Reconstruction of a Genome-Scale Metabolic Model of <i>Scenedesmus obliquus</i> and Its Application for Lipid Production under Three Trophic Modes

Ray, Ayusmita; Kundu, Pritam; Ghosh, Amit

doi:10.1021/acssynbio.3c00516

Cited by 8 publications

(4 citation statements)

References 80 publications

(145 reference statements)

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“…This iAR632 comprised 1467 reactions, 734 metabolites, and 632 genes. Predictions and experimental observations indicate that these genes exhibit a 3.8-fold increase in biomass and almost 4-fold higher lipid content under mixotrophic conditions compared to other trophic modes [51]. Therefore, S. Obliquus is a suitable candidate for PW treatment as it can grow in a mixotrophic environment and reduce TOC as proven in the ndings of this study.…”

Section: Total Organic Carbon (Toc) Removalsupporting

confidence: 56%

Produced Water Treatment by Semi-Continuous Sequential Bioreactor and Microalgae Photobioreactor

Khairuddin

2023

Preprint

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Produced water (PW) from oil and gas exploration has adverse effects on aquatic life and living organisms that need to be treated before discharge. However, proper treatment is essential to meet the permissible limits. In the study, the PW was first pre-treated with activated sludge. The pre-treated PW was then utilized as a feed for the Scenedesmus obliquus microalgae in a 13 L photobioreactor. Initially, 10% of the PW was introduced in 90% microalgae culture, and after exponential growth, an additional 25% of PW was added without any additional nutrients. This study has demonstrated the impact of semi-continuous treatment on the growth of microalgae as well as the removal of pollutants including total organic carbon (TOC) and heavy metals. Moreover, salinity, electrical conductivity (EC), pH, and total dissolved solids (TDS) of the PW-microalgae culture were assessed. The removal efficiency for TOC, TDS and EC was 64, 49.8, and 49.1% respectively. Furthermore, S. obliquus has effectively removed barium, iron, and manganese heavy metals 95, 76, and 52% respectively. S. obliquus has been found to be able to grow in a mixotrophic environment as it has reduced TOC in the PW.

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Section: Total Organic Carbon (Toc) Removalsupporting

confidence: 56%

Produced Water Treatment by Semi-Continuous Sequential Bioreactor and Microalgae Photobioreactor

Khairuddin

2023

Preprint

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“…S. obliquus (iAR632) has recently been studied for its biosynthetic pathways, revealing that this strain comprises 1467 reactions, 734 metabolites, and 632 genes. Predictions and experimental observations indicated a 3.8-fold increase in biomass and almost 4-fold higher lipid content under mixotrophic conditions than other trophic modes (Ray et al 2023 ).…”

Section: Resultsmentioning

confidence: 97%

Produced water treatment by semi-continuous sequential bioreactor and microalgae photobioreactor

Khairuddin,

Khan,

Sankaran

et al. 2024

Bioresour. Bioprocess.

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Produced water (PW) from oil and gas exploration adversely affects aquatic life and living organisms, necessitating treatment before discharge to meet effluent permissible limits. This study first used activated sludge to pretreat PW in a sequential batch reactor (SBR). The pretreated PW then entered a 13 L photobioreactor (PBR) containing Scenedesmus obliquus microalgae culture. Initially, 10% of the PW mixed with 90% microalgae culture in the PBR. After the exponential growth of the microalgae, an additional 25% of PW was added to the PBR without extra nutrients. This study reported the growth performance of microalgae in the PBR as well as the reduction in effluent’s total organic carbon (TOC), total dissolved solids (TDS), electrical conductivity (EC), and heavy metals content. The results demonstrated removal efficiencies of 64% for TOC, 49.8% for TDS, and 49.1% for EC. The results also showed reductions in barium, iron, and manganese in the effluent by 95, 76, and 52%, respectively.

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“…Various environmental conditions, such as the availability of nutrients, may trigger the accumulation of lipids in microalgae. This serves as a means of storing energy for future use after the stress has subsided [25].…”

Section: Discussionmentioning

confidence: 99%

The Future of Biofuel Sustainability: Lipid Production in Microbial Strains Explored by GC-MS Technique

Chaudhary,

Tripathi,

Kumar

et al. 2024

Preprint

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Background Microalgae helps purify wastewater and make energy. Assimilation of wastewater removes nitrogen and phosphorus from aquatic habitats. Biofuel, feedstock, fertilizers, and cosmetics benefit from their lipids, proteins, and carbohydrates. Cheap, efficient culture methods like linked microalgae aquaculture are growing in favor. Chlorella, Botryococcus, Chlorococcum, and Scenedesmus are lipid-producing microalgae with particular properties for biodiesel and nutraceuticals. Aims The research examined how temperature, light intensity, and pH affect lipid accumulation in four microalgal taxa to improve lipid synthesis. Methodology: To evaluate lipid production in microbial strain grown on BG-11 medium under different environmental conditions for 28 days. Variables included light intensity (3, 4, and 5 Klux), temperature (25 ± 2, 30 ± 2, 35 ± 20°C), and pH (6.0, 6.5, and 7.3). Each experiment had three dry-weight lipid sample processing replications. GC was used for fatty acid profiling. At certain temperatures, compounds were profiled on an HP-5MS column using helium as the carrier gas. Results Botryococcus, Chlorococcum, Chlorella, and Scenedesmus microalgal strains were examined for lipid production, fatty acid profile, and chemical component profile. Temperature, light, and pH impact lipid production. Temperature determined Botryococcus sp.'s maximum lipid production. Chlorella sp. produces lipids at all temperatures but benefits from light. High pH boosts lipid production in all strains. Statistical studies emphasize treatment-strain interactions because they affect results. Different strains have important and negligible fatty acids. Palmitic acid differentiates Botryococcus sp. microalgae contain hydrocarbon and fatty acid methyl esters. Conclusion The research examined the lipid production of four microalgal genera Chlorella, Botryococcus, Chlorococcum, and Scenedesmus at different temperatures and cultures. Better lipid synthesis parameters enhanced biofuel output and sustainability. The most lipid-rich microalgal strain was Botryococcus sp. at different temperatures, light intensities, and pH.

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Reconstruction of a Genome-Scale Metabolic Model of Scenedesmus obliquus and Its Application for Lipid Production under Three Trophic Modes

Cited by 8 publications

References 80 publications

Produced Water Treatment by Semi-Continuous Sequential Bioreactor and Microalgae Photobioreactor

Produced Water Treatment by Semi-Continuous Sequential Bioreactor and Microalgae Photobioreactor

Produced water treatment by semi-continuous sequential bioreactor and microalgae photobioreactor

The Future of Biofuel Sustainability: Lipid Production in Microbial Strains Explored by GC-MS Technique

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