Reconstructing lifetime nitrogen baselines and trophic position of Cynoscion acoupa from δ15N values of amino acids in otoliths

Vane, Kim; Wallsgrove, Natalie J.; Ekau, Werner; Popp, Brian N.

doi:10.3354/meps12625

Cited by 14 publications

(11 citation statements)

References 71 publications

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“…Source amino acids (AAs) undergo little to no isotopic fractionation during metabolism due to minimal exchange with the metabolic amino acid pool 7 leaving amino acid δ 15 N values that largely reflect the isotopic composition of the N sources within the environment where the animal resided as the tissue was formed 8–10 . Trophic AAs undergo considerable isotopic fractionation as they are metabolized via transamination 11 resulting in an apparent stepwise increase in δ 15 N values that corresponds to the trophic level of the animal 11,12 .…”

Section: Introductionmentioning

confidence: 99%

“…Acid hydrolysis of proteinaceous tissue generally occurs through heating the sample material in a strong acid to 100°C–150°C for 1.5–10 h, thereby breaking peptide bonds and releasing the individual AAs from the proteins contained in the sample material 15 . Isolation of AAs from interfering sample material is performed if samples contain a complex matrix (e.g., bone, sediment, and carbonates) that can interfere with the analysis and is commonly performed using cation‐exchange chromatography 10,16 . The use of a cation‐exchange resin generally results in minimal isotopic fractionation of AAs and provides sufficient recovery of sample material to allow analysis 17 .…”

Section: Introductionmentioning

confidence: 99%

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Practical considerations for improved reliability and precision during determination of δ¹⁵N values in amino acids using a single combined oxidation–reduction reactor

Riekenberg

Meer

Schouten

2020

Rapid Comm Mass Spectrometry

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Rationale There has been increased interest in the measurement of δ15N values in amino acids (AAs) to gain simultaneous insight into both trophic relationships and the composition of biogeochemical sources used by producers at the base of the food web. A new combustion reactor design in gas chromatography/combustion isotope ratio mass spectrometry (GC/C‐irMS) equipment has brought to light variable outcomes in performance, highlighting the need for better information about best practices for new systems. Methods Precision for δ15N values in amino acids using the single combined oxidation–reduction reactor is improved across a sequence of analyses if the reactor is oxidized for a substantial period (2 h) and subsequently maintained throughout the sequence with 12–17 s seed oxidation before each run during GC/C‐irMS. A five‐point calibration curve using amino acids with a range of δ15N values from −2.4‰ to +61.5‰ was used in combination with a 13–15 amino acid mixture to consistently normalize measurements to internationally calibrated reference materials. Results Combining this oxidation method with normalization techniques using both internal and external standards provided a reliable throughput of ~25 samples per week. It allowed for a reproducible level of precision of <±0.5‰, n = 10 within a derivatized standard mixture across each sequence and an average sample precision of ±0.27‰ n = 3, which is lower than the analytical precision typically associated with δ15N values for amino acid analysis (<±1‰). Conclusions A few practical considerations regarding oxidation and conditioning of the combustion reactor allow for increased sequence capacity with the single combined oxidation–reduction reactor. These considerations combined with normalization techniques result in a higher throughput and reduced analytical error during the measurement of δ15N values in amino acids.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Practical considerations for improved reliability and precision during determination of δ¹⁵N values in amino acids using a single combined oxidation–reduction reactor

Riekenberg

Meer

Schouten

2020

Rapid Comm Mass Spectrometry

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“…This contrast with the situation described for Brazilian North coast, where Acoupa weakfish production is much larger and the attached socio-economic importance is much greater, and therefore there is more research on the species (e.g. Matos and Lucena, 2006;Rodrigues et al, 2008;Mourão et al, 2009, Almeida et al, 2011Barletta et al, 1998Barletta et al, , 2003Almeida, 2016;Ferreira et al, 2016;Vane et al, 2018). Thus, Acoupa weakfish fishery off São Paulo State may be characterized as "data-poor" (i.e.…”

Section: Discussionmentioning

confidence: 85%

The Fishery Exploitation of Acoupa Weakfish Off São Paulo Coast, Southeastern Brazil

et al. 2019

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The Acoupa weakfish Cynoscion acoupa (Lacepède, 1801) is a large inshore sciaenid of commercial and social importance found along the entire Brazilian coast. In spite of this, there is few information on the species, both in terms of biological aspects and fishery yields, particularly off southeastern Brazil. Within this context, this study aimed to an evaluation of Acoupa weakfish fishery production off São Paulo coast (23°22'-25°18'S) based on publicly available statistical data collected between 1998 and 2016. Acoupa weakfish fishery in this State takes place on both industrial and artisanal scales, and employs at least 22 different fishing gears. The pair-trawling fishery was the most important Acoupa weakfish producer during the period surveyed, with a sharp decline in the catches from 2007 onward due to the establishment of Marine Protected Areas off São Paulo coast, which banned trawling in depths shallower than 26 meters. Acoupa weakfish yields were relatively low in São Paulo when compared with other demersal fishes exploited, despite the high retail prices commanded by the species in regional markets and restaurants.

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“…The ground-breaking work by McMahon et al (2011aMcMahon et al ( , 2011bMcMahon et al ( , 2016 showed that this approach was possible in otoliths, and d 13 C values of amino acids effectively distinguished fish who fed on mangrove or seagrass food webs. More recently, Vane et al (2018) used d N of 'trophic' and 'source' amino acids to estimate ontogenetic trophic shifts of mobile fishes. This compound-specific approach is likely to become more frequently used as methods and instrumentation become more accessible.…”

Section: The Mediamentioning

confidence: 99%

The art of otolith chemistry: interpreting patterns by integrating perspectives

Walther¹

2019

Mar. Freshwater Res.

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The ability to obtain high-resolution chemical profiles across otoliths has expanded with technological advancements that prompted an explosion of data from diverse taxa in coastal, marine and freshwater systems worldwide. The questions pursued by most otolith chemists fall broadly into six categories: identifying origins, tracking migration, reconstructing environments, quantifying growth or physiology, validating ages and assessing diets. Advances in instrumentation have widened the periodic table of otolith elements, and two-dimensional mapping has further illuminated spatial heterogeneity across these complex structures. Although environmental drivers of observed elemental signatures in otoliths are often assumed to be paramount, multiple intrinsic and extrinsic factors can disrupt simple relationships between an element and a single environmental parameter. An otolith chemical profile is not a direct photograph of an environment, but rather an impressionistic image filtered through the multifaceted experiences of the fish itself. A ‘signal-to-noise’ approach that assesses the relative magnitudes of variation from intrinsic and extrinsic factors on chemical profiles may be a promising way to resolve the factor of interest against the ‘noise’ of others. A robust appreciation of environmental drivers, physiological regulation and calcification dynamics that affect the ability to effectively interpret otolith chemical patterns is necessary to drive the field forward.

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Reconstructing lifetime nitrogen baselines and trophic position of Cynoscion acoupa from δ15N values of amino acids in otoliths

Cited by 14 publications

References 71 publications

Practical considerations for improved reliability and precision during determination of δ¹⁵N values in amino acids using a single combined oxidation–reduction reactor

Practical considerations for improved reliability and precision during determination of δ¹⁵N values in amino acids using a single combined oxidation–reduction reactor

The Fishery Exploitation of Acoupa Weakfish Off São Paulo Coast, Southeastern Brazil

The art of otolith chemistry: interpreting patterns by integrating perspectives

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Reconstructing lifetime nitrogen baselines and trophic position of Cynoscion acoupa from δ15N values of amino acids in otoliths

Cited by 14 publications

References 71 publications

Practical considerations for improved reliability and precision during determination of δ15N values in amino acids using a single combined oxidation–reduction reactor

Practical considerations for improved reliability and precision during determination of δ15N values in amino acids using a single combined oxidation–reduction reactor

The Fishery Exploitation of Acoupa Weakfish Off São Paulo Coast, Southeastern Brazil

The art of otolith chemistry: interpreting patterns by integrating perspectives

Contact Info

Product

Resources

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Practical considerations for improved reliability and precision during determination of δ¹⁵N values in amino acids using a single combined oxidation–reduction reactor

Practical considerations for improved reliability and precision during determination of δ¹⁵N values in amino acids using a single combined oxidation–reduction reactor