Reconciling pillars of transient gene expression: From DNA prep via media, reagent and cell line development to holistic process optimization

Transient gene expression (TGE) in animal cell cultures has been used for almost 30 years to produce milligrams and grams of recombinant proteins, virus-like particles and viral vectors, mainly for research purposes. The need to increase the amount of product has led to a scale-up of TGE protocols. Moreover, product quality and process reproducibility are also of major importance, especially when TGE is employed for the preparation of clinical lots. This work gives an overview of the different technologies that are available for TGE and how they can be combined, depending on each application. Then, a critical assessment of the challenges of large-scale transient transfection follows, focusing on suspension cell cultures transfected with polyethylenimine (PEI), which is the most widely used methodology for transfection. Finally, emerging opportunities for transient transfection arising from gene therapy, personalized medicine and vaccine development are reviewed.

show abstract

Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

Gutiérrez-Granados

Cervera

Kamen

et al. 2018

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Reconciling pillars of transient gene expression: From DNA prep via media, reagent and cell line development to holistic process optimization

Cited by 1 publication

References 4 publications

Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

Contact Info

Product

Resources

About