Reconstruction of Action Potentials of Cardiac Cells from Extracellular Field Potentials

Application of k-Nearest Neighbors Method for Drug Concentration and Cardiotoxicity Classification Using Extracellular Field Potentials and Reconstructed Action Potentials of Cardiac Cells

“…Reconstuc�ng the waveform of the APs from FP recordings a�ected by DMSO compound using approach proposed in [11] allowed to determine parameters like RAPD 50 , RAPD 70 , RAPD 90 and their differences RAPD 90-50 and RAPD 90-70 , which allowed to further evaluate the effect of DMSO on the electrophysiology of cardiac cells. Fig.…”

“…Data prepara�on involved processing of FP recordings to ensure applicability of the dataset for ML training, feature extrac�on and class categoriza�on. Processing steps involved iden�fica�on and removal of artefacts, filtering of 50 Hz power supply noise using a complex filter based on wavelet denoising and PCA [11]. Feature extrac�on involved period separa�on and quan�fica�on of key FP parameters, such as interspike interval (ISI), FP dura�on (FPD) and maximum FP amplitude (FPA).…”

“…Feature extrac�on involved period separa�on and quan�fica�on of key FP parameters, such as interspike interval (ISI), FP dura�on (FPD) and maximum FP amplitude (FPA). Addi-�onally, FP recordings with mul�ple channels (DMSOtreated group) were used to mathema�cally reconstruct the AP using the approach from [11] allowing to consdider such parameters as RAP dura�ons at 50, 70 and 90% of the repolariza�on (RAPD50, RAPD70 and RAPD90) for model training (Fig. 1).…”

“…As an alterna�ve to invasive methods the AP can also be reconstructed mathe-ma�cally from the extracellular FPs recorded with unmodified MEA systems. The reconstructed APs (RAPs) not only allows to expand the number of parameters for cardiotoxicity assessment [11], but also can be used to expand the number of features for ML applica�ons.…”