Recommended Assay for Treatment of Chicks to Prevent Salmonella Colonization by ‘Competitive Exclusion’

Mead, G. C.; Barrow, Paul; Hinton, M.; Humbert, F.; Impey, C. S.; Lahellec, C.; Mulder, R.W.A.W.; Stavric, Stanka; Stern, Norman J.

doi:10.4315/0362-028x-52.7.500

Cited by 58 publications

(35 citation statements)

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“…O Fator de infectividade foi determinado através da leitura das placas, onde se calculou a relatividade do número de colônias, por amostra de 10 aves e o Fator de Proteção é o componente relativo ao número do fator de infectividade. O método utilizado foi o descrito por Mead et al (1989). Utilizou-se placas de petri, com meio de cultura MacConkey (MERK) para o isolamento das cepas; meio BHI ou caldo nutriente, no enriquecimento das culturas; água peptonada 0,10%, para o isolamento das cepas; PBS 0,1 M pH 7, tampão fosfato salino na diluição das amostras; meio XLD (Gram-negativo) específico para a identificação das salmonelas e álcool a 70%, na desinfecção do local de coleta.…”

Section: Tabelaunclassified

Uso de probiótico na prevenção de salmoneloses em frangos de corte

Rossi¹,

Padilha

Santos

et al. 2007

Ciênc. agrotec.

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RESUMODentre as principais zoonoses de origem bacteriana, transmitidas pelas aves, pode-se assinalar as salmoneloses causadas por salmonelas patogênicas, que causam toxinfecções alimentares e podem estar presentes em produtos e subprodutos de origem animal contaminados. Com o objetivo de buscar subsídios no sentido de aperfeiçoar os sistemas de prevenção e controle alternativos para esta zoonose, foi realizado um experimento com 780 frangos de corte, da linhagem Ross, abatidos aos 31 dias de idade, submetidos a três tratamentos, com 4 repetições de 65 aves cada. Os animais do tratamento um receberam o probiótico Mucosal starter culture e foram inoculados com Salmonella enteritidis (SPRING et al., 2000), na dose de 10 5 unidades formadoras de colônias, por mililitro. No tratamento dois-controle positivo-os animais somente foram inoculados com S. enteritidis, na mesma dose que no tratamento um. Os animais do tratamento três-controle negativo-não receberam inoculação de salmonelas e nem probiótico. Avaliou-se o peso vivo, o consumo de ração e foram estimadas a conversão alimentar, o fator de produção e a percentagem de contaminação por salmonela. Não foram detectadas diferenças significativas, entre os tratamentos, para os parâmetros zootécnicos e microbiológicos analisados. Os animais que receberam o probiótico não se mostraram mais eficientes quando inoculados com salmonelas patogênicas, mas apresentaram um melhor desempenho em relação ao padrão da linhagem Ross utilizada. Termos para indexação:Saúde pública, salmonela, zoonose, avicultura, aditivo. ABSTRACTSalmoneloses are zoonoses cause by pathogenic salmonellas very common in products of infected birds. A experiment was made with the main aim to consider the need to search subsidy and perfectly for control and prevention systems in the growth of disease in public health. 780 chickens from Ross line are used, they were 31-day-old, and three treatments have been applied to 4 repetitions of 65 birds each. The animals of treatment one received the probiotic Mucosal starter culture and were inoculated with Salmonella enteritidis in 10 5 units group colony forms/ militer. The treatment two was considered positive control and the animal were inoculated with S. enteritidis the same way that treatment one. The animals of treatment three did not receive probiotics and nor inoculated with salmonella. Some parameters were analyzed such as weight, food conversion , production factor and the percent of the contamination by salmonellas. There were not differences to the zootechnical and microbiological parameters for everyone treatments analysed. The animals that received probiotic did not show more efficient when inoculated with S. enteritidis, but they presented best performance that status from Ross line used.

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Section: Tabelaunclassified

Uso de probiótico na prevenção de salmoneloses em frangos de corte

Rossi¹,

Padilha

Santos

et al. 2007

Ciênc. agrotec.

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“…To standardize ST challenges, Mead et al (1989) termed this value the "infection factor" (IF) and introduced another criterion calculated by dividing the IF value of the untreated group by that of the CE product-treated group, which they designated the "protection factor" (PF). Mead et al, suggested that cultures with PF values ＜4 would be insufficient to mitigate Salmonella infection in commercial poultry flocks.…”

Section: Discussionmentioning

confidence: 99%

Nurmi-type Culture Prepared using Culture Media without <sub>L</sub>-Cysteine Enhances <i>Salmonella </i>Exclusion in Hatched Layer Chicks

et al. 2016

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To determine the influence of media composition on Salmonella exclusion of Nurmi-type cultures, two and four types of cultures in the first and second trial, respectively, were prepared from the cecal contents of conventional laying hens, and Salmonella exclusion was assessed in newly hatched chicks. In the first trial, modified Viande Levure (VL) broth or nutrient broth (NB) were used to prepare Nurmi-type cultures (N-VL and N-NB), which were administered to the newly hatched chicks. Twenty-four hours later, the chicks were challenged with Salmonella enterica Typhimurium EF85-9 (ST). ST recoveries (log 10 colony forming units/g of cecal contents) from the N-VL-, N-NB-, and control-treated groups 5 days after the challenge were 7.6±0.6, 0.9±1.9, and 7.7±0.4, respectively. The results suggested the influence of L-cysteine (Cys) present in the VL broth. Thus, we determined the effect of Cys in the second trial. We prepared two other cultures using VL broth without Cys (N-VL−Cys) and NB with Cys (NNB＋Cys). ST recoveries from the cecal contents of the N-VL-, N-VL−Cys-, and control-treated groups were 6.3± 0.9, 2.1±2.5, and 9.2±0.8, respectively. ST was not recovered from the N-NB-and N-NB＋Cys-treated groups. To identify bacteria with Salmonella exclusion activity, we isolated 41 bacterial strains from the ceca of N-NB-treated chicks without Salmonella challenge. Most isolates were identified as Enterococcus faecalis or E. mundtii based on 16S rRNA gene sequencing, and only four cultures excluded Salmonella. Therefore, VL broth containing Cys was not always required for preparing Nurmi-type cultures. The use of media prepared with Cys at the lowest possible concentration or without Cys would promote to enhance Salmonella exclusion from Nurmi-type cultures.

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“…This suggested that tests of one or two weeks duration (Mead et al, 1989) may not be sufficient to evaluate the effectiveness of competitive exclusion products.…”

Section: Discussionmentioning

confidence: 99%

Competitive exclusion ofSalmonella typhimuriumin broilers fed with vermicompost and complex carbohydrates

1998

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Vermicompost (VC) was produced by earthworms fed with fresh chicken faeces, and was earth-like in appearance and odour. In three experiments, VC was sprinkled on the first feed of newly-hatched broiler chicks. Treated and control groups were challenged on day 6 by the addition of seeder chicks that had been inoculated orally with Salmonella typhimurium. Chickens were killed at intervals during a 6-week period and were tested for colonization of the pathogen in the crop, caecum and internal organs.The VC-treated groups were significantly more resistant (p < 0.01) to colonization by S. typhimurium than the untreated controls. In one experiment, the VC treatment appeared to have conferred complete protection against colonization of both the crop and caecum.Colonization of the crop was increased transiently by addition to the diet of 2.5% fructooligosaccharide, xylo-oligosaccharide or transgalactosylated oligosaccharide. The influence of these carbohydrates on colonization of the caecum was variable.

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Recommended Assay for Treatment of Chicks to Prevent Salmonella Colonization by ‘Competitive Exclusion’

Cited by 58 publications

References 0 publications

Uso de probiótico na prevenção de salmoneloses em frangos de corte

Uso de probiótico na prevenção de salmoneloses em frangos de corte

Nurmi-type Culture Prepared using Culture Media without <sub>L</sub>-Cysteine Enhances <i>Salmonella </i>Exclusion in Hatched Layer Chicks

Competitive exclusion ofSalmonella typhimuriumin broilers fed with vermicompost and complex carbohydrates

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