RecombinationLactococcus lactisexpressingHelicobacter pylorineutrophil-activating protein A attenuates food allergy symptoms in mice

Abstract

Background Food allergy has been a significant public health issue with growing severity, prevalence and limited treatments. The neutrophil-activating protein A subunit (NapA) of Helicobacter pylori, a Th1-activating immune modulator, has been shown to have therapeutic potential in allergic diseases. Objective We adopted Lactococcus lactis (L. lactis) as mucosal delivery vehicles of NapA and sought to determine the regulatory effect of recombinant bacterium (L. lactis NZ3900/pNZ8149-NapA) on food allergy in mice. Methods The NapA expression efficiency of recombinant bacterium were determined. The effects of recombinant bacterium on food allergy in Balb/c mice were also investigated. Results NapA were delivered and expressed efficiently via L. lactis. The intervention of the engineered bacterium ameliorated food allergy symptoms (acute diarrhea and intestinal inflammation) and decreased serum histamine levels. In addition, the secretion of OVA-specific IgG2a, IFN-γ was promoted and the level of IL-4, OVA-specific IgE was restrained. Conclusions The recombinant strain may attenuate food allergy in mice through immune regulatory effect. Thus, L. lactis NZ3900/pNZ8149-NapA may be a promising approach for preventing or treating food allergy.

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

“…OVA-A can cause overreaction of the intestinal immune system, resulting in a pathological and potentially fatal immune response, including abdominal pain, diarrhea, vomiting, urticaria, angioedema, and other typical symptoms. 34,35 In severe cases, it can cause anaphylactic shock, which is accompanied by a series of effects such as weight loss, hypothermia, and excessive lymphocyte proliferation. 36 In this study, LTA intervention increased weight gain and alleviated allergic symptoms in the gastrointestinal and respiratory tracts.…”

Recombinant Probiotic Preparations: Current State, Development and Application Prospects

“…The offspring were also exposed to intranasal administration of the strain. As a result, a decrease in allergic inflammation in the offspring was observed, and prevention with the use of gnotobionts was more effective [173].…”