Recombinant vaccinia virus LC16m0 or LC16m8 that expresses hepatitis B surface antigen while preserving the attenuation of the parental virus strain

Morita, Michio; Suzuki, Kota; Yasuda, Atsushi; Kojima, Asato; Sugimoto, Masanobu; Watanabe, Kazuhiko; Kobayashi, Hidesaburo; Kajima, K.; Hashizume, So

doi:10.1016/0264-410x(87)90012-0

Cited by 24 publications

(9 citation statements)

References 20 publications

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“…Our morphological observations of cell lines infected with RVVs that express the gag and gag-pol genes of HIV-I confirm this. The synthesis, transport and biological properties of the recombinant products of RVVs are very similar to those of native proteins (Morita et al, 1987;Watanabe et al, 1989;Yasuda et al, 1990). RVVs constructed here expressed the inserted HIV-1 gene segments and processed the gag-pol proteins as expected.…”

Section: Discussionmentioning

confidence: 99%

Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

Hoshikawa¹,

Kojima²,

Yasuda³

et al. 1991

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

Hoshikawa¹,

Kojima²,

Yasuda³

et al. 1991

Journal of General Virology

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“…Virus preparations were purified by sucrose density gradient ultracentrifugation and adjusted to 10 7 PFU/ml. Methods for virus detection, culturing the recombinant clones, and Western blotting of cell lysate were performed as previously described (15,23,24,26,41). The anti-SIV Gag monoclonal antibody (MAb) IB6 was kindly supplied by T. Sata, Department of Pathology, NIID, and by K. Ikuta, Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan (21).…”

Section: Methodsmentioning

confidence: 99%

Intravenous Inoculation of Replication-DeficientRecombinant Vaccinia Virus DIs Expressing Simian ImmunodeficiencyVirus Gag Controls Highly Pathogenic Simian-HumanImmunodeficiency Virus inMonkeys

Izumi

Ami²,

Matsuo

et al. 2003

J Virol

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To be effective, a vaccine against human immunodeficiency virus type 1 (HIV-1) must induce virus-specific T-cell responses and it must be safe for use in humans. To address these issues, we developed a recombinant vaccinia virus DIs vaccine (rDIsSIVGag), which is nonreplicative in mammalian cells and expresses the full-length gag gene of simian immunodeficiency virus (SIV). Intravenous inoculation of 10 6 PFU of rDIsSIVGag in cynomologus macaques induced significant levels of gamma interferon (IFN-␥) spot-forming cells (SFC) specific for SIV Gag. Antigen-specific lymphocyte proliferative responses were also induced and were temporally associated with the peak of IFN-␥ SFC activity in each macaque. In contrast, macaques immunized with a vector control (rDIsLacZ) showed no significant induction of antigen-specific immune responses. After challenge with a highly pathogenic simian-human immunodeficiency virus (SHIV), CD4؉ T lymphocytes were maintained in the peripheral blood and lymphoid tissues of the immunized macaques. The viral set point in plasma was also reduced in these animals, which may be related to the enhancement of virus-specific intracellular IFN-␥ ؉ CD8 ؉ cell numbers and increased antibody titers after SHIV challenge. These results demonstrate that recombinant DIs has potential for use as an HIV/AIDS vaccine.

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“…Deletion of the viral growth factor gene was reported to result in a virus o flower virulence [3]. Less virulent, temperature-sensitive mutants of the Elstree strain have also been used as carriers for foreign genes [21]. None of these viruses, however, was extensively tested in a large number of animals, much less in humans.…”

Section: Introductionmentioning

confidence: 99%

Partial deletion of the human host range gene in the attenuated vaccinia virus MVA

Altenburger

Süter

Altenburger

1989

Archives of Virology

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The genome of a strongly attenuated vaccinia virus strain, MVA, was investigated by Southern blot and sequence analyses. Three major deletions, relative to the WR strain, were localized in MVA DNA. The deletions occurred near both ends of the viral genome and one of them affected a 55 K as well as the 32 K human host range gene. Although more than two thirds of the host range gene were eliminated from the MVA, the virus could still multiply in certain human cells.

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Recombinant vaccinia virus LC16m0 or LC16m8 that expresses hepatitis B surface antigen while preserving the attenuation of the parental virus strain

Cited by 24 publications

References 20 publications

Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

Intravenous Inoculation of Replication-DeficientRecombinant Vaccinia Virus DIs Expressing Simian ImmunodeficiencyVirus Gag Controls Highly Pathogenic Simian-HumanImmunodeficiency Virus inMonkeys

Partial deletion of the human host range gene in the attenuated vaccinia virus MVA

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