Abstract.A new method for absolute measurement of the effective delayed neutron fraction, β eff , based on Microscopic Noise Experiments and the Two-Region Model was developed at the IPEN/MB-01 Research Reactor. In contrast with other techniques, the main advantage of this new methodology is to obtain the parameters in a purely experimental way, eliminating all parameters that are difficult to measure or calculate. Consequently, the uncertainties associated to these parameters are eliminated and the accuracy in β eff is improved. Experimentally, several Rossi-α and Feynman-α measurements were performed in the IPEN/MB-01 in a large subcritical interval. By adopting the present approach, the final results show that uncertainties of 0.67% and 0.97% on, respectively, β eff and Λ are achievable. The theory/experiment comparison of β eff shows that among the available nuclear data libraries JENDL3.3 has the best performance.