Reconstituted Basement Membrane Induces Glandular-like Morphogenesis but no Difference in ACTH Synthesis of Anterior Pituitary Cells.

Kim, Hyun Joon; Hwang, Il-Tae; Lee, Hankyung; Yoo, Yung Choon; Lee, Suk-Keun; Hwang, Douk-Ho; Lee, Byung-Lan

doi:10.1507/endocrj.47.771

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…Pituitaries were collected as described in Animals and treatments, and total RNA was extracted using Trizol reagent (Invitrogen) and then purified using an RNeasy kit (QIAGEN Inc., Valencia, CA). For the primary pituitary cell culture, anterior pituitary cells were isolated from 10-wk-old female C57BL/6 mice as previously described (18) with minor modification. Isolated cells were counted and plated on poly-l-lysinecoated culture dishes containing medium (20 mm HEPES and 0.3% BSA in DMEM) supplemented with 10% fetal bovine serum.…”

Section: Measurement Of Gonadotropin Mrna Expressionmentioning

confidence: 99%

Pituitary Gonadotroph Estrogen Receptor-α Is Necessary for Fertility in Females

et al. 2007

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Estrogens play a central role in regulating female reproduction throughout the reproductive axis, and the pituitary is one of the major targets of estrogen action. We hypothesized that estrogen receptor alpha (ERalpha) mediates estrogen action in the pituitary gonadotroph. To test this hypothesis, we generated a mouse line with a selective ERalpha deletion in the gonadotropin alpha-subunit (alphaGSU)-expressing pituitary cells (pituitary-specific ERalpha knockout; ERalpha(flox/flox) alphaGSU(cre)). Although the ERalpha(flox/flox) alphaGSU(cre) female mice maintain a basal level of serum LH and FSH and their ovulatory capacity is comparable to that in controls, they do not display regular estrous cycles and are infertile, indicating a potential disorder in regulating LH and/or FSH secretion. The ERalpha(flox/flox) alphaGSU(cre) female mice express equivalent levels of LHbeta and alphaGSU mRNA compared with wild-type mice as determined by microarray analysis. Taken together, these findings indicate that pituitary gonadotroph ERalpha carries out the effects of estrogens with regard to estrous cyclicity and ultimately fertility.

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Section: Measurement Of Gonadotropin Mrna Expressionmentioning

confidence: 99%

Pituitary Gonadotroph Estrogen Receptor-α Is Necessary for Fertility in Females

et al. 2007

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“…In the pituitary, immunoreactivity for Ln, as revealed with a polyclonal antiserum, detecting all Ln containing the ␥1-chain (17), was detected in both epithelial and vascular BM and was suggested to play a role in the morphogenesis of the gland (18). In the thyroid, Ln-1 and -10 appear to be constituents of epithelial BM (15,19), whereas in the adrenal cortex, Ln has been found in BM throughout the cortex and has been suggested to be required for ho-meostasis of the tissue (3,20,21).…”

Section: T He Adrenal Cortex Develops From Mesenchymal Cellsmentioning

confidence: 99%

Laminin Isoforms in Fetal and Adult Human Adrenal Cortex

Virtanen

Korhonen

Petäjäniemi

et al. 2003

The Journal of Clinical Endocrinology & Metabolism

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Laminin has been proposed to influence the function of human adrenal cortex. We have studied the distribution of laminin (Ln) chains using immunofluorescence in human fetal and adult adrenal cortex. In the fetal gland Ln ␣2-and ␣5-chains were weakly expressed in the definitive zone, whereas Ln ␣4-, ␤1-, and ␥1-chains occurred around vessels. In the adult gland, Ln ␣2-, ␣5-, and ␥1-chains were found in epithelial basement membranes (BM) in all cortical zones, Ln ␣4-chain in vessels, Ln ␤1-chain in outer zone, and Ln ␤2-chain in the two inner zones of the cortex, respectively. Among the integrins in adult gland, integrin ␣ 3 -subunit was confined to basal surfaces of

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“…On the other hand there was no difference in mRNA synthesis in normal cells between cells on plastic and on Matrigel [89].…”

Section: Corticotrophsmentioning

confidence: 73%

“…In cells that were grown as 3-D cell clusters in Matrigel ACTH production was probably similar to that from flattened forms on plastic suggesting that factors, other than morphologies and cell architecture, related to cell state may interact [89]. On the other hand there was no difference in mRNA synthesis in normal cells between cells on plastic and on Matrigel [89].…”

Section: Corticotrophsmentioning

confidence: 90%

Extracellular Matrix Proteins in the Anterior Pituitary Gland

Kanasaki¹

2011

TONEUROEJ

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Abstract; The importance of the extracellular matrix (ECM) has become recognised as an important component of the functioning of cells. However the roles of the ECM in the anterior pituitary gland have received little attention. The pituitary has the particular characteristics of possessing a number of endocrine cells as well as less well studied nonendocrine cells. Because each cell has specific and vital roles in the physiology being of an individual the ECM must also possess selective activities. The details of ECM behaviour have not yet been delineated in the pituitary and this review first considers general characteristics of ECM compounds and then examines the properties of peptides with regard to functioning of the cells of the pituitary. It is noted that there are variations between ECM associated with normal cells and with tumours, that there are both morphological and biochemical responses to alterations in the composition of substrate on which cells are grown, and that a development of further models of ECM will be required for continued advances in the understanding of the manner in which the pituitary behaves in normal, clinical and pathological circumstances. Overall, the observations suggest that modulation of the ECM provides opportunities for enhancing well-being of individuals.

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Reconstituted Basement Membrane Induces Glandular-like Morphogenesis but no Difference in ACTH Synthesis of Anterior Pituitary Cells.

Cited by 4 publications

References 11 publications

Pituitary Gonadotroph Estrogen Receptor-α Is Necessary for Fertility in Females

Pituitary Gonadotroph Estrogen Receptor-α Is Necessary for Fertility in Females

Laminin Isoforms in Fetal and Adult Human Adrenal Cortex

Extracellular Matrix Proteins in the Anterior Pituitary Gland

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