Recomendaciones para el estudio microbiológico de tejidos preservados para implantes

Abstract

IntroducciónCada vez con mayor frecuencia existen patologías que requieren como parte de su terapia el uso de tejidos homólogos para implantes, los que han mejorado el pronóstico de estas enfermedades.Se ha descrito un aumento significativo del número de implantes de tejidos como válvulas Recomendaciones para el estudio microbiológico de tejidos preservados para implantes PATRICIA GARCÍA C., BÉATRICE HERVÉ E., CHRYSTAL JULIET L., EDUARDO TURNER G., CLAUDIO ARRETZ V., JULIO ARRIAGADA V., CLAUDIO RAFOLS DEL C., ALBERTO PÉREZ C., JORGE VILLEGAS C., GLORIA M. GONZÁLEZ G., PAULINA AGUIRRE H., JOSÉ MINGUELL U., MAURICIO OCQUETEAU P., ISABEL PIZARRO A., FRANCISCO ANDRIGUETTI C. y ALONSO RODRÍGUEZ B.Recommendations for the microbiological study of tissues preserved for implantsThe implant of tissues in Medicine has enhanced the prognosis and/or quality of life of several diseases, however its utilization is not free of risks for the receptor. Because of this, a carefully selection of live or cadaveric donors and the microbiological control of tissues before its utilization are very important procedures in securing the quality of the procedure. Contamination of tissues may be intrinsic (because of unnoticed infection in the donor) or extrinsic (after the tissues has been harvested). These recommendations focus into the microbiological control of extrinsic contaminations, through its handling during the tissues harvest or during its processing for preservation. Most frequently used implants in our country (cardiac valves, osseus tissue, skin, hematopoyetic precursor cells and cornea) are analyzed and methodology is described according to existing data in the literature and through a review of procedures employed in foreing centers. As no one of implants is completely sterile it is very important to analyze too the recommendations for handling contaminated cultures. As authors we expect that this document will be useful and in brief, we can exhibit national multicenter figures constructed with comparable methodologies.

Phylogeny of Leontopodium (Asteraceae) in China—with a reference to plastid genome and nuclear ribosomal DNA

“…Therefore, additional cp genome data must be analyzed to unveil the infrageneric taxonomic system, species delimitation, and interspecies systematic relationships of Leontopodium. Compared with genomic data, phylogenetic analysis based on DNA barcodes, such as trnL-F, rbcL, matK, trnK-matK, psbA-trnH, ITS, and ETS, can solve many taxonomic problems, among which ITS and ETS are important nuclear DNA fragments that are widely used in phylogenetic studies (Kuzmanovićet al, 2017;Malekmohammadi et al, 2017;Vicent et al, 2017;Zhou and Zhang, 2017;Garcıá et al, 2018;Asanuma et al, 2019;Hussain et al, 2019;Pirani et al, 2020;Tan et al, 2020;Zhou et al, 2020;Hashim et al, 2021). ITS and ETS have fast evolutionary rates and high interspecies variation, which are reportedly effective in discriminating closely related species with relatively recent divergences (Li et al, 2011).…”