Recombinant single-chain variable fragment and single domain antibody piezoimmunosensors for detection of HIV1 virion infectivity factor

Ferreira, Guilherme N. M.; Encarnação, João M.; Rosa, Luís F. M.; Rodrigues, Rogério M. M.; Breyner, Roberta; Barrento, Sara; Pedro, Luísa; Aires-da-Silva, Frederico; Gonçalves, João

doi:10.1016/j.bios.2007.04.022

Cited by 18 publications

(12 citation statements)

References 28 publications

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“…The successive contributions of solvents and adsorbed mass are thus obtained by a three parameter fitting (R m , L m , C 0 ) of the respective conductance functions (Encarnação et al, 2007b). This procedure is repeated for each stage of the experiment and the BVD parameters of the particular experimental stage, thus of the individual contributions, are calculated by subtracting the global parameters, obtained by fitting, from the respective parameter calculated for the previous stage of the experiment (Encarnação et al, 2007a(Encarnação et al, , 2007bFerreira et al, 2007). To facilitate data analysis it is usual to represent a parameter XL which is the sensor inductance in resistive units (V) obtained by multiplying the calculated inductance value by the angular frequency v ¼ 2pf.…”

Section: Impedance Analysismentioning

confidence: 99%

“…Piezoelectric transduction enables label-free detection of biorecognition events and has been used in microgravimetric devices, generally known as quartz-crystal microbalance (QCM), for different applications (Yang et al, 1998;Etchenique and Brudny, 2000;Liu et al, 2004;Su and Li, 2005;Wu et al, 2005;Modin et al, 2006;Encarnação et al, 2007a;Ferreira et al, 2007;Mitomo et al, 2007). These sensors are driven to mechanically resonate at a particular frequency ( f 0 ) (Kö blinger et al, 1995;Laricchia-Robbio and Revoltella, 2004), that is dependent on the deposition of mass according to the Sauerbrey equation (Sauerbrey, 1959) (Equation (1)).…”

Section: Introductionmentioning

confidence: 99%

“…The use of correlations to account for the influence to the sensor resonance frequency by the resistive component, owing to viscoelastic effects (Martin et al, 1991;Zhou et al, 2000) and by the parallel capacitive component (Encarnação et al, 2007b), due to the influence of charges, enables the elimination of these interferences and the exact quantification of the deposited mass by the Sauerbrey equation (Encarnação et al, 2007a, Ferreira et al, 2007.…”

Section: Introductionmentioning

confidence: 99%

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Piezoelectric biosensors assisted with electroacoustic impedance spectroscopy: a tool for accurate quantitative molecular recognition analysis

Encarnação

Baltazar

Stallinga

et al. 2008

J of Molecular Recognition

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In this work, electroacoustic impedance analysis based on a modified Butterworth-Van Dyke (BVD) model is used to complement resonance frequency measurements of piezoelectric crystal sensors for the identification and removal of interfering signals. This approach enables the accurate use of the Sauerbrey correlation to establish a direct relationship between mass deposited at the sensor surface and measured frequency variations. Kinetic models can thus be evaluated and binding constants estimated directly from the measured data. We further demonstrate the usefulness of this approach by applying it to the study of the formation of 11-hydroxy-1-undecanothiol self-assembled monolayers (SAM) as well as to the binding of streptavidin to immobilized biotin. Kinetic and equilibrium parameters were estimated from transient analysis, adsorption isotherms, Scatchard and Hill plots obtained from the frequency data for both the alkanethiol and streptavidin films. This strategy based on electroacoustic impedance assisted quartz-crystal microbalance (QCM) biosensors is expected to be a major contribution for the use of these piezoelectric devices as a reliable and cheap detection system that can easily be integrated into analytical techniques.

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Section: Impedance Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Piezoelectric biosensors assisted with electroacoustic impedance spectroscopy: a tool for accurate quantitative molecular recognition analysis

Encarnação

Baltazar

Stallinga

et al. 2008

J of Molecular Recognition

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“…Single-domain antibodies were also successfully used for preparing piezoimmunosensors for the detection of HIV-1 virions [81]. The possibility of displaying at high density and controlling the orientation of these binders on the sensor surfaces strongly increases the method sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological applications of recombinant single-domain antibody fragments

Marco

2011

Microb Cell Fact

149

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BackgroundSingle-domain antibody fragments possess structural features, such as a small dimension, an elevated stability, and the singularity of recognizing epitopes non-accessible for conventional antibodies that make them interesting for several research and biotechnological applications.ResultsThe discovery of the single-domain antibody's potentials has stimulated their use in an increasing variety of fields. The rapid accumulation of articles describing new applications and further developments of established approaches has made it, therefore, necessary to update the previous reviews with a new and more complete summary of the topic.ConclusionsBeside the necessary task of updating, this work analyses in detail some applicative aspects of the single-domain antibodies that have been overseen in the past, such as their efficacy in affinity chromatography, as co-crystallization chaperones, protein aggregation controllers, enzyme activity tuners, and the specificities of the unconventional single-domain fragments.

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“…In Table 1 we list a few examples of engineered antibody (e.g. scFv, Vh, Fab)-based immunoassays for detection of viruses, cytochrome P450s (catalytic cellular enzymes that oxidize organic substances), IgG by use of a QCM [23, 24], small molecules, toxins, prostate-specific antigen (PSA), and bacteria by SPR [22], and Her2 by use of a piezoelectric microcantilever [25] and other biosensors [26–28]. …”

Section: Scfv Immunosensors and Immunoassaysmentioning

confidence: 99%

Recombinant antibodies and their use in biosensors

2011

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Inexpensive, noninvasive immunoassays can be used to quickly detect disease in humans. Immunoassay sensitivity and specificity are decidedly dependent upon high-affinity, antigen-specific antibodies. Antibodies are produced biologically. As such, antibody quality and suitability for use in immunoassays cannot be readily determined or controlled by human intervention. However, the process through which high-quality antibodies can be obtained has been shortened and streamlined by use of genetic engineering and recombinant antibody techniques. Antibodies that traditionally take several months or more to produce when animals are used can now be developed in a few weeks as recombinant antibodies produced in bacteria, yeast, or other cell types. Typically most immunoassays use two or more antibodies or antibody fragments to detect antigens that are indicators of disease. However, a label-free biosensor, for example, a quartz-crystal microbalance (QCM) needs one antibody only. As such, the cost and time needed to design and develop an immunoassay can be substantially reduced if recombinant antibodies and biosensors are used rather than traditional antibody and assay (e.g. enzyme-linked immunosorbant assay, ELISA) methods. Unlike traditional antibodies, recombinant antibodies can be genetically engineered to self-assemble on biosensor surfaces, at high density, and correctly oriented to enhance antigen-binding activity and to increase assay sensitivity, specificity, and stability. Additionally, biosensor surface chemistry and physical and electronic properties can be modified to further increase immunoassay performance above and beyond that obtained by use of traditional methods. This review describes some of the techniques investigators have used to develop highly specific and sensitive, recombinant antibody-based biosensors for detection of antigens in simple or complex biological samples.

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Recombinant single-chain variable fragment and single domain antibody piezoimmunosensors for detection of HIV1 virion infectivity factor

Cited by 18 publications

References 28 publications

Piezoelectric biosensors assisted with electroacoustic impedance spectroscopy: a tool for accurate quantitative molecular recognition analysis

Piezoelectric biosensors assisted with electroacoustic impedance spectroscopy: a tool for accurate quantitative molecular recognition analysis

Biotechnological applications of recombinant single-domain antibody fragments

Recombinant antibodies and their use in biosensors

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