DNA.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Sendai Viral Angiopoietin-1-Modified Mesenchymal Stem Cells for Ischemic Heart Disease

Huang¹,

Wang²,

Ham³

2011

Targets in Gene Therapy

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Recombinant Sendai virus vectors for activated T lymphocytes

Cited by 27 publications

References 44 publications

Provision of Continuous Maturation Signaling to Dendritic Cells by RIG-I–Stimulating Cytosolic RNA Synthesis of Sendai Virus

Provision of Continuous Maturation Signaling to Dendritic Cells by RIG-I–Stimulating Cytosolic RNA Synthesis of Sendai Virus

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Transplantation of Sendai Viral Angiopoietin-1-Modified Mesenchymal Stem Cells for Ischemic Heart Disease

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