Recombinant spider silk as matrices for cell culture

Widhe, Mona; Bysell, Helena; Nystedt, Sara; Schenning, Ingrid; Malmsten, Martin; Johansson, Jan; Rising, Anna; Hedhammar, My

doi:10.1016/j.biomaterials.2010.08.061

Cited by 107 publications

(135 citation statements)

References 41 publications

Supporting

Mentioning

135

Contrasting

Order By: Relevance

“…Adhesion and proliferation of 3T3 fibroblasts seeded to spider silk woven steel frames were reported earlier by Kuhbier and colleagues [8]. A work of Widhe and colleagues has shown that adhesion of human fibroblast to matrixes of the recombinant miniature spider silk protein 4RepCis was successful using foam, fibers and mesh [13]. Best rate of binding was, however, reported for a combination of film, mesh, and fibers.…”

Section: Resultsmentioning

confidence: 64%

Spider Silk as Guiding Biomaterial for Human Model Neurons

Roloff¹,

Strauß

Vogt

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

Over the last years, a number of therapeutic strategies have emerged to promote axonal regeneration. An attractive strategy is the implantation of biodegradable and nonimmunogenic artificial scaffolds into injured peripheral nerves. In previous studies, transplantation of decellularized veins filled with spider silk for bridging critical size nerve defects resulted in axonal regeneration and remyelination by invading endogenous Schwann cells. Detailed interaction of elongating neurons and the spider silk as guidance material is unknown. To visualize direct cellular interactions between spider silk and neurons in vitro, we developed an in vitro crossed silk fiber array. Here, we describe in detail for the first time that human (NT2) model neurons attach to silk scaffolds. Extending neurites can bridge gaps between single silk fibers and elongate afterwards on the neighboring fiber. Culturing human neurons on the silk arrays led to an increasing migration and adhesion of neuronal cell bodies to the spider silk fibers. Within three to four weeks, clustered somata and extending neurites formed ganglion-like cell structures. Microscopic imaging of human neurons on the crossed fiber arrays in vitro will allow for a more efficient development of methods to maximize cell adhesion and neurite growth on spider silk prior to transplantation studies.

show abstract

Section: Resultsmentioning

confidence: 64%

Spider Silk as Guiding Biomaterial for Human Model Neurons

Roloff¹,

Strauß

Vogt

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Combined, these studies suggest that neuron-specific surface binding sites need to be presented with neuron-compatible mechanical support, in order to promote long-term neuronal growth and network formation. Furthermore, as suggested previously [62], other material properties such as morphology, roughness and wettability may play roles in regulating cell growth on silk films. While the present study only investigated flat films, future studies to compare various matrices for neuron growth and network formation may be useful.…”

Section: Discussionmentioning

confidence: 60%

Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Tang-Schomer

Huang

et al. 2015

Biomaterials

View full text Add to dashboard Cite

Recombinant spider silks produced in transgenic goat milk were studied as cell culture matrices for neuronal growth. Major ampullate spidroin 1 (MaSp1) supported neuronal growth, axon extension and network connectivity, with cell morphology comparable to the gold standard poly-lysine. In addition, neurons growing on MaSp1 films had increased neural cell adhesion molecule (NCAM) expression at both mRNA and protein levels. The results indicate that MaSp1 films present useful surface charge and substrate stiffness to support the growth of primary rat cortical neurons. Moreover, a putative neuron-specific surface binding sequence GRGGL within MaSp1 may contribute to the biological regulation of neuron growth. These findings indicate that MaSp1 could regulate neuron growth through its physical and biological features. This dual regulation mode of MaSp1 could provide an alternative strategy for generating functional silk materials for neural tissue engineering.

show abstract

“…Film-, foam-, fiber-or mesh scaffolds of the recombinant spidroin 4RepCT (Figure 1) offer 2D or 3D cell culture environments 35 which support attachment, high survival and maintained phenotype of human primary fibroblasts (Figure 2). Another recombinant spider silk protein (IF9) can be prepared into porous 3D scaffolds by salt leaching, which support ingrowth and proliferation of a mouse fibroblast cell line.…”

Section: In Vitro Studies Of Recombinant Spider Silk Scaffoldsmentioning

confidence: 99%

Current progress and limitations of spider silk for biomedical applications

et al. 2011

Self Cite

View full text Add to dashboard Cite

Spider silk is a fascinating material combining remarkable mechanical properties with low density and biodegradability. Because of these properties and historical descriptions of medical applications, spider silk has been proposed to be the ideal biomaterial. However, overcoming the obstacles to produce spider silk in sufficient quantities and in a manner that meets regulatory demands has proven to be a difficult task. Also, there are relatively few studies of spider silk in biomedical applications available, and the methods and materials used vary a lot. Herein we summarize cell culture- and in vivo implantation studies of natural and synthetic spider silk, and also review the current status and future challenges in the quest for a large scale production of spider silk for medical applications.

show abstract

Recombinant spider silk as matrices for cell culture

Cited by 107 publications

References 41 publications

Spider Silk as Guiding Biomaterial for Human Model Neurons

Spider Silk as Guiding Biomaterial for Human Model Neurons

Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Current progress and limitations of spider silk for biomedical applications

Contact Info

Product

Resources

About