Reconsidering Oral Rabies Vaccine Bait Uptake Evaluation at Population Level: A Simple, Noninvasive, and Ethical Method by Fecal Survey Using a Physical Biomarker

Robardet, Emmanuelle; Rieder, Jonathan; Barrat, J; Cliquet, Florence

doi:10.7589/2018-02-045

Cited by 4 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, analysis of tetracycline deposition in the teeth of animals that have ingested ORV baits is intrusive, time consuming, and requires animal anesthesia [5]. Alternatively, fecal analyses do not require capture and handling of animals, and could be a useful noninvasive sample [47]. This study suggests that rabies antibody responses may be reliably predicted via analysis of the volatile metabolome in fecal samples.…”

Section: Discussionmentioning

confidence: 99%

Volatile metabolomic signatures of rabies immunization in two mesocarnivore species

Kimball

Volker²,

Griffin³

et al. 2019

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Rabies is a zoonotic disease caused by infection with rabies virus, which circulates naturally in several wild carnivore and bat reservoirs in the United States (US). The most important reservoir in the US from an animal and public health perspective is the raccoon (Procyon lotor). To prevent the westward expansion of a significant raccoon rabies epizootic along the eastern seaboard, an operational control program implementing oral rabies vaccination (ORV) has existed in the US since the 1990s. Recently, two vaccine efficacy studies conducted with raccoons and striped skunks (Mephitis mephitis) provided the opportunity to determine if volatile fecal metabolites might be used to non-invasively monitor ORV programs and/or predict virus protection for these species. The volatile metabolome is a rich source of information that may significantly contribute to our understanding of disease and infection. Fecal samples were collected at multiple time points from raccoons and striped skunks subjected to oral treatment with rabies vaccine (or sham). Intramuscular challenge with a lethal dose of rabies virus was used to determine protection status at six (raccoons) and 11 (skunks) months post-vaccination. In addition to fecal samples, blood was collected at various time points to permit quantitative assessment of rabies antibody responses arising from immunization. Feces were analyzed by headspace gas chromatography with mass spectrometric detection and the chromatographic responses were grouped according to cluster analysis. Cluster scores were subjected to multivariate analyses of variance (MANOVA) to determine if fecal volatiles may hold a signal of immunization status. Multiple regression was then used to build models of the measured immune responses based on the metabolomic data. MANOVA results identified one cluster associated with protective status of skunks and one cluster associated with protective status of raccoons. Regression models demonstrated considerably greater success in predicting rabies antibody responses in both species. This is the first study to link volatile compounds with measures of adaptive immunity and provides further evidence that the volatile metabolome holds great promise for contributing to our understanding of disease and infections. The volatile metabolome may be an important resource for monitoring rabies immunization in raccoons and striped skunks.

show abstract

Section: Discussionmentioning

confidence: 99%

Volatile metabolomic signatures of rabies immunization in two mesocarnivore species

Kimball

Volker²,

Griffin³

et al. 2019

PLoS Negl Trop Dis

View full text Add to dashboard Cite

show abstract

Rabies control in wild carnivores

Gilbert

Chipman

2020

Rabies

View full text Add to dashboard Cite

Recent Advances in Oral Vaccines for Animals

Zhong,

Chen,

Zhang

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

Compared to traditional injected vaccines, oral vaccines offer significant advantages for the immunization of livestock and wildlife due to their ease of use, high compliance, improved safety, and potential to stimulate mucosal immune responses and induce systemic immunity against pathogens. This review provides an overview of the delivery methods for oral vaccines, and the factors that influence their immunogenicity. We also highlight the global progress and achievements in the development and use of oral vaccines for animals, shedding light on potential future applications in this field.

show abstract

Reconsidering Oral Rabies Vaccine Bait Uptake Evaluation at Population Level: A Simple, Noninvasive, and Ethical Method by Fecal Survey Using a Physical Biomarker

Cited by 4 publications

References 9 publications

Volatile metabolomic signatures of rabies immunization in two mesocarnivore species

Volatile metabolomic signatures of rabies immunization in two mesocarnivore species

Rabies control in wild carnivores

Recent Advances in Oral Vaccines for Animals

Contact Info

Product

Resources

About