Recommendations for Standardized Procedures for the Online and Offline Measurement of Exhaled Lower Respiratory Nitric Oxide and Nasal Nitric Oxide in Adults and Children—1999

doi:10.1164/ajrccm.160.6.ats8-99

Cited by 739 publications

(127 citation statements)

References 141 publications

(265 reference statements)

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“…Exhaled air was collected before spirometry according to the American Thoracic Society recommendations [23], by using a pressure-regulated flow-restricted apparatus (Sievers, Boulder, Colo., USA). In brief, subjects without a nose clamp were asked to inhale to total lung capacity from a NO-free air source and, without a breath hold, to exhale to residual volume into a Mylar bag, maintaining a mouth pressure of 10 mm Hg.…”

Section: Methodsmentioning

confidence: 99%

Exhaled Nitric Oxide in a Population Sample of Adults

Bommarito

Migliore²,

Bugiani³

et al. 2007

Respiration

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Background: The relationship between exhaled nitric oxide (FENO) and the diagnosis of asthma in the general adult population is not completely clear. Objectives: To investigate the association between FENO and asthma, after controlling for atopy and rhinitis, in a general population sample of adults (mean age 43 years). Methods: The cohort of subjects was a sample of subjects who gave their consent to participate in the European Community Respiratory Health Survey II study. Results: Atopy, rhinitis and current asthma were positively and current smoking was negatively associated with FENO. Multivariate analysis showed that asthma had a significant predictive effect on FENO (β = 0.53; 95% CI 0.21–0.84, p = 0.001), and the increase in FENO was significantly associated with the risk of current asthma (OR = 1.07, 95% CI 1.00–1.14) by the logistic regression model. Receiver-operater characteristic curve showed that FENO ≧18.5 ppb had the best combination of sensitivity (69.2%) and specificity (71%), with a positive predictive value of 24% and a negative predictive value of 95%, for the diagnosis of asthma. Conclusions: Measuring FENO seems to be suitable as an adjunct to questionnaire in the evaluation of asthma in the general population.

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Section: Methodsmentioning

confidence: 99%

Exhaled Nitric Oxide in a Population Sample of Adults

Bommarito

Migliore²,

Bugiani³

et al. 2007

Respiration

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“…The measurements were performed at an expiratory flow of 50 ml/s [12]. The duration of exhalation had to be at least 6 s to produce a stable NO level for 3 s. Three recorded F ENO measurements were obtained for each subject.…”

Section: Methodsmentioning

confidence: 99%

N-Acetyl-Beta-Hexosaminidase Activity in Asthma

Tomasiak

Tomasiak²,

Ziętkowski³

et al. 2008

Int Arch Allergy Immunol

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Background: N-acetyl-β-hexosaminidase (β-hex) is a lysosomal hydrolase, which is selectively secreted into the extracellular space by inflammatory cells. The aim of our study was to assess the activity of β-hex in the plasma of asthmatic patients, and to establish whether it correlates with asthma severity and airway inflammation. Methods: The study was conducted in a group of 46 asthmatic patients and 13 healthy volunteers. All study participants underwent analysis of exhaled nitric oxide and flow-volume spirometry. β-hex activity, peripheral blood eosinophils, total serum IgE and eosinophil cationic protein were analyzed in blood samples from all asthmatic patients and healthy volunteers. Results: β-hex activity was significantly higher in patients with severe or moderate asthma compared with healthy volunteers and was positively correlated with exhaled nitric oxide levels and serum eosinophil cationic protein in these groups of patients. There was no correlation between β-hex activity and forced expiratory volume in 1 s, blood eosinophil count or total serum IgE in these groups of asthmatics. Conclusions: Our results suggest that β-hex could take part in airway inflammation and remodeling in asthma. Our study is the first report in which the elevated activity of β-hex in subjects with asthma has been observed. However, more studies are needed to establish the precise role of this enzyme in asthma in humans.

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“…The measurements were performed at an expiratory flow of 50 ml/s [11]. The duration of exhalation had to be at least 6 s to produce a stable nitric oxide level for 3 s. All subjects had 3 F ENO measurements recorded.…”

Section: Methodsmentioning

confidence: 99%

The Role of Endothelium-Derived Mediators in Exercise-Induced Bronchoconstriction

Ziętkowski

Bodzenta-Łukaszyk²,

Tomasiak³

et al. 2007

Int Arch Allergy Immunol

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Background: Exercise-induced bronchoconstriction (EIB) is a highly prevalent condition whose pathogenesis and effect on airways remain unclear. The aim of the study was to evaluate the changes in endothelial-derived mediators following intensive exercise in asthmatic patients. Methods: The study was conducted in a group of 25 asthmatic patients (15 with EIB, 10 without EIB) and 8 healthy volunteers. Changes induced by intensive exercise in the plasma concentrations of endothelin-1 (ET-1), thrombomodulin and soluble E-selectin were determined. Moreover, the possible correlations of these measurements with the results of baseline lung function, bronchial hyperreactivity (BHR), exhaled nitric oxide (F_ENO), baseline eosinophil cationic protein, peripheral blood eosinophilia and total IgE were investigated. Results: In the group of asthmatics with EIB, statistically significantly higher baseline concentrations of ET-1 and soluble E-selectin compared with asthmatics with negative exercise test results were revealed. In this group of patients, ET-1, thrombomodulin and soluble E-selectin concentrations significantly increased after exercise. Baseline concentrations of ET-1 as well as an increase in soluble E-selectin concentrations 60 min after exercise showed a statistically significant correlation with baseline eosinophil cationic protein, F_ENO and BHR. The increase in plasma concentrations of ET-1 15 min after exercise and soluble E-selectin 60 min after exercise showed a statistically significant correlation with the increase in F_ENO and BHR to histamine 24 h after exercise. Conclusions: We suggest that during EIB changes in the function of pulmonary endothelium occur and these changes may influence inflammation of the airway and remodeling in asthmatic patients.

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Recommendations for Standardized Procedures for the Online and Offline Measurement of Exhaled Lower Respiratory Nitric Oxide and Nasal Nitric Oxide in Adults and Children—1999

Cited by 739 publications

References 141 publications

Exhaled Nitric Oxide in a Population Sample of Adults

Exhaled Nitric Oxide in a Population Sample of Adults

N-Acetyl-Beta-Hexosaminidase Activity in Asthma

The Role of Endothelium-Derived Mediators in Exercise-Induced Bronchoconstriction

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