Recombination between Vaccine and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus

Wang, Anping; Chen, Qi; Wang, Leyi; Madson, Darin M.; Harmon, Karen; Gauger, Phillip C.; Zhang, Jianqiang; Li, Ganwu

doi:10.3201/eid2512.191111

Cited by 49 publications

(49 citation statements)

References 9 publications

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“…Recombination between PRRSV strains has been described under laboratory and field conditions [ 22 , 23 ]. Recombination events are not exclusive to PRRSV field strains; these events have also been described involving vaccine strains [ 10 , 13 , 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…In a later study from the same group, animals infected with the recombinant strain did not show significant clinical signs, although the recombinant strain demonstrated increased excretion and transmission capabilities compared to parental vaccine strains [ 10 ]. Other recombinant strains that have been identified to date have derived from the recombination between PRRSV field strains [ 11 , 12 ] or between field strains and MLV vaccine strains [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the Impact of the Recombinant Porcine Reproductive and Respiratory Syndrome Virus Horsens Strain on the Reproductive Performance in Pregnant Sows

Genís¹,

Kvisgaard

Larsen

et al. 2020

Pathogens

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This study assessed the impact of a PRRSV (porcine reproductive and respiratory syndrome virus) recombinant strain (Horsens strain) on the reproductive performance of naïve pregnant sows in the last third of gestation. Fifteen sows were included: four negative reproductive controls (NTX), five infected with a PRRSV-1 field strain (Olot/91, T01), and six infected with the recombinant PRRSV-1 strain (Horsens strain, T02). Piglets were monitored until weaning. Reproductive performance was the primary variable. In sows, viremia and nasal shedding (T01 and T02 groups), and, in piglets, viral load in blood and in lungs, as well as macroscopic lung lesions (T01 and T02 groups), were the secondary variables. The reproductive performance results were numerically different between the two challenged groups. Moreover, viral loads in blood were 1.83 × 106 ± 9.05 × 106 copies/mL at farrowing, 1.05 × 107 ± 2.21 × 107 copies/mL at weaning from piglets born from T01 animals and 1.64 × 103 ± 7.62 × 103 copies/mL at farrowing, 1.95 × 103 ± 1.17 × 104 copies/mL at weaning from piglets born from T02 sows. Overall, 68.8% of T01 piglets and 38.1% of T02 piglets presented mild lung lesions. In conclusion, the results suggest that Horsens strain is less virulent than the field strain Olot/91 under these experimental conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of the Impact of the Recombinant Porcine Reproductive and Respiratory Syndrome Virus Horsens Strain on the Reproductive Performance in Pregnant Sows

Genís¹,

Kvisgaard

Larsen

et al. 2020

Pathogens

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“…RNA recombination has experimentally been proven by coinfections of cells or swine with two different strains of PRRSV (Liu et al, 2011;Yuan et al, 1999). During the recent epidemiological investigations, recombination has been detected between two wild-type PRRSV strains (Franzo et al, 2014;Kwon et al, 2019;Zhao et al, 2015), between wild-type PRRSV and MLV vaccine (Bian et al, 2017;Wang et al, 2019a;Zhou et al, 2018) or between MLV vaccine strains (Eclercy et al, 2019).…”

Section: Genomic Rna Recombinationmentioning

confidence: 99%

Molecular and Cellular Mechanisms for PRRSV Pathogenesis and Host Response to Infection

et al. 2020

Virus Research

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“…Consequently, we visualize the network with r = 5 to demonstrate the 3 previously reported signals of recombination, alongside an additional 2 undescribed recombination events to highlight how unique strains may be identified (Figure 4). RF-Net identified strain IA30788-R (GB043798, Figure 4B) that has recently been described as a putative recombinant between a wildtype strain (IA76950-WT) and a commercial vaccine (70). In this case, our inference excluded the suggested parental strain (P129, GenBank: AF494042), but RF-Net was able to detect an evolutionary signal associated with recombination, i.e., RF-Net can use incomplete sequence data and reveal significant biological events.…”

Section: Recombination and Prrsv Evolution In The Usa 2014 To 2021mentioning

confidence: 99%

The United States Swine Pathogen Database: integrating veterinary diagnostic laboratory sequence data to monitor emerging pathogens of swine

Anderson

Inderski

Diel

et al. 2021

Preprint

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Veterinary diagnostic laboratories annually derive thousands of nucleotide sequences from clinical samples of swine pathogens such as porcine reproductive and respiratory syndrome virus (PRRSV), Senecavirus A, and swine enteric coronaviruses. In addition, next generation sequencing has resulted in the rapid production of full-length genomes. Presently, sequence data are released to diagnostic clients for the purposes of informing control measures, but are not publicly available as data may be associated with sensitive information. However, public sequence data can be used to objectively design field-relevant vaccines; determine when and how pathogens are spreading across the landscape; identify virus transmission hotspots; and are a critical component in genomic surveillance for pandemic preparedness. We have developed a centralized sequence database that integrates a selected set of previously private clinical data, using PRRSV data as an exemplar, alongside publicly available genomic information. We implemented the Tripal toolkit, using the open source Drupal content management system and the Chado database schema. Tripal consists of a collection of Drupal modules that are used to manage, visualize, and disseminate biological data stored within Chado. Hosting is provided by Amazon Web Services (AWS) EC2 cloud instance with resource scaling. New sequences sourced from diagnostic labs contain at a minimum four data items: genomic information; date of collection; collection location (state or province level); and a unique identifier. Users can download annotated genomic sequences from the database using a customized search interface that incorporates data mined from published literature; search for similar sequences using BLAST-based tools; and explore annotated reference genomes. Additionally, because the bulk of data presently are PRRSV sequences, custom curation and annotation pipelines have determined PRRSV genotype (Type 1 or 2), the location of open reading frames and nonstructural proteins, generated amino acid sequences, the occurrence of putative frame shifts, and restriction fragment length polymorphism (RFLP) classification of GP5 genes. Genomic data from seven major swine pathogens have been curated and annotated. The resource provides researchers timely access to sequences discovered by veterinary diagnosticians, allowing for epidemiological and comparative virology studies. The result will be a better understanding on the emergence of novel swine viruses in the United States (US), and how these novel strains are disseminated in the US and abroad.

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Recombination between Vaccine and Field Strains of Porcine Reproductive and Respiratory Syndrome Virus

Cited by 49 publications

References 9 publications

Assessment of the Impact of the Recombinant Porcine Reproductive and Respiratory Syndrome Virus Horsens Strain on the Reproductive Performance in Pregnant Sows

Assessment of the Impact of the Recombinant Porcine Reproductive and Respiratory Syndrome Virus Horsens Strain on the Reproductive Performance in Pregnant Sows

Molecular and Cellular Mechanisms for PRRSV Pathogenesis and Host Response to Infection

The United States Swine Pathogen Database: integrating veterinary diagnostic laboratory sequence data to monitor emerging pathogens of swine

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